国际医学寄生虫病杂志
國際醫學寄生蟲病雜誌
국제의학기생충병잡지
INTERNATIONAL JOURNAL OF MEDICAL PARASITIC DISEASES
2011年
1期
28-32
,共5页
杨芳芳%张小娟%林睿%王鸽%黎学铭%欧阳颐
楊芳芳%張小娟%林睿%王鴿%黎學銘%歐暘頤
양방방%장소연%림예%왕합%려학명%구양이
钉螺%细胞色素氧化酶基因Ⅰ%系统进化树%亲缘关系
釘螺%細胞色素氧化酶基因Ⅰ%繫統進化樹%親緣關繫
정라%세포색소양화매기인Ⅰ%계통진화수%친연관계
Oncomelania hupensis%Cytochrome oxidase Ⅰ gene%Phylogenetic tree%Phylogenic relationship
目的 了解广西两地理株钉螺细胞色素氧化酶Ⅰ(cytochrome oxidase Ⅰ,CO Ⅰ)基因的差异性,探讨广西钉螺与周边邻近4省钉螺的亲缘关系.方法 采集广西靖西有肋、横县光壳钉螺,提取DNA,PCR扩增CO Ⅰ基因并测序.登录GenBank检索出云南大理、四川绵竹、湖南岳阳、湖北汉阳4地钉螺的CO Ⅰ基因序列.采用Clustal X软件对上述CO Ⅰ基因排序.采用拟钉螺CO Ⅰ基因序列作为外源性参考基因,Kimura双参数法计算遗传距离,Mega3.1软件中非加权组平均法(UPGMA)、邻接法(NJ)和最小进化法(ME)构建系统发生树.结果 广西靖西光壳钉螺与横县有肋钉螺CO Ⅰ基因的遗传距离为0.0505,同源性为95%.广西横县钉螺与湖南岳阳、湖北汉阳、四川绵竹、云南大理钉螺的遗传距离依次为0.0102、0.0171、0.1401、0.1421,CO Ⅰ基因的同源性依次为99%、98%、87%、86%.广西靖西钉螺与湖南岳阳、湖北汉阳、四川绵竹、云南大理钉螺的遗传距离依次为0.0451、0.0505、0.1382、0.1421,C0 Ⅰ基因的同源性依次为95%、95%、88%、87%.结论 广西有肋钉螺与光壳钉螺基因差异性较小.广西两种钉螺与湖南、湖北钉螺遗传距离较小,同源性较高.广西两种钉螺与四川、云南钉螺遗传距离较大,同源性较低.
目的 瞭解廣西兩地理株釘螺細胞色素氧化酶Ⅰ(cytochrome oxidase Ⅰ,CO Ⅰ)基因的差異性,探討廣西釘螺與週邊鄰近4省釘螺的親緣關繫.方法 採集廣西靖西有肋、橫縣光殼釘螺,提取DNA,PCR擴增CO Ⅰ基因併測序.登錄GenBank檢索齣雲南大理、四川綿竹、湖南嶽暘、湖北漢暘4地釘螺的CO Ⅰ基因序列.採用Clustal X軟件對上述CO Ⅰ基因排序.採用擬釘螺CO Ⅰ基因序列作為外源性參攷基因,Kimura雙參數法計算遺傳距離,Mega3.1軟件中非加權組平均法(UPGMA)、鄰接法(NJ)和最小進化法(ME)構建繫統髮生樹.結果 廣西靖西光殼釘螺與橫縣有肋釘螺CO Ⅰ基因的遺傳距離為0.0505,同源性為95%.廣西橫縣釘螺與湖南嶽暘、湖北漢暘、四川綿竹、雲南大理釘螺的遺傳距離依次為0.0102、0.0171、0.1401、0.1421,CO Ⅰ基因的同源性依次為99%、98%、87%、86%.廣西靖西釘螺與湖南嶽暘、湖北漢暘、四川綿竹、雲南大理釘螺的遺傳距離依次為0.0451、0.0505、0.1382、0.1421,C0 Ⅰ基因的同源性依次為95%、95%、88%、87%.結論 廣西有肋釘螺與光殼釘螺基因差異性較小.廣西兩種釘螺與湖南、湖北釘螺遺傳距離較小,同源性較高.廣西兩種釘螺與四川、雲南釘螺遺傳距離較大,同源性較低.
