生理学报
生理學報
생이학보
ACTA PHYSIOLOGICA SINICA
2007年
1期
58-62
,共5页
高莉%沈金宝%孙捷%单保恩
高莉%瀋金寶%孫捷%單保恩
고리%침금보%손첩%단보은
雷氏大疣蛛毒液%HepG2细胞%细胞周期%p21基因%p21蛋白
雷氏大疣蛛毒液%HepG2細胞%細胞週期%p21基因%p21蛋白
뢰씨대우주독액%HepG2세포%세포주기%p21기인%p21단백
the venom of the spider Macrothele raveni%HepG2 cells%cell cycle%p21 gene%p21 protein
本文研究了雷氏大疣蛛毒液对人肝癌细胞株HepG2增殖抑制作用及其分子机制.采用XTT法观察到雷氏大疣蛛毒液剂量依赖抑制HepG2细胞增殖;流式细胞仪检测发现,经过雷氏大疣蛛毒液作用的HepG2细胞周期发生明显的选择性改变;RT-PCR方法检测到p21基因表达增强;Western blot检测发现,p21蛋白表达增加.结果提示,雷氏大疣蛛毒液抑制人肝癌细胞HepG2增殖的可能机制之一是使p21基因和蛋白表达增加,G2/M细胞周期被阻滞,从而诱导细胞凋亡.
本文研究瞭雷氏大疣蛛毒液對人肝癌細胞株HepG2增殖抑製作用及其分子機製.採用XTT法觀察到雷氏大疣蛛毒液劑量依賴抑製HepG2細胞增殖;流式細胞儀檢測髮現,經過雷氏大疣蛛毒液作用的HepG2細胞週期髮生明顯的選擇性改變;RT-PCR方法檢測到p21基因錶達增彊;Western blot檢測髮現,p21蛋白錶達增加.結果提示,雷氏大疣蛛毒液抑製人肝癌細胞HepG2增殖的可能機製之一是使p21基因和蛋白錶達增加,G2/M細胞週期被阻滯,從而誘導細胞凋亡.
본문연구료뢰씨대우주독액대인간암세포주HepG2증식억제작용급기분자궤제.채용XTT법관찰도뢰씨대우주독액제량의뢰억제HepG2세포증식;류식세포의검측발현,경과뢰씨대우주독액작용적HepG2세포주기발생명현적선택성개변;RT-PCR방법검측도p21기인표체증강;Western blot검측발현,p21단백표체증가.결과제시,뢰씨대우주독액억제인간암세포HepG2증식적가능궤제지일시사p21기인화단백표체증가,G2/M세포주기피조체,종이유도세포조망.
This paper focuses on the effect of the venom of the spider Macrothele raveni on the proliferation of human hepatocelluar carcinoma cell line HepG2 and the related molecular mechanism. XTT test showed that the proliferation of HepG2 cells in vitro was inhibited by the spider venom (P<0.05) in a concentration-dependent manner. By using flow cytometry, it was found that the spider venom caused selective G2/M cell cycle arrest in HepG2 cells. RT-PCR and Western blot indicated the expressions of p21 mRNA and protein in HepG2 cells were obviously up-regulated by the spider venom. The venom of the spider Macrothele raveni inhibited the proliferation of HepG2 cells. These results suggest that the possible mechanism of the spider venom is to activate the expressions of p21 gene and protein and to cause selective cell cycle arrest at G2/M phase, leading to HepG2 cell apoptosis.