中华胃肠外科杂志
中華胃腸外科雜誌
중화위장외과잡지
CHINESE JOURNAL OF GASTROINTESTINAL SURGERY
2009年
6期
558-561
,共4页
周东雷%张鼎宇%胡旭光%陈丹磊%柯重伟%印慨%李际辉%郑成竹
週東雷%張鼎宇%鬍旭光%陳丹磊%柯重偉%印慨%李際輝%鄭成竹
주동뢰%장정우%호욱광%진단뢰%가중위%인개%리제휘%정성죽
糖尿病,2型%肠旁路术%胃切除术%葡萄糖激酶
糖尿病,2型%腸徬路術%胃切除術%葡萄糖激酶
당뇨병,2형%장방로술%위절제술%포도당격매
Diabetes mellitus,type 2%Intestinal bypass%Gastrectomy%Glucokinase
目的 探讨十二指肠空肠旁路手术(DJB)和袖状胃切除术(SG)对糖尿病大鼠肝脏葡萄激酶(GCK)表达的影响.方法 制作GK大鼠(非肥胖糖尿病大鼠)和正常SD大鼠DJB和SG两种术式的动物模型,动态观察术后空腹血糖和胰岛素的变化.术后8周取肝组织标本.分别采用实时定量RT-PCR和Western blot检测术后肝脏GCK mRNA和蛋白表达水平.结果 GK大鼠DJB组和SG组术后各时间点空腹血糖均显著低于术前水平(均P<0.01),假手术组1周内空腹血糖降低,2周后逐渐恢复到术前高水平;SD大鼠各种手术后血糖水平无明显改变(均P>0.05).GK大鼠DJB术后肝脏GCK mRNA和蛋白表达水平分别为1.45±0.29和494.25±30.25,SG术后分别为0.65±0.25和345.25±28.13:假手术组术后GCK mRNA和蛋白表达水平分别为1.05±0.19和409.13±26.86,与DJB组和SG组比较,差异有统计学意义(均P<0.01);对照组GCK mRNA和蛋白表达水平分别为1.04±0.17和404.75±30.90,与DJB组和SG组比较,差异具有统计学意义(均P<0.01).SD大鼠术后GCK表达也呈现了相似的变化.结论 DJB和SG均能明显改善糖尿病大鼠的术后血糖水平,但两种术式对肝脏GCK的表达却具有完全不同的影响.
目的 探討十二指腸空腸徬路手術(DJB)和袖狀胃切除術(SG)對糖尿病大鼠肝髒葡萄激酶(GCK)錶達的影響.方法 製作GK大鼠(非肥胖糖尿病大鼠)和正常SD大鼠DJB和SG兩種術式的動物模型,動態觀察術後空腹血糖和胰島素的變化.術後8週取肝組織標本.分彆採用實時定量RT-PCR和Western blot檢測術後肝髒GCK mRNA和蛋白錶達水平.結果 GK大鼠DJB組和SG組術後各時間點空腹血糖均顯著低于術前水平(均P<0.01),假手術組1週內空腹血糖降低,2週後逐漸恢複到術前高水平;SD大鼠各種手術後血糖水平無明顯改變(均P>0.05).GK大鼠DJB術後肝髒GCK mRNA和蛋白錶達水平分彆為1.45±0.29和494.25±30.25,SG術後分彆為0.65±0.25和345.25±28.13:假手術組術後GCK mRNA和蛋白錶達水平分彆為1.05±0.19和409.13±26.86,與DJB組和SG組比較,差異有統計學意義(均P<0.01);對照組GCK mRNA和蛋白錶達水平分彆為1.04±0.17和404.75±30.90,與DJB組和SG組比較,差異具有統計學意義(均P<0.01).SD大鼠術後GCK錶達也呈現瞭相似的變化.結論 DJB和SG均能明顯改善糖尿病大鼠的術後血糖水平,但兩種術式對肝髒GCK的錶達卻具有完全不同的影響.
목적 탐토십이지장공장방로수술(DJB)화수상위절제술(SG)대당뇨병대서간장포도격매(GCK)표체적영향.방법 제작GK대서(비비반당뇨병대서)화정상SD대서DJB화SG량충술식적동물모형,동태관찰술후공복혈당화이도소적변화.술후8주취간조직표본.분별채용실시정량RT-PCR화Western blot검측술후간장GCK mRNA화단백표체수평.결과 GK대서DJB조화SG조술후각시간점공복혈당균현저저우술전수평(균P<0.01),가수술조1주내공복혈당강저,2주후축점회복도술전고수평;SD대서각충수술후혈당수평무명현개변(균P>0.05).GK대서DJB술후간장GCK mRNA화단백표체수평분별위1.45±0.29화494.25±30.25,SG술후분별위0.65±0.25화345.25±28.13:가수술조술후GCK mRNA화단백표체수평분별위1.05±0.19화409.13±26.86,여DJB조화SG조비교,차이유통계학의의(균P<0.01);대조조GCK mRNA화단백표체수평분별위1.04±0.17화404.75±30.90,여DJB조화SG조비교,차이구유통계학의의(균P<0.01).SD대서술후GCK표체야정현료상사적변화.결론 DJB화SG균능명현개선당뇨병대서적술후혈당수평,단량충술식대간장GCK적표체각구유완전불동적영향.
Objective To investigate the effects of duodental-jejunal bypasss (DJB) and sleeve gastrectomy (SG) on the expression of liver glucokinase (GCK) in diabetic rats. Methods Animal models of Goto-Kakizaki rats and Sprague-Dawley rats were established by DJB and SG. Results of fasting glycemia and insulin were compared. Liver tissue was harvested 8 weeks postoperatively. Quantitative real-time PCR and Western blot were used to detect liver GCK mRNA and protein expression after operation. Results Fasting plasma glucose levels of DJB group and SG group in GK rats were markedly declined 3 day and 1, 2, 4, 6, 8 weeks postoperatively (all P<0.01), while Sham group only droped 3 day and 1 week postoperatively, and there were no significant differences 2 weeks postoperatively (P>0.05). Fasting plasma glucose levels of each group in SD rats did not ehange after operation. In GK rats, GCK mRNA level (1.45±0.29) and protein expression (494.25±30.25) after DJB were higher than Sham group (1.05±0.19 and 409.13±26.86) and control group (1.04±0.17 and 404.75± 30.90). GCK mRNA level and protein expression after SG were 0.65±0.25 and 345.25±28.13 respectively, which were significantly lower than those in control group (all P<0.01). All the groups in SD rats experienced similar GCK expression change. Conclusion Both DJB and SG can decrease the plasma glucose levels of GK rats, while there are different effects on the expression of liver GCK.
