CAJ | 학술논문

目的研究用纳米缓释技术处理的雷帕霉素(RPM-NP)对移植静脉狭窄的防治作用.方法选取家兔40只,建立兔颈外静脉间位移植至颈总动脉的移植静脉模型,按处理方式不同,分为4组:药物纳米局部干预组(RPM-NP局部处理颈外静脉,n=10,A组)、药物纳米全身干预组(RPMNP周围静脉滴注,n=10,B组)、空白纳米局部干预组(空白纳米粒局部处理颈外静脉,n=10,C组)和未干预组(不做上述处理,n=10,D组).术后28 d,切取各组移植静脉和对侧正常静脉,病理学检测移植静脉内膜厚度、内径、内膜/中膜厚度比以及胶原容积指数.结果移植静脉的组织病理学检测结果表明,A、B、C、D组移植静脉和对侧正常静脉的内膜/中膜厚度比分别为0.26±0.02、0.73±0.05、0.71 ±0.04、0.69±0.03、0.24±0.01,胶原容积指数比分别为0.24±0.03、0.56±0.06、0.53±0.07、0.49±0.08、0.21 ±0.01.B、C、D组的组织病理学(移植静脉内膜厚度、内径、内膜/中膜厚度比以及胶原容积指数)与对侧正常静脉组比较,均出现明显的病理学改变,差异均有统计学意义(均P＜0.05)；3组之间移植静脉的组织病理学结果比较,差异均无统计学意义(均P＞0.05).而A组移植静脉的组织病理学结果与对侧正常静脉比较,差异无统计学意义(P＞0.05)；与其他3组比较,差异有统计学意义(P＜0.05).结论采用纳米缓释技术处理的雷帕霉素,对离体的移植静脉行管腔内局部处理,能抑制移植静脉内膜增生,防治移植静脉狭窄.
목적 연구용납미완석기술처리적뢰파매소(RPM-NP)대이식정맥협착적방치작용.방법 선취가토40지,건립토경외정맥간위이식지경총동맥적이식정맥모형,안처리방식불동,분위4조:약물납미국부간예조(RPM-NP국부처리경외정맥,n=10,A조)、약물납미전신간예조(RPMNP주위정맥적주,n=10,B조)、공백납미국부간예조(공백납미립국부처리경외정맥,n=10,C조)화미간예조(불주상술처리,n=10,D조).술후28 d,절취각조이식정맥화대측정상정맥,병이학검측이식정맥내막후도、내경、내막/중막후도비이급효원용적지수.결과 이식정맥적조직병이학검측결과표명,A、B、C、D조이식정맥화대측정상정맥적내막/중막후도비분별위0.26±0.02、0.73±0.05、0.71 ±0.04、0.69±0.03、0.24±0.01,효원용적지수비분별위0.24±0.03、0.56±0.06、0.53±0.07、0.49±0.08、0.21 ±0.01.B、C、D조적조직병이학(이식정맥내막후도、내경、내막/중막후도비이급효원용적지수)여대측정상정맥조비교,균출현명현적병이학개변,차이균유통계학의의(균P＜0.05)；3조지간이식정맥적조직병이학결과비교,차이균무통계학의의(균P＞0.05).이A조이식정맥적조직병이학결과여대측정상정맥비교,차이무통계학의의(P＞0.05)；여기타3조비교,차이유통계학의의(P＜0.05).결론 채용납미완석기술처리적뢰파매소,대리체적이식정맥행관강내국부처리,능억제이식정맥내막증생,방치이식정맥협착.
Objective To explore the effects of pretreatment of carbopol-encapsulated rapamycinloaded nanoparticles(RPM-NP)on vein graft stenosis in a rabbit vein graft model.Methods A segment of common carotid artery was replaced with a segment of external jugular vein in 40 rabbits.They were separated into four treatment groups,i.e.Group A:vein grafts were pretreated with intraluminal RPM-NP perfusion; Group B:peripheral venous veins were injected with RPM-NP; Group C:vein grafts received an equivalent perfusion of empty vehicle; Group D:vein grafts received no treatment.At Day 28 postoperation,the grafts and normal veins were harvested for histological examinations to analyze the indicators of intimal thickness,internal diameter,intimal/media thickness ratio and collagen volume index.Results At Day 28 post-operation,the intimal/media thickness ratios were 0.26 ± 0.02,0.73 ± 0.05,0.71 ± 0.04,0.69 ±0.03 and 0.24 ±0.01 in Groups A,B,C and D and the normal vein; the collagen volume index 0.24±0.03,0.56±0.06,0.53 ±0.07,0.49 ±0.08 and 0.21 ±0.01 respectively.Compared with the normal veins,the pathological indicators of vein graft intimal thickness,internal diameter,intimal/media thickness ratio and collagen volume index had significant differences in Groups B,C and D(all P ＜0.05).But there were no significant differences among 3 groups(all P ＞0.05).Compared with the normal vein,the parameters of vein graft intimal thickness,internal diameter,intimal/media thickness ratio and collagen volume index had no significant difference in Group A(all P ＞0.05).But as compared with other groups,these indicators had statistical significant difference in Group A(all P ＜ 0.05).Conclusion The local pretreatment of isolated vein with rapamycin nanoparticles may inhibit neointimal hyperplasia and prevent effectively vein graft stenosis.