CAJ | 학술논문

目的检测不育症患者精子凋亡率与精浆活性氧水平并分析其相关性.方法 92例男性不育症患者,分为精索静脉曲张(VC)组(n=32)、白细胞精液症组(n=30)和其他原因组(n=30);另取24例成功进行体外授精精液样本作为对照组.采用计算机辅助精液分析系统行精液常规检测(精液pH值、精子密度和精子活力),Annexin V/PI染色法检测精子凋亡率,TBA法检测精浆活性氧水平.比较不育症各组与对照组各精液参数的差异,分析各组精子凋亡率与精浆活性氧水平的相关性.结果不育症各组精子活力显著低于对照组(P<0.01),VC组和白细胞精液症组精子凋亡率和精浆活性氧水平显著高于对照组(P<0.05或P<0.01).白细胞精液症组精浆活性氧水平与精子凋亡率呈显著正相关(r=0.573,P<0.05).结论精子凋亡率和精浆活性氧水平升高可能与VC患者和白细胞精液症患者不育相关,白细胞精液症患者活性氧水平升高可能是引起精子凋亡率升高的重要因素之一.
목적 검측불육증환자정자조망솔여정장활성양수평병분석기상관성.방법 92례남성불육증환자,분위정색정맥곡장(VC)조(n=32)、백세포정액증조(n=30)화기타원인조(n=30);령취24례성공진행체외수정정액양본작위대조조.채용계산궤보조정액분석계통행정액상규검측(정액pH치、정자밀도화정자활력),Annexin V/PI염색법검측정자조망솔,TBA법검측정장활성양수평.비교불육증각조여대조조각정액삼수적차이,분석각조정자조망솔여정장활성양수평적상관성.결과 불육증각조정자활력현저저우대조조(P<0.01),VC조화백세포정액증조정자조망솔화정장활성양수평현저고우대조조(P<0.05혹P<0.01).백세포정액증조정장활성양수평여정자조망솔정현저정상관(r=0.573,P<0.05).결론 정자조망솔화정장활성양수평승고가능여VC환자화백세포정액증환자불육상관,백세포정액증환자활성양수평승고가능시인기정자조망솔승고적중요인소지일.
Objective To detect the sperm apoptosis rate and level of reactive oxygen species (ROS) in seminal plasma and explore their correlation among infertile males. Methods Ninety-two inferitile males were divided into varicocele (VC) group (n=32), leukocytospermia group(n=30) and the other cause group (n=30), and another 24 in vitro fertilization sperm samples were sereved as controls. The routine sperm parameters including seminal pH, sperm viability and sperm density were examined by computer assisted sperm analysis, the sperm apoptosis rate was asseseed using Annexin V/PI staining, and the ROS level in seminal plasma was detected by TBA method. The differences in seminal parameters between three infertile groups and control group were compared, and the correlation of sperm apoptosis rate with level of ROS in seminal plasma was explored in each group. Results The sperm viability of three infertile groups was significantly lower than that of control group (P<0.01). The sperm apoptosis rates and levels of ROS in seminal plasma in VC group and leukocytospermia group were significantly higher than those in control group (P < 0.05 or P < 0.01). The sperm apoptosis rate was positively correlated with the level of ROS in seminal plasma in leukocytospermia group(r=0. 573, P < 0.05). Conclusion The increased sperm apoptosis rate and level of seminal plasma ROS may be related to the infertility of patients with VC and leukocytospermia. The increased level of seminal plasma ROS may be one of the causes of increased sperm apoptosis rate in patients with leukocytospermia.