CAJ | 학술논문

背景与目的:有研究表明膜细胞骨架组织连接蛋白埃兹蛋白(Ezrin)能够促进肿瘤转移,可与上皮钙黏素相互作用,参与肿瘤细胞的转移过程.本研究探讨Ezrin和E-cadherin在食管鳞状细胞癌中的表达及与临床病理因素之间的关系,分析其在食管鳞癌中的诊断价值.方法:应用组织芯片技术免疫组织化学法(SP法)检测76例食管鳞状细胞癌患者组织标本及癌旁正常鳞状上皮中Ezrin和E-cadherin的表达情况,结合临床相关因素进行卡方检验及非参数检验等统计学分析.结果:76例食管癌旁正常鳞状上皮中Ezrin阳性35例(46.1%),E-cadherin阳性74例(97.4%);76例食管鳞癌组织中Ezrin阳性63例(82.9%),E-cadherin阳性34例(44.7%).Ezrin表达与食管鳞癌的浸润深度和淋巴结转移显著相关(P＜0.05);E-cadherin与肿瘤分化程度和淋巴结转移显著相关(P＜0.05),随肿瘤分化程度的降低,E-cadherin的表达缺失率逐渐增加.Ezrin表达升高和E-cadherin的表达缺失呈负相关.结论:Ezrin和E-cadherin均与食管鳞癌的发生、转移有关,且两者表达呈负相关.利用组织芯片技术联合检测Ezrin、E-cadherin的表达,有助于综合判断食管癌的恶性程度和转移潜能.
배경여목적:유연구표명막세포골가조직련접단백애자단백(Ezrin)능구촉진종류전이,가여상피개점소상호작용,삼여종류세포적전이과정.본연구탐토Ezrin화E-cadherin재식관린상세포암중적표체급여림상병리인소지간적관계,분석기재식관린암중적진단개치.방법:응용조직심편기술면역조직화학법(SP법)검측76례식관린상세포암환자조직표본급암방정상린상상피중Ezrin화E-cadherin적표체정황,결합림상상관인소진행잡방검험급비삼수검험등통계학분석.결과:76례식관암방정상린상상피중Ezrin양성35례(46.1%),E-cadherin양성74례(97.4%);76례식관린암조직중Ezrin양성63례(82.9%),E-cadherin양성34례(44.7%).Ezrin표체여식관린암적침윤심도화림파결전이현저상관(P＜0.05);E-cadherin여종류분화정도화림파결전이현저상관(P＜0.05),수종류분화정도적강저,E-cadherin적표체결실솔축점증가.Ezrin표체승고화E-cadherin적표체결실정부상관.결론:Ezrin화E-cadherin균여식관린암적발생、전이유관,차량자표체정부상관.이용조직심편기술연합검측Ezrin、E-cadherin적표체,유조우종합판단식관암적악성정도화전이잠능.
Background and Objective:It has been proven that Ezrin protein may interact with E-cadherin protein and take part in metastasis of tumor cells.This study was to investigate the expressions of Ezrin and E-cadherin in esophageal squamous cell carcinoma(ESCC)and their relationship with the clinicopathologic factors,and to analyze their diagnostic values for ESCC.Method:The expression of Ezrin and E-cadherin in 72 specimen of ESCC and the para-cancer normal squamous epithelium was detected using tissue array with SP immunohistochemistry.Their correlations with the clinicopathologic factors were analyzed statistically.Results:The positive rate of Ezrin was significantly higher in ESCC than in para-cancer normal squamous epithelium (90.7%vs.46.0%,P＜0.001);the positive rate of E-cadherin was significantly lower in ESCC than in para-cancer normal squamous epithelium (27.6%vs.97.4%.P＜0.001).Ezrin expression was related to the invasiveness and lymph node metastasis of ESCC(P＜0.05);E-cadherin expression was related to the differentiation and lymph node metastasis of ESCC(P＜0.05).The high expression of Ezrin was related to the low expression of E-cadherin(P＜0.05).Conclusion:The activation of Ezrin and the absence of E-cadherin contributes to the tumorigenesis and metastasis of ESCC.