CAJ | 학술논문

[目的]研究妊娠初期有机磷农药暴露对母体生殖功能,对子代细胞遗传学及生长发育的影响.[方法]以有机磷农药敌百虫为受试物,以着床期胚胎为研究对象,以反映染色体异常的微核形成和反映胚胎生长发育状况的构成细胞数为指标.孕鼠腹腔注射100或200 mg/kg剂量的敌百虫溶液,3d后,在体视显微镜下从其子宫中取出胚胎,进行其形态学分析,微核试验及胚胎构成细胞数的分析.[结果]敌百虫暴露组的胚胎微核发生率明显高于对照组,分别为200 mg/kg暴露组为16.9％,100 mg/kg暴露组16.1％,对照组为5.3％,并有明显的剂量-反应关系.此外,母鼠暴露于敌百虫,可造成子代生长发育迟缓,表现为胚胎构成细胞数明显下降(200mg/kg组44.1,对照组54.7).[结论]妊娠初期有机磷农药暴露对着床前期胚胎的细胞遗传学及生长发育有明显影响.着床前期小鼠胚胎的微核试验是评价母体早期暴露有机磷农药导致子代细胞遗传学改变的简便、灵敏的指标,本结果为进一步对着床后胚胎的研究,奠定了基础.
[목적]연구임신초기유궤린농약폭로대모체생식공능,대자대세포유전학급생장발육적영향.[방법]이유궤린농약활백충위수시물,이착상기배태위연구대상,이반영염색체이상적미핵형성화반영배태생장발육상황적구성세포수위지표.잉서복강주사100혹200 mg/kg제량적활백충용액,3d후,재체시현미경하종기자궁중취출배태,진행기형태학분석,미핵시험급배태구성세포수적분석.[결과]활백충폭로조적배태미핵발생솔명현고우대조조,분별위200 mg/kg폭로조위16.9％,100 mg/kg폭로조16.1％,대조조위5.3％,병유명현적제량-반응관계.차외,모서폭로우활백충,가조성자대생장발육지완,표현위배태구성세포수명현하강(200mg/kg조44.1,대조조54.7).[결론]임신초기유궤린농약폭로대착상전기배태적세포유전학급생장발육유명현영향.착상전기소서배태적미핵시험시평개모체조기폭로유궤린농약도치자대세포유전학개변적간편、령민적지표,본결과위진일보대착상후배태적연구,전정료기출.
[ Objective] Trichlorfon, an organophosphate pesticide, was evaluated for its ability to induce cytogenetic damage in preimplantation from dams treated with trichlorfon. [ Methods] Pregnant female mice were intraperitoneally(i. p. ) administered a single dose of trichlorfon ( 100 mg/kg or 200 mg/kg) during the early preimplantation period. On day 3 of gestation,blastocysts were collected and evaluated for gross morphology, micronuclei(MN) frequency and cell number. [ Results] MN was found in 16.9% of all preimplatation embryos in the 200mg/kg group, 16.1% in the 100 mg/kg group versus 5.3 % in control group, P ＜ 0.01. A significant increase in MN frequency indicating cytogenetic damage was observed in the treatment groups in comparison to control group. The MN frequency revealed a clear dose-dependent increase. There was also a significant decrease in the embryo cell number in the 80mg/kg treated group (44.1,versus 54.7 in control, P ＜ 0.05). A simultaneous decrease in the cell number and increase in MN frequency may reflect an embryonic developmental disadvantage resulting from maternal treatment with trichlorfon. [Conclusion] The findings of this study demonstrate that the cytogenetic toxicity of trichlorfon in the preimplantation embryos after maternal exposure to insecticide during early preimplantation period, MN test in preimplantation embryos is simple and effective.