中华麻醉学杂志
中華痳醉學雜誌
중화마취학잡지
CHINESE JOURNAL OF ANESTHESIOLOGY
2009年
4期
346-348
,共3页
吗啡%脊髓%长时程增强%电刺激%坐骨神经
嗎啡%脊髓%長時程增彊%電刺激%坐骨神經
마배%척수%장시정증강%전자격%좌골신경
Morphine%Spinal cord%Long-term potentiation%Electric stimulation%Sciatic nerve
目的 评价吗啡对电刺激坐骨神经诱发大鼠脊髓背角突触长时程增强(LTP)的影响.方法 雄性SD大鼠27只,日龄60～90 d,体重180～200 g,随机分为4组:对照组(C组,n=7)、吗啡组(M组,n=7)、纳洛酮组(N组,n=6),纳洛酮+吗啡组(MN组,n=7).麻醉下分离左侧坐骨神经,记录电极插入左侧T13～L1脊髓背角,刺激电极刺激左侧坐骨神经,给予15 V、0.5 ms、1/60 Hz单个方波电刺激30 min以诱发场电位,抽取生理盐水10 μl、吗啡10 μl(15 μg/μl)、纳洛酮10 μl(2.5 μg/μl)、纳洛酮(2.5 μg/μl)和吗啡(15 μg/μl)各5 μl的混合液,在脊髓上方3～5 mm,经2 min内缓慢滴注,给药后5 min时,给予4串高频高强度强直电刺激后,再给予15 V、0.5 ms、1/60 Hz单个方波电刺激210 min,记录强直刺激前30 min、强直刺激后即刻～30 min、35～60 min、65～120 min、125～210 min时段平均场电位幅值及潜伏期.结果 与C组比较,M组和MN组平均场电位幅值降低,潜伏期延长(P<0.05或0.01),N组上述指标差异无统计学意义(P>0.05).与M组比较,MN组平均场电位幅值升高,潜伏期缩短(P<0.05或0.01).与强直刺激前30 min比较,C组和N组在强直刺激后各时段平均场电位幅值升高,潜伏期缩短,M组在强直刺激后各时段平均场电位幅值降低,潜伏期延长,MN组在强直刺激后即刻～30 min和35～60 min时段平均场电位幅值升高,强直刺激后即刻～30 min时段潜伏期缩短,65～120 min和125～210 min时段平均场电位幅值降低,潜伏期延长(P<0.05或0.01).结论 吗啡可抑制电刺激坐骨神经诱发大鼠脊髓背角突触LTP,可能是其抑制中枢敏化的机制之一.
目的 評價嗎啡對電刺激坐骨神經誘髮大鼠脊髓揹角突觸長時程增彊(LTP)的影響.方法 雄性SD大鼠27隻,日齡60～90 d,體重180～200 g,隨機分為4組:對照組(C組,n=7)、嗎啡組(M組,n=7)、納洛酮組(N組,n=6),納洛酮+嗎啡組(MN組,n=7).痳醉下分離左側坐骨神經,記錄電極插入左側T13～L1脊髓揹角,刺激電極刺激左側坐骨神經,給予15 V、0.5 ms、1/60 Hz單箇方波電刺激30 min以誘髮場電位,抽取生理鹽水10 μl、嗎啡10 μl(15 μg/μl)、納洛酮10 μl(2.5 μg/μl)、納洛酮(2.5 μg/μl)和嗎啡(15 μg/μl)各5 μl的混閤液,在脊髓上方3～5 mm,經2 min內緩慢滴註,給藥後5 min時,給予4串高頻高彊度彊直電刺激後,再給予15 V、0.5 ms、1/60 Hz單箇方波電刺激210 min,記錄彊直刺激前30 min、彊直刺激後即刻～30 min、35～60 min、65～120 min、125～210 min時段平均場電位幅值及潛伏期.結果 與C組比較,M組和MN組平均場電位幅值降低,潛伏期延長(P<0.05或0.01),N組上述指標差異無統計學意義(P>0.05).與M組比較,MN組平均場電位幅值升高,潛伏期縮短(P<0.05或0.01).與彊直刺激前30 min比較,C組和N組在彊直刺激後各時段平均場電位幅值升高,潛伏期縮短,M組在彊直刺激後各時段平均場電位幅值降低,潛伏期延長,MN組在彊直刺激後即刻～30 min和35～60 min時段平均場電位幅值升高,彊直刺激後即刻～30 min時段潛伏期縮短,65～120 min和125～210 min時段平均場電位幅值降低,潛伏期延長(P<0.05或0.01).結論 嗎啡可抑製電刺激坐骨神經誘髮大鼠脊髓揹角突觸LTP,可能是其抑製中樞敏化的機製之一.
