CAJ | 학술논문

目的探讨鼠疫F1抗原胶体金检测试剂(简称金标试剂)的应用价值.方法制备金标试剂,实验室检测其灵敏度、特异性和稳定性.并在全国17个鼠疫监测点检测动物脏器标本1798份,以细菌培养和反向血凝法作为平行对照,比较金标试剂的符合率和阳性率.结果金标试剂灵敏度为0.0010g/L F1抗原,室温和4℃放置12个月检测效果稳定,检测假结核和小肠结肠炎菌悬液均呈阴性反应.现场检测标本,金标试剂与反向血凝法比较,符合率为97.11%(1746/1798),与细菌培养法比较,符合率为96.83%(1741/1798);而金标试剂阳性率为9.23%(166/1798),高于反向血凝法[6.79%(122/1798)]和细菌培养法[6.28%(113/1798)].结论金标试剂灵敏度高、特异性强,是可以用于鼠疫现场监测和病例判定的快速诊断方法.
목적 탐토서역F1항원효체금검측시제(간칭금표시제)적응용개치.방법 제비금표시제,실험실검측기령민도、특이성화은정성.병재전국17개서역감측점검측동물장기표본1798빈,이세균배양화반향혈응법작위평행대조,비교금표시제적부합솔화양성솔.결과 금표시제령민도위0.0010g/L F1항원,실온화4℃방치12개월검측효과은정,검측가결핵화소장결장염균현액균정음성반응.현장검측표본,금표시제여반향혈응법비교,부합솔위97.11%(1746/1798),여세균배양법비교,부합솔위96.83%(1741/1798);이금표시제양성솔위9.23%(166/1798),고우반향혈응법[6.79%(122/1798)]화세균배양법[6.28%(113/1798)].결론 금표시제령민도고、특이성강,시가이용우서역현장감측화병례판정적쾌속진단방법.
Objective To develop a rapid test for the detection of F1 antigen of Yersinia Pestis based on gold-immunochromatography.Methods F1 antibodies were coupled with colloidal gold to prepare collidal gold reagent,which was used to detect F1 antibodies based on double antigen sandwich.The collidal gold reagent was estimated for its sensitivity specificity and stablity in labs and 1798 samples were detected in 17 surveillance spots.Results The reagent was sensitive to 0.0010 g/L F1 antigens.The reagens kept stable when it had been placed at 4℃ or room-temperature for 12 months and did not react to Yersinia pseudotuberculosis and Yersinia enterolitica.In 17 surveillance labs the reagent was used to test 1798 viscera samples from animal.resulting an accordance rate of 97.11%(1746/1798)to bacterial culture and 96.83%(1741/1798)accordance to reverse indirect hemagglutination assay(RIHA),showing a higher detection rate[9.23%(166/1798)]compared with RIHA[6.79%(122/1798)]and bacterial culture[6.28%(113/1798)].Conclusions The collidal gold reagent,sensitive and specific in diagnosing Yersinia pestis infection of both human and animals,is a rapid method in surveillance spot.