CAJ | 학술논문

目的研究白介素17(interhukin-17,IL-17)抗体在吸烟所致慢性阻塞性肺疾病(chronic obstructive pulmonary disease,COPD)模型小鼠气道炎症中的作用.方法 C67/BL6雄性小鼠随机分为COPD组(8只)、COPD+IL-17抗体干预组(简称COPD+干预组,8只)和正常对照组(10只).对支气管肺泡灌洗液(BALF)进行细胞计数、染色和分类;用酶联免疫吸附试验检测小鼠肺组织匀浆IL-17水平,观察各组小鼠气道病理改变.结果 COPD组与COPD+干预组相比肺功能差异无统计学意义.COPD组、COPD+干预组小鼠与正常对照组相比BALF细胞总数显著增高[分别为(19.64±1.89)×104/ml,(15.47±2.99)×104/ml和(5.13±1.00)×104/ml,P＜0.01];COPD+干预组较COPD组细胞总数下降(P＜0.01);中性粒细胞比例[分别为(8.58+6.77)%,(22.98±8.46)%]及绝对值[(1.28±0.96)×104/ml,(4.53±1.73)×104/ml]显著下降(P值均＜0.01).肺组织HE染色病理评分COPD+干预组(73.25±18.58)较cOPD组(106.13±36.27)炎症有所减轻(P＜0.05).COPD+干预组小鼠肺组织匀浆中IL-17含量(0.084±0.041)pg/mg pro与COPD组(0.221±0.081)pg/mg pro相比显著降低(P＜0.01).结论吸烟所致COPD小鼠模型中IL-17参与了中性粒细胞引起的气道炎症,抑制IL-17的表达,可以减少气道内中性粒细胞的数量,减轻气道炎症.
목적 연구백개소17(interhukin-17,IL-17)항체재흡연소치만성조새성폐질병(chronic obstructive pulmonary disease,COPD)모형소서기도염증중적작용.방법 C67/BL6웅성소서수궤분위COPD조(8지)、COPD+IL-17항체간예조(간칭COPD+간예조,8지)화정상대조조(10지).대지기관폐포관세액(BALF)진행세포계수、염색화분류;용매련면역흡부시험검측소서폐조직균장IL-17수평,관찰각조소서기도병리개변.결과 COPD조여COPD+간예조상비폐공능차이무통계학의의.COPD조、COPD+간예조소서여정상대조조상비BALF세포총수현저증고[분별위(19.64±1.89)×104/ml,(15.47±2.99)×104/ml화(5.13±1.00)×104/ml,P＜0.01];COPD+간예조교COPD조세포총수하강(P＜0.01);중성립세포비례[분별위(8.58+6.77)%,(22.98±8.46)%]급절대치[(1.28±0.96)×104/ml,(4.53±1.73)×104/ml]현저하강(P치균＜0.01).폐조직HE염색병리평분COPD+간예조(73.25±18.58)교cOPD조(106.13±36.27)염증유소감경(P＜0.05).COPD+간예조소서폐조직균장중IL-17함량(0.084±0.041)pg/mg pro여COPD조(0.221±0.081)pg/mg pro상비현저강저(P＜0.01).결론 흡연소치COPD소서모형중IL-17삼여료중성립세포인기적기도염증,억제IL-17적표체,가이감소기도내중성립세포적수량,감경기도염증.
Objective To investigate the significance of interleukin-17(IL-17)antibodies in cigarette smoke-induced chronic obstructive pulmonary disease(COPD)in mice.Methods Male C57/BL6 mice were randomly divided into COPD group(n=8),COPD+anti-IL-17 Ab group(n=8),and normal control group (n=10).The Rumher of cells in bronchoalveolar lavage fluid(BALF)was calculated and the concentrations of IL-17 in lung tissues was measured by enzyme-linked immunosorbent assay.Histoiogic analysis of the lungs was done in each group.Results The intra-airway pressure(IP),peak expiratory flow(PEF),and Ip slope in anti-IL-17 Ab group were insignificant compared with those of COPD group.The total cell count in BALF was significantly higher in COPD group than in normal control group(P＜0.01).Compared with COPD group.the total cell count in COPD+anti-IL-17 Ab group was decreased[(19.64±1.89)×104/ml and(15.47±2.99)×104/ml(P＜0.01)],neutrophils in BALF were obviously decreased,(22.98±8.46)%,(8.58±6.77)%(P＜0.01).The airway inflammation was alleviated in COPD+anti-IL-17 Ab group by histologic analysis.The concentration of IL-17 in lung tissue was significantly lower in COPD+anti-IL-17 Ab group [(0.084±0.041)pg/mg Pro] than in the COPD group[(0.221±0.081)pg/mg pro](P＜0.01).Conclusions IL-17 might play a role in airway neutrophilic inflammation in cigarette smoke-induced COPD in mice.Anti-IL-17 could decrease the Rumbet of neutrophils in the BALF and attenuate the neutrophilic airway inflammation.