中华胰腺病杂志
中華胰腺病雜誌
중화이선병잡지
CHINESE JOURNAL OF PANCREATOLOGY
2011年
2期
89-91
,共3页
胰腺肿瘤%载脂蛋白E类%补体C4b%转移
胰腺腫瘤%載脂蛋白E類%補體C4b%轉移
이선종류%재지단백E류%보체C4b%전이
Pancreatic neoplasms%Apolipoproteins E%Complement C4b%Metastasis
目的 检测载脂蛋白E(ApoE)和补体C4b1在人胰腺癌组织中的表达,探讨其意义.方法 应用免疫组化法检测38例胰腺癌和相应癌旁正常胰腺组织ApoE、C4b1蛋白表达;应用RT-PCR法检测20例胰腺癌和相应癌旁正常胰腺组织ApoE、C4b1 mRNA表达.分析ApoE、C4b1的表达与胰腺癌生物特征的相关性.结果 胰腺癌组织ApoE、C4b1蛋白表达阳性率分别为86.8%(33/38)和76.3%(29/38),显著高于癌旁正常胰腺组织的42.1%(16/38)和26.3%(10/38)(P值均＜0.01);有淋巴结转移的胰腺癌组织ApoE、C4b1蛋白表达阳性率分别为78.3%(18/23)和73.9%(17/23),明显高于无转移者的33.3%(5/15)和40.0%(6/15)(P值均＜0.05).胰腺癌ApoE、C4b1 mRNA表达量分别为4.83±0.65、7.94±0.95,明显高于癌旁正常胰腺组织的1.78±0.74、1.22±0.57(P值均＜0.01);有淋巴结转移的胰腺癌组织ApoE、C4b1 mRNA的表达量为5.05±0.71、8.24±1.07,显著高于无转移者的4.42±0.25、7.39±0.15(P值均＜0.05).结论 ApoE、C4b1在胰腺癌组织中高表达,并与胰腺癌淋巴结转移相关.
目的 檢測載脂蛋白E(ApoE)和補體C4b1在人胰腺癌組織中的錶達,探討其意義.方法 應用免疫組化法檢測38例胰腺癌和相應癌徬正常胰腺組織ApoE、C4b1蛋白錶達;應用RT-PCR法檢測20例胰腺癌和相應癌徬正常胰腺組織ApoE、C4b1 mRNA錶達.分析ApoE、C4b1的錶達與胰腺癌生物特徵的相關性.結果 胰腺癌組織ApoE、C4b1蛋白錶達暘性率分彆為86.8%(33/38)和76.3%(29/38),顯著高于癌徬正常胰腺組織的42.1%(16/38)和26.3%(10/38)(P值均＜0.01);有淋巴結轉移的胰腺癌組織ApoE、C4b1蛋白錶達暘性率分彆為78.3%(18/23)和73.9%(17/23),明顯高于無轉移者的33.3%(5/15)和40.0%(6/15)(P值均＜0.05).胰腺癌ApoE、C4b1 mRNA錶達量分彆為4.83±0.65、7.94±0.95,明顯高于癌徬正常胰腺組織的1.78±0.74、1.22±0.57(P值均＜0.01);有淋巴結轉移的胰腺癌組織ApoE、C4b1 mRNA的錶達量為5.05±0.71、8.24±1.07,顯著高于無轉移者的4.42±0.25、7.39±0.15(P值均＜0.05).結論 ApoE、C4b1在胰腺癌組織中高錶達,併與胰腺癌淋巴結轉移相關.
목적 검측재지단백E(ApoE)화보체C4b1재인이선암조직중적표체,탐토기의의.방법 응용면역조화법검측38례이선암화상응암방정상이선조직ApoE、C4b1단백표체;응용RT-PCR법검측20례이선암화상응암방정상이선조직ApoE、C4b1 mRNA표체.분석ApoE、C4b1적표체여이선암생물특정적상관성.결과 이선암조직ApoE、C4b1단백표체양성솔분별위86.8%(33/38)화76.3%(29/38),현저고우암방정상이선조직적42.1%(16/38)화26.3%(10/38)(P치균＜0.01);유림파결전이적이선암조직ApoE、C4b1단백표체양성솔분별위78.3%(18/23)화73.9%(17/23),명현고우무전이자적33.3%(5/15)화40.0%(6/15)(P치균＜0.05).이선암ApoE、C4b1 mRNA표체량분별위4.83±0.65、7.94±0.95,명현고우암방정상이선조직적1.78±0.74、1.22±0.57(P치균＜0.01);유림파결전이적이선암조직ApoE、C4b1 mRNA적표체량위5.05±0.71、8.24±1.07,현저고우무전이자적4.42±0.25、7.39±0.15(P치균＜0.05).결론 ApoE、C4b1재이선암조직중고표체,병여이선암림파결전이상관.
Objective To investigate the expression of apolipoprotein E (ApoE) and complement C4b1 in pancreatic carcinoma and study its significance. Methods Immunohistochemistry was used to detect the expression of ApoE and C4b1 protein in 38 cases of pancreatic carcinoma tissues and adjacent normal pancreatic tissues, and RT-PCR was used to detect the expression of ApoE and C4b1 mRNA in 20 cases of pancreatic carcinoma tissues and adjancent normal pancreatic tissues. The relevance of ApoE and C4b1 expressions to the biological features of pancreatic carcinoma were analyzed. Results The positive rates of ApoE and C4b1 expressions are 86.8% (33/38) and 76.3% (29/38) in pancreatic carcinoma, respectively,which were significantly higher than those in normal pancreatic tissues [42.1% (16/38) and 26.3% ( 10/38 ),P ＜ 0.01]. The positive rates of ApoE and C4b1 expressions [78.3% ( 18/23 ) and 73.9% ( 17/23 )] in patients with metastasis were significantly higher than in those without metastasis [(33.3% (5/15) and 40.0%(6/15), P ＜ 0.05). Significantly higher expressions of ApoE and C4b1 mRNA were noted in pancreatic carcinoma(4.83 ± 0.65 and 7.94 ± 0. 95 ) than those in the normal pancreatic tissue ( 1.78 ± 0.74and 1.22 ±0.57, P ＜ 0.01 ), and patients with metastasis showed significantly higher expression of ApoE and C4b1 mRNA (5.05 ±0.71 and 8.24 ± 1.07) than those without metastasis (4.42 ±0.25 and 7.39 ±0.15,P ＜ 0.05). Conclusions ApoE and C4b1 were highly expressed in pancreatic cancer, and may be closely related with lymph node metastasis.