목적 료해엄서량지리주정라세포색소양화매Ⅰ(cytochrome oxidase Ⅰ,CO Ⅰ)기인적차이성,탐토엄서정라여주변린근4성정라적친연관계.방법 채집엄서정서유륵、횡현광각정라,제취DNA,PCR확증CO Ⅰ기인병측서.등록GenBank검색출운남대리、사천면죽、호남악양、호북한양4지정라적CO Ⅰ기인서렬.채용Clustal X연건대상술CO Ⅰ기인배서.채용의정라CO Ⅰ기인서렬작위외원성삼고기인,Kimura쌍삼수법계산유전거리,Mega3.1연건중비가권조평균법(UPGMA)、린접법(NJ)화최소진화법(ME)구건계통발생수.결과 엄서정서광각정라여횡현유륵정라CO Ⅰ기인적유전거리위0.0505,동원성위95%.엄서횡현정라여호남악양、호북한양、사천면죽、운남대리정라적유전거리의차위0.0102、0.0171、0.1401、0.1421,CO Ⅰ기인적동원성의차위99%、98%、87%、86%.엄서정서정라여호남악양、호북한양、사천면죽、운남대리정라적유전거리의차위0.0451、0.0505、0.1382、0.1421,C0 Ⅰ기인적동원성의차위95%、95%、88%、87%.결론 엄서유륵정라여광각정라기인차이성교소.엄서량충정라여호남、호북정라유전거리교소,동원성교고.엄서량충정라여사천、운남정라유전거리교대,동원성교저.
Objective To investigate the genetic difference of cytochrome oxidase Ⅰ (CO Ⅰ ) of two isolates of Oncomelania hupensis collected from Guangxi, and analyze the phylogenic relationship with isolates from 4 neighboring provinces. Methods Ribbed-shelled and smooth-shelled snails were collected from Jingxi County and Hengxian County of Guangxi Zhuang Autonomous Region respectively. Genomic DNA was extracted from the snails. CO Ⅰ gene fragments were amplified by PCR,then purified and sequenced. The data of CO Ⅰgene sequences of Yunnan,Sichuan,Hunan,and Hubei isolates were obtained from the GenBank. Sequence of each isolate was edited by using Clustal X software. The CO Ⅰ gene of Tricia was used as the reference of exogenous gene. The genetic distance was computed with Kimura method. The phylogenetic tree was constructed by the unweighted pair group method with arithmetic mean (UPGMA) as well as the methods of neighbor-joining (NJ) and minimum evolution (ME) with the MEGA (3.1) software. Results The genetic distance and homology of CO Ⅰ between Jingxi isolates (ribbed-shelled) and Hengxian isolates (smooth-shelled) was 0. 0505 and 95% ,respectively. The genetic distances of Hengxian isolate with other isolates from Yunnan, Sichuan, Hunan ,and Hubei Province were 0. 0102,0. 0171,0. 1401 and 0. 1421 ,with the homology of 99% ,98% ,87% and 86% respectively. Correspondingly,the genetic distances of Jingxi isolate with isolates from Yunnan,Sichuan,Hunan,and Hubei province were 0. 0451,0. 0505,0. 1382,and 0. 1421 ,while the homology was 95% ,95%,88% and 87% respectively. Conclusion No significant genetic difference was found between the ribbedshelled and smooth-shelled Oncomelania hupensis isolates from Guangxi Zhuang Autonomous Region. They were phylogenetically closer,and more homologous to the isolates from Hunan and Hubei Province. However,they showed longer genetic distance and less homologous to the isolates from Sichuan and Yunnan Province.
