CAJ | 학술논문

用海藻酸钠(SA)水凝胶将细胞色素c (cyt-c)固定在棱面热解石墨电极(EPPGE)表面,形成稳定的SA-cyt-c/EPPGE电极,采用蛋白膜伏安法(PFV)研究了cyt-c与电极之间的直接电化学和离子强度、pH、外加金属离子对其电化学行为的影响及其电催化性能. 结果表明:cyt-c中血红素辅基Fe(Ⅲ)/Fe(Ⅱ)电对表现出准可逆行为,pH 7.0、NaCl浓度为0.1 mol/L时电化学活性最高,外加金属离子则有抑制作用, SA-cyt-c/EPPGE可催化还原O_2,有可能发展成为一种溶解氧的生物传感器.
용해조산납(SA)수응효장세포색소c (cyt-c)고정재릉면열해석묵전겁(EPPGE)표면,형성은정적SA-cyt-c/EPPGE전겁,채용단백막복안법(PFV)연구료cyt-c여전겁지간적직접전화학화리자강도、pH、외가금속리자대기전화학행위적영향급기전최화성능. 결과표명:cyt-c중혈홍소보기Fe(Ⅲ)/Fe(Ⅱ)전대표현출준가역행위,pH 7.0、NaCl농도위0.1 mol/L시전화학활성최고,외가금속리자칙유억제작용, SA-cyt-c/EPPGE가최화환원O_2,유가능발전성위일충용해양적생물전감기.
Cytochrome c (cyt-c) entrapped in sodium alginate (SA) hydrogel was electrostatically bound to the surface of edge plane pyrolytic graphite electrode (EPPGE), and then a stable SA-cyt-c/EPPGE was prepared. Direct electrochemistry of cyt-c at the electrode was monitored by protein film voltammetry (PFV). The effects of ionic strength, pH and exterior substances on the direct electrochemistry, and electrocatalytic properties of cyt-c were also studied. The electrochemical behavior of heme Fe(Ⅲ)/Fe(Ⅱ) in cyt-c is quasi-reversible, and its electrochemical performance is the best when pH is 7.0 and the concentration of supporting electrolyte NaCl is 0.1 mol/L, however, it is inhibited by exterior metal ions. Catalytic reduction of oxygen could be achieved at the SA-cyt-c/EPPGE, which means that it might be developed into a kind of biological sensor for dissolved oxygen.