CAJ | 학술논문

目的:通过实验评价聚乳酸乙醇酸/RNA Ⅲ抑制肽缓释微球的组织相容性.方法:采用Fmoc法由C端至N端先合成粗品肽;采用反相液相色谱法对RNA Ⅲ抑制肽粗品进行纯化分析,按紫外吸收峰收集组分,冷冻干燥,得到RNA Ⅲ抑制肽纯品.再采用液相复乳法制备直径50-70 μm的聚乳酸乙醇酸/RNA Ⅲ抑制肽微球.以急性全身毒性试验、MTT细胞毒性试验、肌肉内植入试验、过敏试验、热原试验对其组织相容性进行初步评价.结果:①急性全身毒性试验结果显示3组动物无中毒反应,无动物死亡,体质量无明显变化.②MTT细胞毒性试验结果显示两种浸提液的平均细胞增殖率均大于85%,细胞毒等级1级,不具有细胞毒性.③肌肉内植入试验结果显示RNA Ⅲ抑制肽粉末和聚乳酸乙醇酸/RNA Ⅲ抑制肽微球植入4周后,组织未见明显充血、变性或坏死.RNA Ⅲ抑制肽粉末完全降解,微球部分降解,主要细胞成分为纤维母细胞,未见中性粒细胞及多核巨细胞等炎症细胞浸润.④过敏试验结果显示3组动物的平均原发刺激指数分别为0.38、0.33和0.31,3组间无显著差别.⑤热原试验结果显示每种材料测试的3只新西兰大白兔中,体温升高均在0.5℃以下,并且体温升高总度数在1.3℃以下,符合热原实验的评价标准.结论:聚乳酸乙醇酸/RNA Ⅲ抑制肽微球具有良好的组织相容性.
목적:통과실험평개취유산을순산/RNA Ⅲ억제태완석미구적조직상용성.방법:채용Fmoc법유C단지N단선합성조품태;채용반상액상색보법대RNA Ⅲ억제태조품진행순화분석,안자외흡수봉수집조분,냉동간조,득도RNA Ⅲ억제태순품.재채용액상복유법제비직경50-70 μm적취유산을순산/RNA Ⅲ억제태미구.이급성전신독성시험、MTT세포독성시험、기육내식입시험、과민시험、열원시험대기조직상용성진행초보평개.결과:①급성전신독성시험결과현시3조동물무중독반응,무동물사망,체질량무명현변화.②MTT세포독성시험결과현시량충침제액적평균세포증식솔균대우85%,세포독등급1급,불구유세포독성.③기육내식입시험결과현시RNA Ⅲ억제태분말화취유산을순산/RNA Ⅲ억제태미구식입4주후,조직미견명현충혈、변성혹배사.RNA Ⅲ억제태분말완전강해,미구부분강해,주요세포성분위섬유모세포,미견중성립세포급다핵거세포등염증세포침윤.④과민시험결과현시3조동물적평균원발자격지수분별위0.38、0.33화0.31,3조간무현저차별.⑤열원시험결과현시매충재료측시적3지신서란대백토중,체온승고균재0.5℃이하,병차체온승고총도수재1.3℃이하,부합열원실험적평개표준.결론:취유산을순산/RNA Ⅲ억제태미구구유량호적조직상용성.
OBJECTIVE: To evaluate the histocompatibility of poly (lactic-co-glycolic acid)/RNA Ⅲ inhibiting peptide (PLGNRIP) sustained release microspheres.METHODS: The crude peptide comprising N to C-terminals was synthesized using Fmoc method. The crude synthetic RNAⅢ peptide was purified by reverse phase high performance liquid chromatography, followed by component harvesting according to ultraviolet absorption peak, and freeze-drying. PLGNRIP sustained release microspheres with a diameter of 50-70 pm were prepared using liquid-phase multiple emulsion method. The histocompatibility of PLGNRIP sustained release microscopes were preliminarily evaluated through the use of acute general toxicity test, MTT cytotoxicity test, intramuscular implantation test, sensitivity test, and pyrogen test.RESULTS: Acute general toxicity test results showed that all included animals survived and presented with no toxicosis reaction and obviously changed body mass. MTT cytotoxicity test results revealed that the average relative growth rate of cells from two eluents was over 85%, with cytotoxicity grade 1, which indicated no cytotoxicity. Intramuscular implantation tests showed that at 4 weeks after implantation of RiP powder or PLGNRIP microscopes, no obviously congested, degenerated, or necrotic tissue was observed. All RIP powder and a part PLGNRIP microscopes were degraded. Fibroblasts accounted for a large proportion in all cells. NO inflammatory cell infiltration, involving neutrophits and multinucleated giant celts, was observed. Sensitivity test rasults displayed that the average primary irritation index was 0.38, 0.33, arid 0.31 in the eluent stock solution, 2% dinitoflruorobenzene, and physiological saline-administerd groups, respectively. Pyrogen test results showed that fervescence of each rabbit in the experiment was under 0.5 ℃ and the sum of fervescence was under 1.3 ℃ .This is in coincidence with evaluation criteria of pyrogen test.CONCLUSION: PLGNRIP sustained release microspheras exhibit good histocompatibility.