CAJ | 학술논문

[目的]研究硅酸盐细菌的解钾作用,为利用该菌生产生物钾肥提供科学依据.[方法]从不同地区土样筛选硅酸盐细菌,选出高效菌株DMS3,并进行生理生化鉴定和16S rDNA的PCR分析,对DMS3菌株进行发酵,用发酵液浇灌绿豆,观察其对绿豆各生长指标的影响.[结果]DMS3菌株16S rDNA大约1 500 bp,该菌有较高的解钾能力,发酵液中K~+浓度达到1 020.646 μg/L,用该菌液浇灌绿豆可明显提高株高、根长、鲜重、干重等指标.[结论]硅酸盐细菌在液体培养过程中能够产生某些促进植物生长的物质,同时能提高绿豆的抗旱能力,为该菌肥工业化开发应用奠定了基础.
[목적]연구규산염세균적해갑작용,위이용해균생산생물갑비제공과학의거.[방법]종불동지구토양사선규산염세균,선출고효균주DMS3,병진행생리생화감정화16S rDNA적PCR분석,대DMS3균주진행발효,용발효액요관록두,관찰기대록두각생장지표적영향.[결과]DMS3균주16S rDNA대약1 500 bp,해균유교고적해갑능력,발효액중K~+농도체도1 020.646 μg/L,용해균액요관록두가명현제고주고、근장、선중、간중등지표.[결론]규산염세균재액체배양과정중능구산생모사촉진식물생장적물질,동시능제고록두적항한능력,위해균비공업화개발응용전정료기출.
[Objective] The research aimed to study the K-releasing effect of silicate bacteria provide scientific basis for producing bio-potassium fertilizers by using the bacteria. [Method] Silicate bacteria were selected from different regions. DMS3 was higher efficient strain and its physiological and biochemical characterization was identified. 16S rDNA gene of the bacteria was obtained by PCR amplification. Mung bean fed with fermentation of DMS3 was used to observe the impact on the growth indicators. [Result] 16S rDNA gene of the bacteria was about 1 500 bp. DMS3 fermentation had a high potassium concentration, reached K ion concentration 1 020.646 μg/L. The fermentation could significantly increase the plant height, root length, fresh weight, dry weight of mung bean. [Conclusion] Silicate bacteria in liquid culture can produce some substances to promote the growth of mung bean, at the same time to increase the capacity of drought. The research laid the foundation for the industrialization development and application of fertilizer.