CAJ | 학술논문

目的探讨过氧化物酶体增殖物激活受体γ(PPARγ)基因第6外显子C161→T单核苷酸多态性与老年男性骨质疏松的相关性.方法采用聚合酶链反应限制性片段长度多态性分析法检测老年男性骨质疏松组、老年男性非骨质疏松组(对照组)的基因频率分布;采用双能X线吸收测定法检测老年男性骨质疏松组及对照组腰椎和股骨上端(大转子、股骨颈)的骨密度;酶联免疫法(ELISA)检测血清骨钙素.结果 PPARγ基因第6外显子C161→T的多态性,有CC、CT和TT 3种基因型,老年男性骨质疏松组携带T等位基因的频率高于对照组,CT+TT分别为40.4%和25.7%(P＜0.05);与对照组比较,老年男性骨质疏松组骨钙素水平、骨密度较低;与CC基因型比较,携带T等位基因型的骨密度更低.结论 PPAR7基因多态性与老年男性骨质疏松有关,T等位基因是老年男性骨质疏松的易感因素.PPARγ可能是骨质疏松的一个候选基因.
목적 탐토과양화물매체증식물격활수체γ(PPARγ)기인제6외현자C161→T단핵감산다태성여노년남성골질소송적상관성.방법 채용취합매련반응한제성편단장도다태성분석법검측노년남성골질소송조、노년남성비골질소송조(대조조)적기인빈솔분포;채용쌍능X선흡수측정법검측노년남성골질소송조급대조조요추화고골상단(대전자、고골경)적골밀도;매련면역법(ELISA)검측혈청골개소.결과 PPARγ기인제6외현자C161→T적다태성,유CC、CT화TT 3충기인형,노년남성골질소송조휴대T등위기인적빈솔고우대조조,CT+TT분별위40.4%화25.7%(P＜0.05);여대조조비교,노년남성골질소송조골개소수평、골밀도교저;여CC기인형비교,휴대T등위기인형적골밀도경저.결론 PPAR7기인다태성여노년남성골질소송유관,T등위기인시노년남성골질소송적역감인소.PPARγ가능시골질소송적일개후선기인.
Objective To investigate the relationship between the single nucleotide polymorphisms of exon 6 C161→T of peroxisome proliferators activated receptor γ(PPART)gene and osteoporosis in elderly male. Methods Polymerase chain reaction restriction-fragment length polymorphism(PCR-RFLP)was used to analyze the gene frequency distribution in the groups of nonosteoporosis and osteoporosis in elderly male.Bone mineral density of lumbar and hip(neck of femur,greater trochanter,Ward's triangle)were measured by dual energy X-ray absorptiometry.Serum osteocalcin level was measured by enzyme-labeled immunosorbent assay(ELISA). Results The exon 6 of PPARγ had 3 genotypes(CC,CT and TT).The frequency of T allele in osteoporosis was higher than that in non-osteoporosis.Compared with the control group,serum bone glaprotein level and bone mineral density was lower in osteoporosis group.The bone mineral density in the genotype of CT and TT groups were lower than that in the genotype of CC group. Conclusions The study shows that the single nucleotide polymorphisms of 6th exons of PPARγ may relate to osteoporosis in elderly male.T allele of PPARγ may be a predisposing factor of osteoporosis in elderly male.PPARγmay be a candidate gene of osteoporosis in elderly males.