CAJ | 학술논문

目的观察创伤性休克大鼠肝组织HSP70 mRNA表达变化及甘氨酸的干预影响.方法检测甘氨酸治疗组、体克组和对照组肝组织HSP70 mRNA表达;观察肝组织病理改变并测定血清ALT和AST水平.结果休克组和治疗组肝组织HSP70mRNA表达分别于复苏后6、12 h达高峰.血清ALT、AST随时间延长逐渐增高;光镜下病理改变也随时间延长而损害加重.与休克组比较,治疗组肝组织HSP70 mRNA表达明显增强、血清ALT和AST明显降低且肝组织光镜下病理损害明显改善(P均<0.05).结论甘氨酸可增强肝组织HSP70表达,减轻创伤性休克后继发的肝脏损害.
목적 관찰창상성휴극대서간조직HSP70 mRNA표체변화급감안산적간예영향.방법 검측감안산치료조、체극조화대조조간조직HSP70 mRNA표체;관찰간조직병리개변병측정혈청ALT화AST수평.결과 휴극조화치료조간조직HSP70mRNA표체분별우복소후6、12 h체고봉.혈청ALT、AST수시간연장축점증고;광경하병리개변야수시간연장이손해가중.여휴극조비교,치료조간조직HSP70 mRNA표체명현증강、혈청ALT화AST명현강저차간조직광경하병리손해명현개선(P균<0.05).결론 감안산가증강간조직HSP70표체,감경창상성휴극후계발적간장손해.
Objective To investigate the dynamic changes of HSP70 mRNA expression in the liver tissue of rats with traumatic shock and the treatment effects of glycine.Methods The expression of HSP70 mRNA in the liver tissue of treatment group,shock group and control group was detected by ELISA.Pathological changes were observed,and serum ALT and AST were measured.Results The expression of HSP70 mRNA in the liver tissue of rats in the shock group and the treatment group reached peak at the 6th and 12th hour after resuscitation respectively.Serum ALT and AST increased and pathological damage aggravated with time prolonging.Compared with control group,the expression of HSPT0 mRNA in treatment group increased significantly,serum ALT and AST decreased significantly and pathologi- cal damage was significantly relieved(all P<0.05).Conclusion Glycine can increase the expression of HSPT0 mRNA and relieve the secondary damage of liver after traumatic shock.