CAJ | 학술논문

目的通过制备大鼠皮质-纹状体-黑质脑片,在可视条件下观察纹状体中等多棘神经元(MSN)的电活动,探讨其电生理特性.方法选用出牛7～10 d的健康SD大鼠,制备皮质-纹状体-黑质旁矢状位脑片,通过红外微分干涉相差(IR-DIC)显微镜直视下定位纹状体MSN,并采用膜片钳放大器全细胞记录,电流钳模式下记录MSN的自发性电活动,采用步阶电流注入,观察膜电位变化.结果成功记录的92个MSN表现为三种状态:14个细胞为持续的极化状态,无动作电位发放;61个细胞表现为持续的极化状态间隔短阵的去极化至阈电位水平伴发动作电位;17个细胞为持续的极化状态间隔突然出现的去极化状态.三种表现形式细胞的静息电位、阈电位均数差异无统计学意义(P>0.05).注入电流时,膜电位变化表现为一定程度的延迟,电位变化随注入电流增强有减少趋势.结论旁矢状位脑片中的纹状体MSN保留了在体的电生理特性,为深入研究黑质-纹状体通路电信号的发生和传递在帕金森病发病机制中的作用奠定了基础.
목적 통과제비대서피질-문상체-흑질뇌편,재가시조건하관찰문상체중등다극신경원(MSN)적전활동,탐토기전생리특성.방법 선용출우7～10 d적건강SD대서,제비피질-문상체-흑질방시상위뇌편,통과홍외미분간섭상차(IR-DIC)현미경직시하정위문상체MSN,병채용막편겸방대기전세포기록,전류겸모식하기록MSN적자발성전활동,채용보계전류주입,관찰막전위변화.결과 성공기록적92개MSN표현위삼충상태:14개세포위지속적겁화상태,무동작전위발방;61개세포표현위지속적겁화상태간격단진적거겁화지역전위수평반발동작전위;17개세포위지속적겁화상태간격돌연출현적거겁화상태.삼충표현형식세포적정식전위、역전위균수차이무통계학의의(P>0.05).주입전류시,막전위변화표현위일정정도적연지,전위변화수주입전류증강유감소추세.결론 방시상위뇌편중적문상체MSN보류료재체적전생리특성,위심입연구흑질-문상체통로전신호적발생화전체재파금삼병발병궤제중적작용전정료기출.
Objective To establish the neocortex-striatum-substantia nigra brain slices of rats and observe the medium spiny neurons of striatum under a visible condition so as to explore their electrophysiological characteristics. Methods The brain slices containing the neocortex-striatum-substantia nigra were prepared from SD rats of postnatal 7 - 10 days. With infrared differential interference contrast (IR-DIC) microscope and patch clamp amplifier whole-cell recording technique, the medium spiny neurons were located in striatum and their spontaneous electrical activity was recorded in the current clamp mode. By infusing the step current, we observed the variation of membrane potentials. Results There were three types of conditions in the 92 medium spiny neurons successfully recorded. Among them, 14 were in persistent down state without action potential firing; 61 displayed persistnet down state accompanied with interval temporal depolarizing to the threshold potential with action potential firing; and the remaining 17 showed persistent down state with the interval emergent up state. There was no statistical significance in the difference of mean resting and threshold potentials in three neuronal types ( P > 0. 05 ). When the neurons