CAJ | 학술논문

目的使用质子磁共振波谱(1H-MRS)研究大鼠创伤性轴索损伤(traumatic axonal injury,TAI)后组织代谢改变及其空间分布特征.方法使大鼠头颅发生线加速和角加速运动制作TAI模型.于伤前和伤后24 h采用多体素MRS方法检测大鼠脑内多个部位的组织代谢状态,分析伤后N-乙酰门冬氨酸(NAA)/总肌酸(Cr)、NAA/胆碱类化合物(Cho)和Cho/Cr值变化及NAA/Cr值变化的空间分布特征.采用免疫组化标记β淀粉样蛋白前体蛋白(β-APP)观察轴索损伤情况.结果与伤前相比,伤后24 h时NAA/Cr、NAA/Cho值显著下降(P<0.05).Cho/Cr值轻度升高(P>0.05);NAA/Cr值出现较大降低幅度的部位依次为脑干、海马、内囊、胼胝体和丘脑.病理学检查示这些部位出现轴索损伤.结论 TAI后脑组织出现严重代谢紊乱,以脑干、海马等部位变化最为显著.
목적 사용질자자공진파보(1H-MRS)연구대서창상성축색손상(traumatic axonal injury,TAI)후조직대사개변급기공간분포특정.방법 사대서두로발생선가속화각가속운동제작TAI모형.우상전화상후24 h채용다체소MRS방법검측대서뇌내다개부위적조직대사상태,분석상후N-을선문동안산(NAA)/총기산(Cr)、NAA/담감류화합물(Cho)화Cho/Cr치변화급NAA/Cr치변화적공간분포특정.채용면역조화표기β정분양단백전체단백(β-APP)관찰축색손상정황.결과 여상전상비,상후24 h시NAA/Cr、NAA/Cho치현저하강(P<0.05).Cho/Cr치경도승고(P>0.05);NAA/Cr치출현교대강저폭도적부위의차위뇌간、해마、내낭、변지체화구뇌.병이학검사시저사부위출현축색손상.결론 TAI후뇌조직출현엄중대사문란,이뇌간、해마등부위변화최위현저.
Objective To investigate the brain metabolic changes and evaluate their spatial distributions after traumatic axonal injury (TAI)in rats by using proton magnetic resonance spectroscopy(1H-MRS).Methods The TAI model was made by subjecting the head of the rats to the linear and angular accelerations.The multi-voxel MRS was employed to detect the tissue metabolic state at the levels of hippocampus-caudate and pons prior to injury and at 24 hours after injury.The alterations of NAA/Cr,NAA/Cho and Cho/Cr values as well as the spatial distribution of NAA/Cr reduction were accessed. Immunohistochemical staining for β-APP was used to observe the injured axons. Results A siguificantdecrease in NAA/Cr and NAA/Cho(P<0.05)and subtle increase in Cho/Cr(P>0.05)were observed in rats at 24 hours after TAI in comparison to the pre-injury levels.Notable decrease in NAA/Cr value was observed in the areas including the brain stem,hippocampus,internal capsule,corpus callosum and thalamus,where axonal injuries were confirmed by the histological examination. Conclusion Metabolic imbalances Occur in the brains of rats with TAI.with notable changes in the brain stem and the hippocampus.