CAJ | 학술논문

目的通过化肝解毒汤对慢性乙型肝炎模型大鼠血清白介素2(IL-2)、白介素4(IL-4)、干扰素-γ(IFN-γ)指标含量的影响,探讨化肝解毒汤治疗慢性乙型肝炎的作用机理.方法按照3 ml/kg体重,皮下注射40%四氯化碳(CCl4)橄榄油溶液,制成慢性肝损伤动物模型.将60只模型大鼠随机分为正常组、模型组、化肝解毒汤大组、化肝解毒汤小剂量组、乙肝清热解毒片组,各12只,检测各组血清IL-2、IL-4、IFN-γ含量,并进行比较.结果与正常组比较,模型组大鼠血清IL-2、IFN-γ指标降低(t值分别为2.401、2.337、P值分别为0.0349、0.0378),IL-4组含量升高(t=2.896、P=0.0086);与模型组比较,化肝解毒汤大剂量组、乙肝清热解毒片组IL-2、IFN-γ含量升高(前者t值分别为2.417、2.344,P值分别为0.0341、0.0372;后者t值分别为3.114、2.988,P值分别为0.0052、0.0068),化肝解毒汤小剂量组IFN-γ量升高(t=3.049,P=0.0062);用中药三组的IL-4含量均降低(t值分别为3.102、3.017、2.979,P值分别为0.0061、0.0065、0.0069).结论化肝解毒汤大剂量组可降低IL-4、升高IL-2、IFN-γ含量,调节th1/th2平衡.
목적 통과화간해독탕대만성을형간염모형대서혈청백개소2(IL-2)、백개소4(IL-4)、간우소-γ(IFN-γ)지표함량적영향,탐토화간해독탕치료만성을형간염적작용궤리.방법 안조3 ml/kg체중,피하주사40%사록화탄(CCl4)감람유용액,제성만성간손상동물모형.장60지모형대서수궤분위정상조、모형조、화간해독탕대조、화간해독탕소제량조、을간청열해독편조,각12지,검측각조혈청IL-2、IL-4、IFN-γ함량,병진행비교.결과 여정상조비교,모형조대서혈청IL-2、IFN-γ지표강저(t치분별위2.401、2.337、P치분별위0.0349、0.0378),IL-4조함량승고(t=2.896、P=0.0086);여모형조비교,화간해독탕대제량조、을간청열해독편조IL-2、IFN-γ함량승고(전자t치분별위2.417、2.344,P치분별위0.0341、0.0372;후자t치분별위3.114、2.988,P치분별위0.0052、0.0068),화간해독탕소제량조IFN-γ량승고(t=3.049,P=0.0062);용중약삼조적IL-4함량균강저(t치분별위3.102、3.017、2.979,P치분별위0.0061、0.0065、0.0069).결론 화간해독탕대제량조가강저IL-4、승고IL-2、IFN-γ함량,조절th1/th2평형.
Objective To explore the mechanism of Huagan Jiedu decoction in treatment of chronic hepatitis B by testing the influence of the decoction on Serum IL-2, IL-4 and IFN-γ of rat models of chronic hepatic injury. Methods The model of Chronic hepatic injury was established by subcutaneously injecting 40% CCl4-olive oil solution in rats according to the ratio of 3ml per kg. of body weight. 60 healthy rats were divided randomly into a normal group, a model group, a low dose of Huagan Jiedu decoction group, a large dose of Huagan Jiedu decoction group, and a control group (Qingre Jiedu tablet group), 15 cases in each group. The Serum IL-2, IL-4 and IFN-γ were detected in each group separately. Results Compared with the normal group, the Serum IL-2 and IFN-γ were decreased (t=2.401, 2.337, P=0.0349, 0.0378) and the Serum IL-4 was increased (t=2.896, P=0.00861 ) in the model group; Compared with the model group, the Serum IL-2 and IFN-γ were increased (t=2.417, 2.344, P=0.0341, 0.0372, P＜0.05; t=3.114, 2.988, P=0.0052, 0.0068) in the large Huagan Jiedu decoction group and the control group, while the Serum IFN-γ was increased (t=3.049, P=0.0062) in the low dose of Huagan Jiedu decoction group and the Serum IL-4 was decreased (t=3.102, 3.017, 2.979, P=0.0061, 0.0065, 0.0069)in both Huagan Jiedu decoction groups and the control group. Conclusion Large dose of Huagan Jiedu decoction has sound therapeutic effects on rats with chronic hepatic injury by decreaseing the content of the Serum IL-4 and increasing IL-2 and IFN-γ, and adjusting th1/th2 balance.