中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2008年
4期
469-470
,共2页
结直肠癌%血管内皮生长因子%转移
結直腸癌%血管內皮生長因子%轉移
결직장암%혈관내피생장인자%전이
Colorectal carcinoma%VEGF%Metastasis
目的 检测肿瘤内皮标记物(TEM-8)、血管皮皮生长因子(VEGF)-D在结、直肠癌中的表达及其意义.方法 用实时定量PCR方法分析30例结、直肠癌标本中TEM-8、VEGF-D的表达,并结合病理学分期,淋巴结转移等肿瘤特点进行分析.结果 VEGF-D在肿瘤组织中的表达较正常组织中升高(含量分别为0.006 72±0.003 05、0.000 41±0.000 23,P＜0.05),并与淋巴结转移相关(P＜0.05),而与肿瘤分期无明显相关性(P＞0.05),TEM-8在正常组织中基本无阳性表达,而在结、直肠癌组织中阳性表达率为90%,TEM-8与肿瘤分期(Dukes B,Dukes C样本中的含量分别为0.0278±0.0141、0.0375±0.0162,P＜0.05),淋巴结转移情况(在有淋巴结转移和无淋巴结转移的样本中的含量分别为0.0415±0.0137、0.021 30±0.005 84,P＜0.05).结论 TEM-8,VEGF-D均可作为结、直肠癌的标记物,并可反映淋巴结转移状况,TEM-8更可作为肿瘤特异性标记物.
目的 檢測腫瘤內皮標記物(TEM-8)、血管皮皮生長因子(VEGF)-D在結、直腸癌中的錶達及其意義.方法 用實時定量PCR方法分析30例結、直腸癌標本中TEM-8、VEGF-D的錶達,併結閤病理學分期,淋巴結轉移等腫瘤特點進行分析.結果 VEGF-D在腫瘤組織中的錶達較正常組織中升高(含量分彆為0.006 72±0.003 05、0.000 41±0.000 23,P＜0.05),併與淋巴結轉移相關(P＜0.05),而與腫瘤分期無明顯相關性(P＞0.05),TEM-8在正常組織中基本無暘性錶達,而在結、直腸癌組織中暘性錶達率為90%,TEM-8與腫瘤分期(Dukes B,Dukes C樣本中的含量分彆為0.0278±0.0141、0.0375±0.0162,P＜0.05),淋巴結轉移情況(在有淋巴結轉移和無淋巴結轉移的樣本中的含量分彆為0.0415±0.0137、0.021 30±0.005 84,P＜0.05).結論 TEM-8,VEGF-D均可作為結、直腸癌的標記物,併可反映淋巴結轉移狀況,TEM-8更可作為腫瘤特異性標記物.
목적 검측종류내피표기물(TEM-8)、혈관피피생장인자(VEGF)-D재결、직장암중적표체급기의의.방법 용실시정량PCR방법분석30례결、직장암표본중TEM-8、VEGF-D적표체,병결합병이학분기,림파결전이등종류특점진행분석.결과 VEGF-D재종류조직중적표체교정상조직중승고(함량분별위0.006 72±0.003 05、0.000 41±0.000 23,P＜0.05),병여림파결전이상관(P＜0.05),이여종류분기무명현상관성(P＞0.05),TEM-8재정상조직중기본무양성표체,이재결、직장암조직중양성표체솔위90%,TEM-8여종류분기(Dukes B,Dukes C양본중적함량분별위0.0278±0.0141、0.0375±0.0162,P＜0.05),림파결전이정황(재유림파결전이화무림파결전이적양본중적함량분별위0.0415±0.0137、0.021 30±0.005 84,P＜0.05).결론 TEM-8,VEGF-D균가작위결、직장암적표기물,병가반영림파결전이상황,TEM-8경가작위종류특이성표기물.
Objective To investigate the expression and significance of VEGF-D and TEM-8 in colorectal cancer.Methods Tumor tissue and adjacent normal mucosa were obtained from 30 colorectal cancer patients immediately after surgical resection.Levels of VEGF-D and TEM-8 were examined for each sample by using RT-PCR.Results VEGF-D levels were significantly higher in tumor tissues than in normal mucosa(0.006 72±0.003 05 vs 0.000 41±0.000 23,respectively,P＜0.05),and in the cases with regional lymphnode metastasis,VEGF-D levels were significantly higher than those without regional lymphnode metastasis(0.008 47±0.002 18 vs 0.004 81±0.001 08,respectively,P＜0.05),but no correlation was found between VEGF-D level and tumor stage(P＞0.05).TEM-8 level was correlated with both regional node metastasis and tumor stage(P＜0.05).Conclusion VEGF-D and TEM-8 can act as tumor markers for colorectals cancer,and show correlation with regional lymphnode metastasis.TEM-8 was proved to be an tumor specific marker,and may play an important role in the diagnosis,prognostic prediction,and treatment of colorectal cancer.