목적 평개마배대전자격좌골신경유발대서척수배각돌촉장시정증강(LTP)적영향.방법 웅성SD대서27지,일령60～90 d,체중180～200 g,수궤분위4조:대조조(C조,n=7)、마배조(M조,n=7)、납락동조(N조,n=6),납락동+마배조(MN조,n=7).마취하분리좌측좌골신경,기록전겁삽입좌측T13～L1척수배각,자격전겁자격좌측좌골신경,급여15 V、0.5 ms、1/60 Hz단개방파전자격30 min이유발장전위,추취생리염수10 μl、마배10 μl(15 μg/μl)、납락동10 μl(2.5 μg/μl)、납락동(2.5 μg/μl)화마배(15 μg/μl)각5 μl적혼합액,재척수상방3～5 mm,경2 min내완만적주,급약후5 min시,급여4천고빈고강도강직전자격후,재급여15 V、0.5 ms、1/60 Hz단개방파전자격210 min,기록강직자격전30 min、강직자격후즉각～30 min、35～60 min、65～120 min、125～210 min시단평균장전위폭치급잠복기.결과 여C조비교,M조화MN조평균장전위폭치강저,잠복기연장(P<0.05혹0.01),N조상술지표차이무통계학의의(P>0.05).여M조비교,MN조평균장전위폭치승고,잠복기축단(P<0.05혹0.01).여강직자격전30 min비교,C조화N조재강직자격후각시단평균장전위폭치승고,잠복기축단,M조재강직자격후각시단평균장전위폭치강저,잠복기연장,MN조재강직자격후즉각～30 min화35～60 min시단평균장전위폭치승고,강직자격후즉각～30 min시단잠복기축단,65～120 min화125～210 min시단평균장전위폭치강저,잠복기연장(P<0.05혹0.01).결론 마배가억제전자격좌골신경유발대서척수배각돌촉LTP,가능시기억제중추민화적궤제지일.
Objective To evaluate the effect of morphine on synaptic long-term potentiation (LTP) in the spinal dorsal horn evoked by electric stimulation of sciatic nerve in rats. Methods Twenty-seven healthy male SD rats aged 60-90 d weighing 180-200 g were randomly divided into 4 groups: group Ⅰ control (group C, n=7), group Ⅱ morphine (group M, n=7), group Ⅲ naloxone (group N, n=6), and group Ⅳ morphine + naloxone (group MN, n=7). The animals were anesthetized with intraperitoneal 10% urethane 1 g/kg, intubated and then mechanically ventilated. The bipolar insulated stainless steel recording electrode (impedance 0.5-1 MΩ, diameter 0.1 mm) was inserted into the left side of the spinal dorsal horn at T13-L1 to stimulate the left side of the sciatic nerve. Single square pulses (15 V, 0.5 ms, 1/60 Hz for 30 min) was applied to evoke spinal field potentials. Normal saline 10 μl, morphine 10 μl (15 μg/μl), naloxone 10 μl (2.5 μg/μl), and the mixture 10 μl of naloxone 5 μl (2.5 μg/μl) and morphine 5 μl (15 μg/μl) was gradually instilled over 2 rain in the 4 groups respectively. Five minutes later, high-frequency and intensity tetanic stimulation (30-40 V, 0.5 ms, 100 Hz, given in 4 trains of 1-s duration at 10-s intervals) was used to induce LTP. Then single square stimuli (15 V, 5 ms, 1/60 Hz) were applied to the sciatic nerve for 210 min. The amplitude and latency period of the field potential were recorded 30 min before tetanic stimulation, and 0-30, 35-60, 65-120 and 125-210 min after titanic stimulation. Results Compared with group C, the amplitude of the field potential was significantly decreased and the latency period prolonged in group M and MN, but there was no significant difference in the above indices between group N and C. Compared with group M, the amplitude of the field potential was significantly increased and the latency period shortened in group MN. Compared with those 30 min before the tetanic stimulation, the amplitude of the field potential was significantly increased and latency period shorted at the time points after the tetanie stimulation in group C and N, the amplitude of the field potential was significantly decreased and latency period prolonged at the time points after the tetanie stimulation in group M, and the amplitude of the field potential was significantly increased 0-30 and 35-60 min after the tetanic stimulation and latency period shortened 0-30 min after the tetanie stimulation, the amplitude of the field potential was significantly decreased and latency period prolonged 65-120 and 125-210 min after the tetanic stimulation in group MN. Conclusion Morphine can inhibit synaptic LTP in the spinal dorsal horn evoked by electric stimulation of sciatic nerve in rats, and it may be one of the mechanisms of the central sensitization inhibition.
