中山大学学报（医学科学版）
中山大學學報（醫學科學版）
중산대학학보（의학과학판）
JOURNAL OF SUN YAT-SEN UNIVERSITY(MEDICAL SCIENCES)
2010年
2期
253-257
,共5页
肖琳%何丹%丁运刚%冯智英%邵春奎
肖琳%何丹%丁運剛%馮智英%邵春奎
초림%하단%정운강%풍지영%소춘규
EB病毒相关胃癌%肿瘤浸润性T淋巴细胞%肿瘤浸润性树突细胞
EB病毒相關胃癌%腫瘤浸潤性T淋巴細胞%腫瘤浸潤性樹突細胞
EB병독상관위암%종류침윤성T림파세포%종류침윤성수돌세포
EBV-associated gastric carcinoma%tumor infiltrating T lymphocytes%tumor infiltrating dendritic cells
[目的]探讨肿瘤浸润树突状细胞(TIDC)及肿瘤浸润T淋巴细胞(TIL-T)在EBV相关胃癌(EBVaGC)中的肿瘤免疫作用及其临床意义.[方法]采用免疫组织化学方法检测49例EBVaGC和20例EBV阴性胃癌(EBVnGC)中TIDC及TIL-T的浸润程度及亚型,并分析其与EBVaGC的临床病理特征的关系.[结果]TIDC在49例EBVaGC中有29例(59.2%)呈轻度浸润,20例(40.8%)为显著浸润,而EBVnGC中则有18例(90%)为轻度浸润,2例(10%)为显著浸润.经统计学分析,TIDC在EBVaGC显著浸润率高于EBVnGC(P=0.013).TIL-T在EBVaGC的数量较EBVnGC多(P=0.017),且多数为CD8~+ 细胞毒性T细胞(CTL):EBVaGC中TIL-T与TIDC数量呈正相关(r=0.386,n=49,P=0.006).TIL-T浸润程度与淋巴结转移呈负相关(P＜0.001),而TIDC浸润程度与临床病理特征无关.[结论]TIDC及TIL-T可能在EBVaGC肿瘤免疫反应中起着重要的作用,TIDC可能对于TIL-T具有趋化作用.
[目的]探討腫瘤浸潤樹突狀細胞(TIDC)及腫瘤浸潤T淋巴細胞(TIL-T)在EBV相關胃癌(EBVaGC)中的腫瘤免疫作用及其臨床意義.[方法]採用免疫組織化學方法檢測49例EBVaGC和20例EBV陰性胃癌(EBVnGC)中TIDC及TIL-T的浸潤程度及亞型,併分析其與EBVaGC的臨床病理特徵的關繫.[結果]TIDC在49例EBVaGC中有29例(59.2%)呈輕度浸潤,20例(40.8%)為顯著浸潤,而EBVnGC中則有18例(90%)為輕度浸潤,2例(10%)為顯著浸潤.經統計學分析,TIDC在EBVaGC顯著浸潤率高于EBVnGC(P=0.013).TIL-T在EBVaGC的數量較EBVnGC多(P=0.017),且多數為CD8~+ 細胞毒性T細胞(CTL):EBVaGC中TIL-T與TIDC數量呈正相關(r=0.386,n=49,P=0.006).TIL-T浸潤程度與淋巴結轉移呈負相關(P＜0.001),而TIDC浸潤程度與臨床病理特徵無關.[結論]TIDC及TIL-T可能在EBVaGC腫瘤免疫反應中起著重要的作用,TIDC可能對于TIL-T具有趨化作用.
[목적]탐토종류침윤수돌상세포(TIDC)급종류침윤T림파세포(TIL-T)재EBV상관위암(EBVaGC)중적종류면역작용급기림상의의.[방법]채용면역조직화학방법검측49례EBVaGC화20례EBV음성위암(EBVnGC)중TIDC급TIL-T적침윤정도급아형,병분석기여EBVaGC적림상병리특정적관계.[결과]TIDC재49례EBVaGC중유29례(59.2%)정경도침윤,20례(40.8%)위현저침윤,이EBVnGC중칙유18례(90%)위경도침윤,2례(10%)위현저침윤.경통계학분석,TIDC재EBVaGC현저침윤솔고우EBVnGC(P=0.013).TIL-T재EBVaGC적수량교EBVnGC다(P=0.017),차다수위CD8~+ 세포독성T세포(CTL):EBVaGC중TIL-T여TIDC수량정정상관(r=0.386,n=49,P=0.006).TIL-T침윤정도여림파결전이정부상관(P＜0.001),이TIDC침윤정도여림상병리특정무관.[결론]TIDC급TIL-T가능재EBVaGC종류면역반응중기착중요적작용,TIDC가능대우TIL-T구유추화작용.
[Objective]To explore the roles of TIDC(tumor infiltrating dendritic cell)and TIL-T(tumor infiltrating T lymphocyte)in tumor immunity of EBV-associated gastric carcinoma(EBVaGC)and their clinical significance.[Methods]TIDC and TIL-T in EBVaGC(n=49)and EBVnGC(EBV-negative gastric carcinoma)(n=20)were detected by immunohistochemistry method and then their subtypes and their relationships with clinical and pathological factors were analyzed.[Results]Mild infiltration was detected in 29 of 49(59.2%)EBVaGCs and 18 of 20(90%)EBVnGCs,while marked infiltration of TIDC was detected in 20 of 49(40.8%)EBVaGCs and 2 of 20(10%)EBVnGCs.And the difference between the two groups was significant (P=0.013).The number of TIL-T in EBVaGC was hisher than that in EBVnGC(P=0.017),and most of them were CTLs.The number of TIL-T was positively correlated with that of TIDC(r=0.386,n=49,P=0.006).The degree of TIL-T infiltration in EBVaGC had negative relationship with the lymph node metastasis(P＜0.001),while TIDC had no relationship with the clinical features.[Conclusion]TIDC and TIL-T may play important roles in the tumor immunity of EBVaGC;TIDC may contribute to the TIL-T recruitment in EBVaGC.
