CAJ | 학술논문

以蝴蝶兰(Phalaenopsis)花梗腋芽为外植体,研究出一种高效、简便的诱导原球茎(Protocorm)的方法.在不含其他有机添加物,只添加BA (3 mg/L)+NAA (0.1 mg/L)的MS培养基中,原球茎诱导效率达80%. 相同培养基继代,4周内即可发育成幼苗.在含有IAA(0.1 mg/L)的MS培养基中,40%的幼苗可以诱导生根,并移植成功.由于此方法简便、经济有效,可应用于工厂化生产,而且获得的优质原球茎也为进一步的遗传改良提供材料.
이호접란(Phalaenopsis)화경액아위외식체,연구출일충고효、간편적유도원구경(Protocorm)적방법.재불함기타유궤첨가물,지첨가BA (3 mg/L)+NAA (0.1 mg/L)적MS배양기중,원구경유도효솔체80%. 상동배양기계대,4주내즉가발육성유묘.재함유IAA(0.1 mg/L)적MS배양기중,40%적유묘가이유도생근,병이식성공.유우차방법간편、경제유효,가응용우공엄화생산,이차획득적우질원구경야위진일보적유전개량제공재료.
An efficient and simple method of high frequency protocorm regeneration from pedicel axillary bud of Phalaenopsis is described. Pedicel axillary buds were cultured on Murashige and Skoog's basal medium (MS) with combinations of 6-benzylaminopurine (BA) (2-3 mg/L) and α-naphthaleneacetic acid (NAA) (0.1-0.5 mg/L). Medium supplemented with 3 mg/L BA in combination with 0.1 mg/LNAA produced the best response in protocorm occurrence(80%) without addition of any organic material. On subculture to the same medium, protocorm produced the shoots within 4 weeks of culture and well developing shoots were obtained separately. 40% of developed shoots produced root on medium supplemented with 0.1 mg/L IAA and the plants were transferred to moss for growth. Because of the simplicity for culture medium ingredient, it is a reliable protocol for plant regeneration of Phalaenopsis factory production in developing country and is also useful to genetic improvement programs.