CAJ | 학술논문

目的探讨超声击破微泡对犬睾丸及组织细胞的影响.方法 16只雄性成年犬的16个睾丸,随机分为3组,微泡(MB)组4个,治疗超声(TUS)组和微泡超声(MEUS)组各6个,依分组分别施以静脉注射微泡、超声治疗和微泡联合超声治疗后进行光镜和电镜检查.结果 MEUS组光镜下生精上皮层次明显减少,生精细胞排列紊乱,出现明显空泡,组织间隙微血管充血,管壁破损,红细胞溢出,部分生精小管内见红细胞;电镜下细胞间隙增宽,生精小管基膜局部断裂,支持细胞间的紧密连接消失.TUS组生精上皮层次稍减少,部分生精细胞出现空泡；基膜未见明显改变,组织间隙内偶见毛细血管充血,支持细胞间的紧密连接疏松.MB组未见明显变化.结论超声击破微泡可打开血睾屏障,为睾丸疾病靶向性治疗提供依据.
목적 탐토초성격파미포대견고환급조직세포적영향.방법 16지웅성성년견적16개고환,수궤분위3조,미포(MB)조4개,치료초성(TUS)조화미포초성(MEUS)조각6개,의분조분별시이정맥주사미포、초성치료화미포연합초성치료후진행광경화전경검사.결과 MEUS조광경하생정상피층차명현감소,생정세포배렬문란,출현명현공포,조직간극미혈관충혈,관벽파손,홍세포일출,부분생정소관내견홍세포;전경하세포간극증관,생정소관기막국부단렬,지지세포간적긴밀련접소실.TUS조생정상피층차초감소,부분생정세포출현공포；기막미견명현개변,조직간극내우견모세혈관충혈,지지세포간적긴밀련접소송.MB조미견명현변화.결론 초성격파미포가타개혈고병장,위고환질병파향성치료제공의거.
Objective To explore the effects on dogs' testis by microbubbles excitated by ultrasound.Methods Sixteen male dogs were divided into 3 groups randomly.Pulsed ultrasound irradiation and intravenous microbubbles injection were both applied in the microbubble enhanced ultrasound group (MEUS),pulsed ultrasound irradiation and intravenous microbubbles injection were individually applied in the ultrasound group (TUS) and the simple microbubbles group (MB).Results In MEUS group,the layers of seminiferous tubules decreased and the germ cells were arranged disorderly and bubbles appeared in the cells.The appearances of focal ultra-structural damage such as intercellular space widening,baseline broken and tight-connection of sustentacular cells disappearing were observed by transmission electron microscope,which were slightly or hardly demonstrated in other groups.Conclusions Microbubbles excitated by ultrasound can affect testicular germ cells and the integrity of bloold-testis harrier,which providing new train of thought of target treatments of testis diseases.