CAJ | 학술논문

背景:新型生物材料的孔径形状、大小以及孔隙率对引导细胞的附着、浸润和生长具有重要作用.目的:初步评价新型纳米仿生骨植入材料3-羟基丁酸-co-3-羟基戊酸共聚物/溶胶-凝胶生物活性玻璃[poly(3-hydroxybutyrateco-3-hydroxyvalerate)/sol gel bioactive glass,PHBV/SGBG]的生物相容性.设计、时间及地点:细胞-材料学实验,于2006-03/09 在中山大学附属第三医院中心实验室完成.材料:PHBV/SGBG由华南理工大学材料学研究所提供,骨髓基质细胞为课题组前期制备.方法:确定PHBV/SGBG浸提液的标准浓度为培养液与材料表面积之比10 mL/cm2,将PHBV/SGBG浸入完全培养液中,于37℃、体积分数为5%的CO2饱和湿度培养箱中浸提两三天,吸出浸提液,无菌封存备用.同法制备8倍、4倍、2倍、1倍、0.5倍、0.25倍、0.125倍标准浓度的PHBV/SGBG浸提液.主要观察指标:扫描电镜观察PHBV/SGBG超微结构,采用常规称量法计算PHBV/SGBG孔隙率,MTT法评价材料的毒性分级,通过直接接触培养法观察骨髓基质细胞与PHBV/SGBG材料的生物相容性.结果:电镜下 PHBV/SGBG材料为疏松多孔结构,孔隙相互联通,其中可见纳米级的SGBG颗粒镶嵌或包裹在PHBV基体孔壁上,孔隙率＞90%.培养第1,3,5天各浓度PHBV/SGBG浸提液组毒性分级均在0～1级.骨髓基质细胞可紧密附着于PHBV/SGBG材料表面,黏附生长,并有突起向PHBV/SGBG材料的连通微孔内伸展.结论:新型纳米仿牛骨植入材料PHBV/SGBG对骨髓基质细胞无毒性作用,具有优良的细胞亲和性,可能与材料的泡沫多孔状结构有关.
배경:신형생물재료적공경형상、대소이급공극솔대인도세포적부착、침윤화생장구유중요작용.목적:초보평개신형납미방생골식입재료3-간기정산-co-3-간기무산공취물/용효-응효생물활성파리[poly(3-hydroxybutyrateco-3-hydroxyvalerate)/sol gel bioactive glass,PHBV/SGBG]적생물상용성.설계、시간급지점:세포-재료학실험,우2006-03/09 재중산대학부속제삼의원중심실험실완성.재료:PHBV/SGBG유화남리공대학재료학연구소제공,골수기질세포위과제조전기제비.방법:학정PHBV/SGBG침제액적표준농도위배양액여재료표면적지비10 mL/cm2,장PHBV/SGBG침입완전배양액중,우37℃、체적분수위5%적CO2포화습도배양상중침제량삼천,흡출침제액,무균봉존비용.동법제비8배、4배、2배、1배、0.5배、0.25배、0.125배표준농도적PHBV/SGBG침제액.주요관찰지표:소묘전경관찰PHBV/SGBG초미결구,채용상규칭량법계산PHBV/SGBG공극솔,MTT법평개재료적독성분급,통과직접접촉배양법관찰골수기질세포여PHBV/SGBG재료적생물상용성.결과:전경하 PHBV/SGBG재료위소송다공결구,공극상호련통,기중가견납미급적SGBG과립양감혹포과재PHBV기체공벽상,공극솔＞90%.배양제1,3,5천각농도PHBV/SGBG침제액조독성분급균재0～1급.골수기질세포가긴밀부착우PHBV/SGBG재료표면,점부생장,병유돌기향PHBV/SGBG재료적련통미공내신전.결론:신형납미방우골식입재료PHBV/SGBG대골수기질세포무독성작용,구유우량적세포친화성,가능여재료적포말다공상결구유관.
BACKGROUND:Pore diameter,size and porosity of biomaterials are important for cell attachment,infiltration and growth.OBJECTIVE:To primarily evaluate the biocompatibility of novel bionic scaffold of poly(3-hydroxybutyrate-co-3-hydroxyvalerate)/sol gel bioactive glass (PHBV/SGBG) with nanostructure in vitro.DESIGN,TIME AND SETTING:Cell-material experiment was performed at the Central Laboratory of Third Hospital of Sun Yat-sen University from March to September 2006.MATERIALS:PHBV/SGBG was provided by the Institute of Materials Science and Engineering,South China University of Technology;bone marrow stroma cells (MSCs) were prepared by our team.METHODS:The standard concentration of PHBV/SGBG extracting solution should be 10 mL/ cm2 of the ratio of culture solution to material surface.PHBV/SGBG was immersed into the complete culture solution and incubated in 5% CO2 at 37 ℃ for 2-3 days.The extracting solution was drawn and stored under sterile condition.In addition,PHBV/SGBG extracting solution of 8,4,2,1,0.5,0.25 and 0.125 times of standard concentration was prepared.MAIN OUTCOME MEASURES:PHBV/SGBG ultrastructure was observed by scanning electron microscopy;PHBV/SGBG porosity was measured by routine measurement.MTT methods were used as a quantitative assessment for cytotoxicity of the biomaterials.Adherence and spreading of MSCs on the surface of specimen was observed using direct contact cultivation.RESULTS:The PHBV/SGBG was porous,with connecting pores under electron microscopy.Nanometer SGBG particles were imbedded or encapsulated in pore wall of PHBV,with the porosity ＞90%.The toxicity gradation of the novel bionic scaffold ranked from grade 0 to 1 at 1,3,5 days of culture.MSCs slightly attached and grew on the surface of the biomaterials,and proliferated rapidly.Obvious cell processes stretched into the micro-pores structure.CONCLUSION:The novel bionic scaffold of PHBV/SGBG has excellent cellular affinity,possibly due to the porous structure,with no cytotoxicity to MSCs.