CAJ | 학술논문

目的观测梗阻性黄疸(OJ)大鼠血浆血栓素A_2(TXA_2)、前列环素I_2(PGI_2)及肾皮质微循环的变化,探讨OJ肾损伤的机制及丹参的治疗作用.方法将SD大鼠分为假手术组、胆总管结扎组(模型组)及丹参干预组(丹参组),每组分3、7、10天3个时相,每个时相8只.测定血浆TXA2、PGI2、血尿素氮(BUN)及肌酐(Cr)水平,计算T/P比值.应用WX-9型微循环显微镜观测各组相应时相肾皮质微血管管径的变化.结果模型组与假手术组比较各时相T/P值升高,肾皮质微血管收缩,7、10天血BUN、Cr水平升高(P＜0.05),且随胆道梗阻时间延长变化更加明显(P＜0.05).丹参组与模型组比较各时相T/P值降低,7、10天肾皮质微血管扩张,10天血BUN及Cr水平下降(P＜0.05).结论血T/P比值升高及肾脏微循环障碍是OJ肾损伤的重要原因,丹参注射液对梗阻性黄疸肾损伤有干预作用.
목적 관측경조성황달(OJ)대서혈장혈전소A_2(TXA_2)、전렬배소I_2(PGI_2)급신피질미순배적변화,탐토OJ신손상적궤제급단삼적치료작용.방법 장SD대서분위가수술조、담총관결찰조(모형조)급단삼간예조(단삼조),매조분3、7、10천3개시상,매개시상8지.측정혈장TXA2、PGI2、혈뇨소담(BUN)급기항(Cr)수평,계산T/P비치.응용WX-9형미순배현미경관측각조상응시상신피질미혈관관경적변화.결과 모형조여가수술조비교각시상T/P치승고,신피질미혈관수축,7、10천혈BUN、Cr수평승고(P＜0.05),차수담도경조시간연장변화경가명현(P＜0.05).단삼조여모형조비교각시상T/P치강저,7、10천신피질미혈관확장,10천혈BUN급Cr수평하강(P＜0.05).결론 혈T/P비치승고급신장미순배장애시OJ신손상적중요원인,단삼주사액대경조성황달신손상유간예작용.
Objective To observe the alteration of plasmal levels Of thromboxane A_2(TXA_2)and prostacyclin(PGI_2)as well as changes of microcirculation in renal cortex of obstructive jaundice model rats,and to study the effect of Radix Salviae miltiorrhizae(SM)on them.Methods SD rats were randomly divided into three groups:the sham operation group(A),the common bile duct ligation model group(B),and the SM treated model group(C),Their blood plasma TXA_2/PGI_2 ratio(T/P),blood levels of urea nitrogen(BUN)and creatinine(Cr)were determined respectively in batches(8 rats from each group)on the 3 rd,7 th and 10 th day,their capillary caliber(CC)in renal cortex was measured at the same time points using WX-9 type microcirculation microscope.Results Compared with Group A,T/P was higher and CC was smaller in Group B at all the time points.Levels of BUN and Cr increased on day 7 and day 10 after modeling(P＜0.05),and they were increasing markedly along with the longation of the obstructive time(P＜0.05).As compared with Group B,T/P was lower and CC was expanded in Group C,with levels of BUN and Cr lowered on day 10(P＜0.05).Conclusion T/P elevation and renal microcirculation obstacle are the important factors for inducing renal injury in obstructive jaundice,and SM shows a protective effect on kidney against the injury.