中华小儿外科杂志
中華小兒外科雜誌
중화소인외과잡지
CHINESE JOURNAL OF PEDIATRIC SURGERY
2012年
5期
374-377
,共4页
张奇%汪健%黄顺根%李毅平%潘江
張奇%汪健%黃順根%李毅平%潘江
장기%왕건%황순근%리의평%반강
手术应激%婴幼鼠%吞噬受体%细菌杀灭能力
手術應激%嬰幼鼠%吞噬受體%細菌殺滅能力
수술응격%영유서%탄서수체%세균살멸능력
Surgical stress%Infant mice%Phagocytic receptors%Bactericidal ability
目的 吞噬作用是宿主固有免疫抵御外来入侵病原微生物的一个关键因素.手术应激可产生机体免疫抑制,增加感染的危险.本研究旨在探讨手术应激对成年鼠和婴幼鼠吞噬受体表达及其细菌杀灭能力的影响,分析吞噬体成熟及杀菌机制,进而为临床干预提供依据.方法 取婴幼(1～2周龄)和成年(8～10周龄)Balb/C小鼠各40只,分为对照组和手术应激组.腹腔灌洗法分别取术后24 h和对照组腹腔巨噬细胞,流式细胞仪测定巨噬细胞表面吞噬受体FcγR和CR3的表达；巨噬细胞与活菌共同孵育不同时间,计数细菌菌落生长数,计算胞内细菌杀灭数.结果 经流式细胞仪测定,对照组婴幼鼠巨噬细胞吞噬受体CR3和FcγR荧光强度分别为261.77±22.75和38.42±4.29,较对照组成年鼠(453.33±23.80、43.07±12.01)表达低(P＜0.05);手术应激后,婴幼鼠表达(49.87±3.04、9.57±0.76)明显下降(P＜0.05),而成年鼠表达(263.00±9.33、45.43±1.98)下降不明显.与细菌共培养30 min和60 min后,细菌吞噬率分别为43.75％±4.70％和61.29％±10.94％,较成年鼠(58.34％±8.87％和76.02％±3.64％)低(P＜0.05),手术应激后,婴幼鼠细菌吞噬率(38.06％±12.90％和56.15％±11.00％)亦有所下降.结论 婴幼鼠较成年鼠吞噬受体表达低,细菌杀灭能力弱.手术应激后,婴幼鼠吞噬受体的表达进一步降低,吞噬功能明显受损,细菌杀灭能力进一步下降,从而增加了术后感染的风险.
目的 吞噬作用是宿主固有免疫牴禦外來入侵病原微生物的一箇關鍵因素.手術應激可產生機體免疫抑製,增加感染的危險.本研究旨在探討手術應激對成年鼠和嬰幼鼠吞噬受體錶達及其細菌殺滅能力的影響,分析吞噬體成熟及殺菌機製,進而為臨床榦預提供依據.方法 取嬰幼(1～2週齡)和成年(8～10週齡)Balb/C小鼠各40隻,分為對照組和手術應激組.腹腔灌洗法分彆取術後24 h和對照組腹腔巨噬細胞,流式細胞儀測定巨噬細胞錶麵吞噬受體FcγR和CR3的錶達；巨噬細胞與活菌共同孵育不同時間,計數細菌菌落生長數,計算胞內細菌殺滅數.結果 經流式細胞儀測定,對照組嬰幼鼠巨噬細胞吞噬受體CR3和FcγR熒光彊度分彆為261.77±22.75和38.42±4.29,較對照組成年鼠(453.33±23.80、43.07±12.01)錶達低(P＜0.05);手術應激後,嬰幼鼠錶達(49.87±3.04、9.57±0.76)明顯下降(P＜0.05),而成年鼠錶達(263.00±9.33、45.43±1.98)下降不明顯.與細菌共培養30 min和60 min後,細菌吞噬率分彆為43.75％±4.70％和61.29％±10.94％,較成年鼠(58.34％±8.87％和76.02％±3.64％)低(P＜0.05),手術應激後,嬰幼鼠細菌吞噬率(38.06％±12.90％和56.15％±11.00％)亦有所下降.結論 嬰幼鼠較成年鼠吞噬受體錶達低,細菌殺滅能力弱.手術應激後,嬰幼鼠吞噬受體的錶達進一步降低,吞噬功能明顯受損,細菌殺滅能力進一步下降,從而增加瞭術後感染的風險.
목적 탄서작용시숙주고유면역저어외래입침병원미생물적일개관건인소.수술응격가산생궤체면역억제,증가감염적위험.본연구지재탐토수술응격대성년서화영유서탄서수체표체급기세균살멸능력적영향,분석탄서체성숙급살균궤제,진이위림상간예제공의거.방법 취영유(1～2주령)화성년(8～10주령)Balb/C소서각40지,분위대조조화수술응격조.복강관세법분별취술후24 h화대조조복강거서세포,류식세포의측정거서세포표면탄서수체FcγR화CR3적표체；거서세포여활균공동부육불동시간,계수세균균락생장수,계산포내세균살멸수.결과 경류식세포의측정,대조조영유서거서세포탄서수체CR3화FcγR형광강도분별위261.77±22.75화38.42±4.29,교대조조성년서(453.33±23.80、43.07±12.01)표체저(P＜0.05);수술응격후,영유서표체(49.87±3.04、9.57±0.76)명현하강(P＜0.05),이성년서표체(263.00±9.33、45.43±1.98)하강불명현.여세균공배양30 min화60 min후,세균탄서솔분별위43.75％±4.70％화61.29％±10.94％,교성년서(58.34％±8.87％화76.02％±3.64％)저(P＜0.05),수술응격후,영유서세균탄서솔(38.06％±12.90％화56.15％±11.00％)역유소하강.결론 영유서교성년서탄서수체표체저,세균살멸능력약.수술응격후,영유서탄서수체적표체진일보강저,탄서공능명현수손,세균살멸능력진일보하강,종이증가료술후감염적풍험.
Objective Phagocytosis is a key element of the host innate immune system to fight against microbial pathogens.Macrophages detect pathogens through several phagocytic receptors such as Fcγ receptors and complement receptors.The combination of receptors and pathogens activates various intracellular signal transduction pathways.Surgical stress will cause immune suppression and increases the risk of infections postoperatively.Our research was to investigate the effect of surgical stress on the expression of phagocytic receptors and the bactericidal activity in a mice model.Methods Infant (aged 1-2 weeks) and adults (aged 8 10 weeks) Balb/c mice were randomized to control and surgical stress group (n =20).Peritoneal lavage was collected from both groups 24hrs postoperatively,FACScan analysis was performed to detect the surface expression of CR3 and FcγR on macrophages.After co-culture of peritoneal macrophages and Escherichia coli for different time duration,the number of bacterial colony was counted,and the bactericidal ability of macrophages was calculated.Results The expression of phagocytic receptors CR3 and FcγR on the infant macrophage were lower than in the adult in the control group (P＜0.05).After surgical stress,the expressions of phagocytic receptors the infant macorphage were more downregulated than in the adult group (P＜0.05).The infant macrophage was weaker in the bactericidal activity after 30min and 60min co-incubation than in the adult.This was also reduced in infant macrophage after surgical stress.Conclusions The expression of phagocytic receptors on macrophages and the function of infant mice was lower and they are inefficient in killing bacteria.That may increase the risk of infections postoperatively.
