中华胃肠外科杂志
中華胃腸外科雜誌
중화위장외과잡지
CHINESE JOURNAL OF GASTROINTESTINAL SURGERY
2012年
6期
629-632
,共4页
邵书先%廖秀军%张延祥%裘建明%张秀峰%杨关根
邵書先%廖秀軍%張延祥%裘建明%張秀峰%楊關根
소서선%료수군%장연상%구건명%장수봉%양관근
结直肠肿瘤%DNA甲基化%基因,Vimentin%基因,sFRP1%基因,HPP1
結直腸腫瘤%DNA甲基化%基因,Vimentin%基因,sFRP1%基因,HPP1
결직장종류%DNA갑기화%기인,Vimentin%기인,sFRP1%기인,HPP1
Colorectal neoplasms%DNA methylation%Gene,Vimentin%Gene,sFRP1%Gene,HPP1
目的 探讨联合检测Vimentin、sFRP1和HPP1基因甲基化对提高结直肠癌甲基化阳性率的意义.方法 收集90例结直肠癌(结直肠癌组)和60例腺瘤性息肉(腺瘤组)患者及20例结直肠正常组织(正常对照组)标本,提取组织标本的DNA,采用甲基化特异性PCR(MSP)检测Vimentin、sFRP1和HPP1基因甲基化状态,分析其与结直肠癌的关系及甲基化阳性率.结果 结直肠癌组Vimentin、sFRP1和HPP1基因甲基化率分别为66.7％(60/90)、68.9％ (62/90)和72.2％(65/90);腺瘤组则分别为53.3％(32/60)、55.0％( 33/60)和50.0％ (30/60);正常对照组分别为0、0和5.0％(1/20).结直肠癌组3个基因甲基化阳性率均高于腺瘤组和正常对照组(P＜0.05).3个基因甲基化联合检测诊断结直肠癌和腺瘤的阳性率分别为93.3％(84/90)和76.7％(46/60),高于单个基因检测的甲基化阳性率(P＜0.05).3个基因甲基化状态与本组结直肠癌患者的性别、年龄、肿瘤部位、淋巴结转移、远处转移及TNM分期无关(P＞0.05).结论 Vimentin、sFRP1和HPP1基因启动子甲基化水平在结直肠癌组织中升高,其联合检测明显提高了结直肠癌甲基化检测阳性率,有可能成为结直肠癌早期诊断的甲基化检测方案.
目的 探討聯閤檢測Vimentin、sFRP1和HPP1基因甲基化對提高結直腸癌甲基化暘性率的意義.方法 收集90例結直腸癌(結直腸癌組)和60例腺瘤性息肉(腺瘤組)患者及20例結直腸正常組織(正常對照組)標本,提取組織標本的DNA,採用甲基化特異性PCR(MSP)檢測Vimentin、sFRP1和HPP1基因甲基化狀態,分析其與結直腸癌的關繫及甲基化暘性率.結果 結直腸癌組Vimentin、sFRP1和HPP1基因甲基化率分彆為66.7％(60/90)、68.9％ (62/90)和72.2％(65/90);腺瘤組則分彆為53.3％(32/60)、55.0％( 33/60)和50.0％ (30/60);正常對照組分彆為0、0和5.0％(1/20).結直腸癌組3箇基因甲基化暘性率均高于腺瘤組和正常對照組(P＜0.05).3箇基因甲基化聯閤檢測診斷結直腸癌和腺瘤的暘性率分彆為93.3％(84/90)和76.7％(46/60),高于單箇基因檢測的甲基化暘性率(P＜0.05).3箇基因甲基化狀態與本組結直腸癌患者的性彆、年齡、腫瘤部位、淋巴結轉移、遠處轉移及TNM分期無關(P＞0.05).結論 Vimentin、sFRP1和HPP1基因啟動子甲基化水平在結直腸癌組織中升高,其聯閤檢測明顯提高瞭結直腸癌甲基化檢測暘性率,有可能成為結直腸癌早期診斷的甲基化檢測方案.
목적 탐토연합검측Vimentin、sFRP1화HPP1기인갑기화대제고결직장암갑기화양성솔적의의.방법 수집90례결직장암(결직장암조)화60례선류성식육(선류조)환자급20례결직장정상조직(정상대조조)표본,제취조직표본적DNA,채용갑기화특이성PCR(MSP)검측Vimentin、sFRP1화HPP1기인갑기화상태,분석기여결직장암적관계급갑기화양성솔.결과 결직장암조Vimentin、sFRP1화HPP1기인갑기화솔분별위66.7％(60/90)、68.9％ (62/90)화72.2％(65/90);선류조칙분별위53.3％(32/60)、55.0％( 33/60)화50.0％ (30/60);정상대조조분별위0、0화5.0％(1/20).결직장암조3개기인갑기화양성솔균고우선류조화정상대조조(P＜0.05).3개기인갑기화연합검측진단결직장암화선류적양성솔분별위93.3％(84/90)화76.7％(46/60),고우단개기인검측적갑기화양성솔(P＜0.05).3개기인갑기화상태여본조결직장암환자적성별、년령、종류부위、림파결전이、원처전이급TNM분기무관(P＞0.05).결론 Vimentin、sFRP1화HPP1기인계동자갑기화수평재결직장암조직중승고,기연합검측명현제고료결직장암갑기화검측양성솔,유가능성위결직장암조기진단적갑기화검측방안.
Objective To study whether combined detection of the methylation status of vimentin,sFRP1,and HPP1 gene can increase the positive methylation rate in colorectal cancer.Methods Tissue samples were collected from 90 patients with colorectal cancer,60 patients with adenomatous polyp,and 20 heathy controls.DNA was extracted and the methylation status of vimentin,sFRP1,and HPP1 gene was detected by Methylation-specific PCR (MSP).The relationship between clinicopathologic features of colorectal cancer and gene methylation was analyzed.Results The methylation rates of vimentin,sFRP1,and HPPI were 66.7％,68.9％,and 72.2％ in colorectal cancer,53.3％,55.0％,and 50.0％ in colorectal adenomas,and 0,0,and 5.0％ in healthy controls,respectively.The methylation of each of the three genes in colorectal cancer tissues was higher than colorectal adenomas and healthy controls (P＜0.05).The diagnostic sensitivity by combining three methylation markers was 93.3％ in colorectal cancer,76.7％ in colorectal adenomas,which was higher than the sensitivity using single gene testing (P＜0.05).No significant associations existed between the methylation status of the three genes and clinical characteristics including sex,age,tumor location,lymph node metastases,distant metastasis,and TNM stage (P＞0.05).Conclusions DNA methylation levels of vimentin,sFRP1 and HPP1 are significantly higher in colorectal cancer tissue.Combined detection significantly improves the positive rate of methylation,and may be used as early diagnosis method for colorectal cancer.
