CAJ | 학술논문

目的:p73基因是与p53相类似的基因,在人类神经母细胞瘤中被假定作为肿瘤抑制基因.为了证实p73在非小细胞肺癌中是否也是肿瘤抑制基因,我们应用Sty I内切酶多态分析法研究了6株非小细胞肺癌细胞的等位基因表达模式.方法:利用RT-PCR检测p73基因在这6株肺癌细胞中转录水平的表达,同时用免疫组织化学方法检测5株非小细胞肺癌细胞所诱发的裸鼠种植瘤中P73蛋白的表达.结果:p73基因在这6株非小细胞肺癌中均为纯合性等位基因表达,而GC/GC基因型为主要类型.p73基因在转录水平和蛋白水平完全丧失表达.结论:根据实验结果可推测,p73在这6株非小细胞肺癌细胞中仍扮演了肿瘤抑制基因的角色.
목적:p73기인시여p53상유사적기인,재인류신경모세포류중피가정작위종류억제기인.위료증실p73재비소세포폐암중시부야시종류억제기인,아문응용Sty I내절매다태분석법연구료6주비소세포폐암세포적등위기인표체모식.방법:이용RT-PCR검측p73기인재저6주폐암세포중전록수평적표체,동시용면역조직화학방법검측5주비소세포폐암세포소유발적라서충식류중P73단백적표체.결과:p73기인재저6주비소세포폐암중균위순합성등위기인표체,이GC/GC기인형위주요류형.p73기인재전록수평화단백수평완전상실표체.결론:근거실험결과가추측,p73재저6주비소세포폐암세포중잉분연료종류억제기인적각색.
AIM,To verify whether p73;a homologue of p53,which supposed]y acts as a tumor suppressor gene in neuroblastoma,might also be a tumor suppressor in non-small cell lung cancer.METHODS:The allelic expres-sion of p73 in the six non-small cell lung cancer cell lines was studied by Sty I polymorphism analysis.The P73 gene ex.pressions in these six cell lines were examined by reverse transcription-PCR,the expressions of P73 protein in the five cell lines inducing tumor8 were also determined by immunohistochemistry.RESULTS:Homozygous allelic expression was dem.onstrated in all six cell lines and the GC/GC genotype Was the predominant type.Complete loss of the p73 expression both at mRNA and the protein level was revealed.CONCLUSION:Taken together,our data suggest that p73 might play a role as a tumor suppressor gene in human non-small cell lung cancer cell lines.