CAJ | 학술논문

目的了解尖吻腹蛇舌状虫若虫扫描与透射电镜超微结构.方法将尖吻腹蛇舌状虫若虫经常规方法环氧树脂包埋,再经超薄切片,双重染色后分别置于扫描电镜与透射电镜下进行观察.结果扫描电镜下观察若虫.口边缘偶见感觉器与乳突,并发现口内缘有皱褶,口两侧各有一对弯钩;虫体口和对钩外的体表上布满大小不同似吸盘样感觉器;腹环之间体表为波纹皱褶,腹环末节腹面可见肉芽状肛门开口.透射电镜下观察发现若虫皮层可分为外皮层、透明层、内皮层、均质层、髓质层、纹状层及基板;外皮层外侧布满颗粒细微的绒毛,髓质层内有大小不均致密颗粒,堆聚呈不同形状,并有内质网和微管通向均质层,髓质层纹状层走向呈波浪形纹;皮层下的皮质细胞在与基板连接处可见许多微管开口于基板,通向纹状层,胞质内充满大量线粒体.结论若虫口孔两侧的2对口钩及体表布满大小不同似吸盘样感觉器,虫体体表呈波纹皱褶状,虫体皮层下的细胞核周边有较多内质网、糖原颗粒及线粒体,通过许多微管开口于基板,通向皮层.
목적 료해첨문복사설상충약충소묘여투사전경초미결구.방법 장첨문복사설상충약충경상규방법배양수지포매,재경초박절편,쌍중염색후분별치우소묘전경여투사전경하진행관찰.결과 소묘전경하관찰약충.구변연우견감각기여유돌,병발현구내연유추습,구량측각유일대만구;충체구화대구외적체표상포만대소불동사흡반양감각기;복배지간체표위파문추습,복배말절복면가견육아상항문개구.투사전경하관찰발현약충피층가분위외피층、투명층、내피층、균질층、수질층、문상층급기판;외피층외측포만과립세미적융모,수질층내유대소불균치밀과립,퇴취정불동형상,병유내질망화미관통향균질층,수질층문상층주향정파랑형문;피층하적피질세포재여기판련접처가견허다미관개구우기판,통향문상층,포질내충만대량선립체.결론 약충구공량측적2대구구급체표포만대소불동사흡반양감각기,충체체표정파문추습상,충체피층하적세포핵주변유교다내질망、당원과립급선립체,통과허다미관개구우기판,통향피층.
Objective To observe the ultrastructure of nymphal Armillifer agkistrodontis by scanning and transmission electron microscope. Methods Nymphal A.agkistrodontis were collected from abdominal cavity of a mouse inoculated with ova of A.agkistrodontis from a snake in our laboratory.A.agkistrodontis nymphal were embedded in epoxy resin according to the routine method.The sample was cut into thin sections with a LKB super slicer,double stained and viewed under scanning electron microscope and transmissionelectron microscope. Results The outer shape, number of abdominal annuli.size and locality of the mouth were observed.Sense organ and papillae around the mouth of nymphal A.agkistrodontis were occasionally seen under scanning electron microscope.Folds were seen in inner margin of the mouth with a pair of curved hooks on both sides of it.Sucker-like sense organs of different sizes were fully distributed on the body surface.Waved folds were seen between the abdominal annuli of the body surface.Granulation-like anus opening was seen at the end of abdominal annuli.The cortical laver is consisted of outer cortex,lucidity layer,inner cortex,homogenous laver,medulla,striated layer and base plate observed under transmission electron microscope.Dense granules of different size were presented in the medulla forming different shapes with endoplasmic reticulum and canaliculus leading to homogenous layer.Medullary layer and striated laver were shown as wave shape.Below cortex cell of cortical layer many canaliculi were seen on the base plate.In cvtoplasm of the striated laver a large number of mitochondria could be seen. Conclusion The ultrastructure of nvmphal A.agkistrodontis was observed with scanning and transmission electron microscopy.Two pairs of hooks of mouth aperture and different sizes of sucker-like sense organ fully distributed on the body surface.Under worm cortical layer,around the nucleolus,there are many endoplasm webs,sugar granule and mitochondria by many canaliculi are opening on the base plate to cortical layer.