CAJ | 학술논문

目的:建立甲基苯丙胺中毒致死的动物模型,探讨其在致死大白兔体内的分布规律.方法:用6只日本♂大白兔经胃管按照150 mg·kg~(-1)(3 LD_(50))给予盐酸甲基苯丙胺建立动物模型,大白兔死后迅速解剖并提取心、肝、脾、肺、肾、脑、肌肉、睾丸、胃、心血、周围血、胆汁、尿液和玻璃体液,用正己烷∶乙醇(20∶1)作为提取溶剂,利用UPLC- MS/MS法检测其中甲基苯丙胺含量,使用SPSS 15.0统计软件进行方差分析,采用均数两两比较的SNK法.检验水准为α=0.05.结果:实验大白兔胃内甲基苯丙胺含量最高,其次是脾、肺、肾、睾丸和尿液,而心血、周围血和玻璃体液中含量较低.结论:本实验的结果与相关案例报道基本吻合,这提示胃、脾、肾和尿液是甲基苯丙胺中毒案件鉴定的理想检材,中毒死后的甲基苯丙胺分布规律可为相关案件的鉴定提供一定的依据.
목적:건립갑기분병알중독치사적동물모형,탐토기재치사대백토체내적분포규률.방법:용6지일본♂대백토경위관안조150 mg·kg~(-1)(3 LD_(50))급여염산갑기분병알건립동물모형,대백토사후신속해부병제취심、간、비、폐、신、뇌、기육、고환、위、심혈、주위혈、담즙、뇨액화파리체액,용정기완∶을순(20∶1)작위제취용제,이용UPLC- MS/MS법검측기중갑기분병알함량,사용SPSS 15.0통계연건진행방차분석,채용균수량량비교적SNK법.검험수준위α=0.05.결과:실험대백토위내갑기분병알함량최고,기차시비、폐、신、고환화뇨액,이심혈、주위혈화파리체액중함량교저.결론:본실험적결과여상관안례보도기본문합,저제시위、비、신화뇨액시갑기분병알중독안건감정적이상검재,중독사후적갑기분병알분포규률가위상관안건적감정제공일정적의거.
Objective:To establish the models of methamphetamine poisoned death models, and investigate the distribution of methamphetamine in poisoned death rabbits. Methods:Six male rabbits were given a dose of 150 mg·kg~(-1) weight of methamphetamine by gastric tube at an even speed in three minutes. The heart, lung, liver, spleen, kidney, cerebrum, muscle, spermary, gastric, heart blood, peripheral blood, bile, urine and vitreous were collected and analyzed immediately after the death. These samples were extracted by n-hexane∶ ethanol (v/v, 20∶1). Analysis was performed with a UPLC-MS/MS. The qualitative analysis was based on retention time in the full-scan chromatogram of MS1 coupled with the ion fragmentation spectrum of MS_2 in the mass spectrometer. The quantitative analysis was on a working curve method. Results:The concentration detected in gastric was the highest and there was a statistic difference (P<0.05). The concentrations detected in spleen, lung, kidney, spermary and urine were also higher. Conclusion:The results were in accord with correlative case reports. Gastric, spleen, kidney and urine may be the ideal samples for forensic identification and the postmortem distribution of methamphetamine may provide some evidence for the investigation of suspected poisoning case.