CAJ | 학술논문

采用DGGE指纹图谱技术对新疆乌苏的泥火山区的土壤真菌群落18SrDNA进行电泳分离，通过Quantity one软件对图谱进行聚类分析，并对主要条带进行回收测序。DGGE图谱聚类分析显示，五月份表层土真菌的种类和数量丰富。群落结构在相同季节垂直分布上相似性较高，聚集到一个分支上；而不同季节土样真菌组成差异较大。特异条带的回收测序结果表明，泥火山真菌优势菌群为链格孢属真菌和多枝横梗霉属。新疆泥火山土壤真菌的多样性较高但数量偏少，受气候环境因素的影响较大，优势菌群受此影响较小。
채용DGGE지문도보기술대신강오소적니화산구적토양진균군락18SrDNA진행전영분리，통과Quantity one연건대도보진행취류분석，병대주요조대진행회수측서。DGGE도보취류분석현시，오월빈표층토진균적충류화수량봉부。군락결구재상동계절수직분포상상사성교고，취집도일개분지상；이불동계절토양진균조성차이교대。특이조대적회수측서결과표명，니화산진균우세균군위련격포속진균화다지횡경매속。신강니화산토양진균적다양성교고단수량편소，수기후배경인소적영향교대，우세균군수차영향교소。
Reveal the bacterial community structure and dynamics in mud volcano soil in Xinjiang. 18S-rDNA-ragment DGGE (denaturing gradient gel electrophoresis) and clone sequence BLAST technologies were applied, quantity one software was used to map the dendrogram. The cluster analysis of DGGE profiles showed that the number of species of fungi were the most abundant in the surface soil of May and community structure during the same season of the vertical distribution of high similarity, clustered into a branch; and fungi in soil samples composed of different seasons were quite different. The recovery of specific bands sequencing result showed that mud volcano fungi as Alternaria dominant fungi were Alternaria tenuissima. sp and Lichtheimia ramosa. sp. The fungi polymorphism in mud volcano soil in Xinjiang was significant, but the quantity was few, and this diversity was vulnerable to ecological, climatic and other factors. But the dominant microorganisms affected less.