CAJ | 학술논문

目的探讨汉防己甲素(TET)对急性百草枯中毒所致肺损伤的拮抗作用.方法雄性Wistar大鼠随机分为对照组(7只)、未治疗组(32只)和治疗组(32只).治疗组与未治疗组大鼠用百草枯(15 mg/kg)一次性腹腔注射染毒.治疗组于百草枯染毒后6 h经口给予汉防己甲素30 mg/kg,1次/d;未治疗组给予等体积的生理盐水;对照组一次性腹腔注射等体积生理盐水.分别测定不同处理后3、7、14和21 d时大鼠血浆和肺匀浆中丙二醛(MDA)含量及超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活力,并观察肺组织结构改变.结果染毒3 d时,未治疗组血浆及匀浆中MDA含量分别为(3.65±0.44)nmol/ml、(9.54±0.92)nmol/mgpro,均高于对照组,差异有统计学意义(P<0.01);未治疗组3 d时血浆和3、7 d时匀浆中GSH-Px活力与对照组相比明显降低,差异有统计学意义(P<0.05);未治疗组3 d时血浆和14 d时匀浆中的SOD活力与对照组相比明显降低.差异有统计学意义(P<0.05);各时点治疗组血浆、匀浆中MDA含量与未治疗组比较,差异无统计学意义(P>0.05).3 d时治疗组血浆SOD活力与未治疗组相比明显增高,差异有统计学意义(P<0.01).治疗组血浆中GSH-Px活力虽均高于未治疗组,但差异均无统计学意义(P>0.05).治疗组肺纤维组织积分均低于未治疗组,差异有统计学意义(P<0.01).结论 TET对急性百草枯中毒大鼠血浆中SOD和GSH-Px活力降低有一定的拮抗作用,可减轻肺纤维化.
목적 탐토한방기갑소(TET)대급성백초고중독소치폐손상적길항작용.방법 웅성Wistar대서수궤분위대조조(7지)、미치료조(32지)화치료조(32지).치료조여미치료조대서용백초고(15 mg/kg)일차성복강주사염독.치료조우백초고염독후6 h경구급여한방기갑소30 mg/kg,1차/d;미치료조급여등체적적생리염수;대조조일차성복강주사등체적생리염수.분별측정불동처리후3、7、14화21 d시대서혈장화폐균장중병이철(MDA)함량급초양화물기화매(SOD)、곡광감태과양화물매(GSH-Px)활력,병관찰폐조직결구개변.결과 염독3 d시,미치료조혈장급균장중MDA함량분별위(3.65±0.44)nmol/ml、(9.54±0.92)nmol/mgpro,균고우대조조,차이유통계학의의(P<0.01);미치료조3 d시혈장화3、7 d시균장중GSH-Px활력여대조조상비명현강저,차이유통계학의의(P<0.05);미치료조3 d시혈장화14 d시균장중적SOD활력여대조조상비명현강저.차이유통계학의의(P<0.05);각시점치료조혈장、균장중MDA함량여미치료조비교,차이무통계학의의(P>0.05).3 d시치료조혈장SOD활력여미치료조상비명현증고,차이유통계학의의(P<0.01).치료조혈장중GSH-Px활력수균고우미치료조,단차이균무통계학의의(P>0.05).치료조폐섬유조직적분균저우미치료조,차이유통계학의의(P<0.01).결론 TET대급성백초고중독대서혈장중SOD화GSH-Px활력강저유일정적길항작용,가감경폐섬유화.
Objective To investigate the antagonistic efficacy of tetrandrine (TET) on lung injury induced by acute paraquat poisoning. Methods Male Wistar rats were randomly divided into three groups(control group, non-treatment group and treatment group). The tetrandrine of 30 mg/kg was given by gastrogavage six hours after 32 rats were intraperitoneally injected with paraquat 15mg/kg (treatment group). Then the same dose of tetrandrine was given once a day. Normal saline of the same volume was given by gastrogavage in another 32 rats intraperitoneally injected with paraquat 15 mg/kg (non-treatment group). Seven rats were intraperitoneally injected by normal saline as the control group. Levels of maleie dialdehyde (MDA), activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in plasma and the lung homogenate of three groups were determined at 3 d,7 d, 14 d and 21 d after exposure to paraquat. Histological changes of the lungs were observed. Results The levels of M DA at 3 d both in plasma [(3.65±0.44)nmol/ml] and the tung homogenate [(9.54±0.92 )nmol/mg pro] of the non-treatment group significantly increased compared with the control group (P<0.01), the activities of GSH-Px and SOD in plasma at 3 d were significantly less than the control group(P<0.05 or P<0.01), the activities of GSH-Px(3 d,7 d) and SOD(7 d, 14 d) in the lung homogenate were significantly less than the control group (P<0.05). There were no significant difference in the levels of MDA both in plasma and the lung homogenate between the treatment group and the non-treatment group (P>0.05). The SOD activities of treatment group on the third day was significantly increased compared with the non-treatment group(P<0.01 or P<0.05). Although the activities of GSH-Px in plasma and the lung homogenate of the treatment group on the third day were increased,there was no significantly difference compared with the non-treatment group (P>0.05). The integral score of pulmonary fibrosis in the treatment group were significantly lower than in the non-treatment group(P<0.01). Conclusion TET has antagonistic effect against acute toxieity of paraquat through significant reduction of pulmonary fibrosis.