中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2009年
8期
1059-1061
,共3页
祝恒成%刘修恒%陶涛%高瑞辉%但超
祝恆成%劉脩恆%陶濤%高瑞輝%但超
축항성%류수항%도도%고서휘%단초
树突状细胞%IκBα突变体%基因%肾移植
樹突狀細胞%IκBα突變體%基因%腎移植
수돌상세포%IκBα돌변체%기인%신이식
Dendritic cells%IκBα mutant%C,ene%Renal transplantation
目的 观察IκBα突变体基因修饰的供体树突状细胞(IκBαM-Dc)对大鼠移植肾存活时间的影响.方法 利用重组腺病毒载体将IκBαM基因转染WF大鼠骨髓DC,流式细胞仪检测DC共刺激分子CD80、CD86表达.以wF大鼠为供体,Lewis大鼠为受体,行同种肾移植.术前7 d,受体输注供体IκBαM-DC作为IκBαM组,未输注IκBαM-DC的受体作为对照组,观察移植肾存活时间和术后肾功能变化.术后第14天检测受体T细胞对供体成熟DC及第三方大鼠成熟DC的反应性.结果 IκBαM明显抑制DC共刺激分子CD80、CD86表达.IκBαM组受体鼠移植肾存活时间为(32.5±7.8)d,较对照组移植肾存活时间(8.5±1.7)d明显延长(P<0.01);而其术后7 d血清肌酐为(57.3±8.2)μmol/L,较对照组血清肌酐(498.0±46.3)μmol/L明显减低.肾移植术后,IκBαM组受体T细胞对供体成熟DC反应明显低于对照组,而对第三方大鼠成熟DC的反应性与对照组比较,差异无统计学意义(P>0.05).结论 表达IκBα突变体的供体树突状细胞能诱导受体大鼠T细胞对供体抗原的免疫低反应,显著延长大鼠移植肾存活时间.
目的 觀察IκBα突變體基因脩飾的供體樹突狀細胞(IκBαM-Dc)對大鼠移植腎存活時間的影響.方法 利用重組腺病毒載體將IκBαM基因轉染WF大鼠骨髓DC,流式細胞儀檢測DC共刺激分子CD80、CD86錶達.以wF大鼠為供體,Lewis大鼠為受體,行同種腎移植.術前7 d,受體輸註供體IκBαM-DC作為IκBαM組,未輸註IκBαM-DC的受體作為對照組,觀察移植腎存活時間和術後腎功能變化.術後第14天檢測受體T細胞對供體成熟DC及第三方大鼠成熟DC的反應性.結果 IκBαM明顯抑製DC共刺激分子CD80、CD86錶達.IκBαM組受體鼠移植腎存活時間為(32.5±7.8)d,較對照組移植腎存活時間(8.5±1.7)d明顯延長(P<0.01);而其術後7 d血清肌酐為(57.3±8.2)μmol/L,較對照組血清肌酐(498.0±46.3)μmol/L明顯減低.腎移植術後,IκBαM組受體T細胞對供體成熟DC反應明顯低于對照組,而對第三方大鼠成熟DC的反應性與對照組比較,差異無統計學意義(P>0.05).結論 錶達IκBα突變體的供體樹突狀細胞能誘導受體大鼠T細胞對供體抗原的免疫低反應,顯著延長大鼠移植腎存活時間.
목적 관찰IκBα돌변체기인수식적공체수돌상세포(IκBαM-Dc)대대서이식신존활시간적영향.방법 이용중조선병독재체장IκBαM기인전염WF대서골수DC,류식세포의검측DC공자격분자CD80、CD86표체.이wF대서위공체,Lewis대서위수체,행동충신이식.술전7 d,수체수주공체IκBαM-DC작위IκBαM조,미수주IκBαM-DC적수체작위대조조,관찰이식신존활시간화술후신공능변화.술후제14천검측수체T세포대공체성숙DC급제삼방대서성숙DC적반응성.결과 IκBαM명현억제DC공자격분자CD80、CD86표체.IκBαM조수체서이식신존활시간위(32.5±7.8)d,교대조조이식신존활시간(8.5±1.7)d명현연장(P<0.01);이기술후7 d혈청기항위(57.3±8.2)μmol/L,교대조조혈청기항(498.0±46.3)μmol/L명현감저.신이식술후,IκBαM조수체T세포대공체성숙DC반응명현저우대조조,이대제삼방대서성숙DC적반응성여대조조비교,차이무통계학의의(P>0.05).결론 표체IκBα돌변체적공체수돌상세포능유도수체대서T세포대공체항원적면역저반응,현저연장대서이식신존활시간.
Objective To study the effects of donor dendritic cells (DCs) genetically engineered to express IκBα mutant (IKBctM-DC) on renal allografts survival in rats.Methods DCs were prepared from WF rat bone marrow cells and modified by IκBαM gene with adenovirus vector, and the cell-surface expression of costimulatory molecules (CD80, CD86) was analyzed by flow cytometry.Allograft kidney transplantation animal models were established by using WF rats as donors, and Lewis rats as recipients.The rats in IκBαM model group were injected with IκBαM-DCs from donors through tail vein at the 7th day before allograft kidney transplantation, and those without injection served as the controls group.The allograft survival time and the allograft function after transplantation between the two groups were compared.T cell reactions of the recipients to mature DCs from donors and those from the third party rats were assayed by using MTT on the postoperative 14th day.Results IκBαM suppressed DC-surface expression of costimulatory molecules (CD80 and CD86).The allograf t survival time in IκBαM model group was longer than in controls significantly [(32.5 ± 7.8) days vs (8.5 ± 1.7) days, P < 0.01].The serum creatinine in transfection group at the postoperative day 7 was lower than in controls [(57.3 ± 8.2) vs (498.0 ± 46.3) μmol/L,P <0.01].On the day14,the T cells of recipients in IκBαM model group showed significant hyporesponsiveness to mature DCs from donors, but remained normal reactions to mature DCs from the third party rats.Conclusion Donor DCs genetically engineered to express IκBα mutant can suppress the immune response of recipients'T lymphocytes to donor antigen specifically, and prolong the survival of rehal aUografts significantly.