CAJ | 학술논문

目的探讨过氧化氢诱导喉癌细胞Hep-2凋亡过程中PDCD4基因表达的变化.方法以体外培养的喉癌细胞Hep-2为实验材料,不同浓度的过氧化氢作用于Hep-2细胞,噻唑蓝(MTT)比色法测定细胞生存率,采用吖啶橙染色、Ho33342/PI荧光双染进行形态学观察, RT-PCR及Western blot 检测PDCD4 mRNA水平及蛋白表达的变化,评价在过氧化氢诱导喉癌细胞Hep-2凋亡过程中PDCD4基因的作用.结果过氧化氢 (200 μmol/L) 作用Hep-2细胞24h,能够显著抑制细胞增殖,并诱导细胞凋亡,同时引起pdcd4 mRNA水平显著上调,PDCD4蛋白表达显著增加.结论本研究首次报道PDCD4基因可能在氧化胁迫诱导喉癌细胞凋亡中起关键作用.
목적 탐토과양화경유도후암세포Hep-2조망과정중PDCD4기인표체적변화.방법 이체외배양적후암세포Hep-2위실험재료,불동농도적과양화경작용우Hep-2세포,새서람(MTT)비색법측정세포생존솔,채용아정등염색、Ho33342/PI형광쌍염진행형태학관찰, RT-PCR급Western blot 검측PDCD4 mRNA수평급단백표체적변화,평개재과양화경유도후암세포Hep-2조망과정중PDCD4기인적작용.결과 과양화경 (200 μmol/L) 작용Hep-2세포24h,능구현저억제세포증식,병유도세포조망,동시인기pdcd4 mRNA수평현저상조,PDCD4단백표체현저증가.결론 본연구수차보도PDCD4기인가능재양화협박유도후암세포조망중기관건작용.
Objective To investigate the change of PDCD4 gene expression in laryngeal carcinoma Hep-2 cells in response to the oxidative stress by hydrogen peroxide (H2O2).Methods Hep-2 cells were cultured in vitro and subjected to different concentrations of H2O2. Cell viability was measured by MTT assay. The morphological changes were assessed by acridine orange staining and Ho33342/PI double staining to determine whether the cells experienced apoptosis under the oxidative stress. In particular, the changes in the expression of PDCD4 gene at both mRNA and protein levels were examined by RT-PCR and Western-blot simultaneously.Results Direct exposure of Hep-2 cells to H2O2 was able to block cell proliferation, which was further revealed via the apoptotic pathway. Under such an oxidative condition, the expression of PDCD4 gene was apparently increased at both mRNA and protein levels.Conclusion The present study demonstrates, for the first time, that PDCD4 gene may play a pivotal role in the H2O2-induced apoptosis of Hep-2 cells.