CAJ | 학술논문

目的对大鼠成骨细胞施加单轴向周期性牵张应力,观察单轴向周期性牵张应力对大鼠成骨细胞细胞骨架的影响.方法无菌环境下,组织分离法培养SD大鼠颅骨成骨细胞,体外培养,应用DioDynamic细胞应力加载系统分别对2组第3代大鼠成骨细胞加载0%和2%的单轴向周期性牵张应力,使用免疫荧光法检测细胞骨架蛋白表达,使用激光共聚焦显微镜观察细胞骨架的变化,随即抽取视野并测量各组细胞骨架蛋白表达的荧光强度,进行统计学分析.结果经统计学计算,施加单轴向周期性牵张应力实验组与空白对照组相比,实验组大鼠成骨细胞骨架蛋白的荧光表达减弱;应力纤维变细,明显沿受力方向分布.结论 DioDynamic细胞应力加载系统可以很好地模拟生物体内成骨细胞所受单向周期牵张应力;大鼠成骨细胞细胞骨架感知单轴向周期性牵张应力,参与细胞的力学信号转导和细胞形态重构.
목적 대대서성골세포시가단축향주기성견장응력,관찰단축향주기성견장응력대대서성골세포세포골가적영향.방법 무균배경하,조직분리법배양SD대서로골성골세포,체외배양,응용DioDynamic세포응력가재계통분별대2조제3대대서성골세포가재0%화2%적단축향주기성견장응력,사용면역형광법검측세포골가단백표체,사용격광공취초현미경관찰세포골가적변화,수즉추취시야병측량각조세포골가단백표체적형광강도,진행통계학분석.결과 경통계학계산,시가단축향주기성견장응력실험조여공백대조조상비,실험조대서성골세포골가단백적형광표체감약;응력섬유변세,명현연수력방향분포.결론 DioDynamic세포응력가재계통가이흔호지모의생물체내성골세포소수단향주기견장응력;대서성골세포세포골가감지단축향주기성견장응력,삼여세포적역학신호전도화세포형태중구.
Objective To investigate the effects of mechanical strain on osteoblast cytoskeleton of rat by DioDynamic Testing System. Methods Osteoblasts were retrieved from SD rats' skull in the sterilized environment. DioDynamic testing system was used to form 0% and 2% mechanical strain on two groups of the third generation rats' osteoblasts. Immunofluorescent was used to detect expression of F-actin in the two groups of osteoblasts. Leica Microsystem software was applied to evaluate the fluorescence of F-actin expressed among the two groups of samples. Results Significant decrease was observed on the expression of protein F-actin in the 2%mechanical strain experinental group compared to the control group (0% mechanical strain). Conclusion The DioDynamic testing system can successfully simulate the mechanical strain in vitro. Osteoblast cytoskeleton of rat participates in mechanical cell signal transduction and cell morphological reconstruction by detecting the mechanical strain.