CAJ | 학술논문

以2个高淀粉和2个低淀粉玉米自交系为材料,分析了玉米籽粒淀粉的动态积累规律,同时对高低淀粉玉米籽粒灌浆过程中淀粉生物合成关键酶活性的动态变化及其与淀粉积累动态的相关性进行讨论分析.研究结果表明:灌浆过程中4个自交系淀粉含量变化趋势均呈sigmoid型曲线.灌浆过程中ADPG-PPase(腺苷二磷酸葡萄糖焦磷酸化酶)、SSS(可溶性淀粉合成酶)、GBSS(颗粒结合淀粉合成酶)活性均呈单峰曲线变化,峰值都出现在20～30 DAP(授粉后天数).2个高淀粉自交系的Q酶(淀粉分支酶)活性也呈单峰曲线变化,峰值也出现在20DAP,而2个低淀粉自交系的Q酶活性则呈双峰曲线变化,2个峰值分别出现在15～20 DAP和30～35DAP.4个自交系籽粒淀粉的积累速率与各自交系ADPG-PPase、SSS和GBSS的活性变化呈极显著正相关.各自交系关键酶活性之间,ADPG-PPase、SSS和GBSS三者间活性变化呈极显著正相关,这3种酶活性变化与Q酶活性变化也呈不同程度的正相关.
이2개고정분화2개저정분옥미자교계위재료,분석료옥미자립정분적동태적루규률,동시대고저정분옥미자립관장과정중정분생물합성관건매활성적동태변화급기여정분적루동태적상관성진행토론분석.연구결과표명:관장과정중4개자교계정분함량변화추세균정sigmoid형곡선.관장과정중ADPG-PPase(선감이린산포도당초린산화매)、SSS(가용성정분합성매)、GBSS(과립결합정분합성매)활성균정단봉곡선변화,봉치도출현재20～30 DAP(수분후천수).2개고정분자교계적Q매(정분분지매)활성야정단봉곡선변화,봉치야출현재20DAP,이2개저정분자교계적Q매활성칙정쌍봉곡선변화,2개봉치분별출현재15～20 DAP화30～35DAP.4개자교계자립정분적적루속솔여각자교계ADPG-PPase、SSS화GBSS적활성변화정겁현저정상관.각자교계관건매활성지간,ADPG-PPase、SSS화GBSS삼자간활성변화정겁현저정상관,저3충매활성변화여Q매활성변화야정불동정도적정상관.
Amylose,amylopectin and starch dynamic accumulation and key enzymes activities in the grains of 4 maize inbred lines (two high-starch ones and two lowstarch ones)were studied.The amounts of amylose.amylopectin and starch in the grains of 4 maize inbred lines increased as sigmoid curves during grain filling period.The changes in amylose,amylopectin and starch accumulation rates followed single-peaked curves,and reached theif peaks in the 25-30 days after pollination (DAP).Changes in activities of adenosine diphosphoglucose pyrophosphorylase(ADPG-PPase,EC 2.7.7.27),soluble starch synthase(sss,EC 2.4.1.21)and starch granule-bound synthase(GBSS,EC 2.4.1.21)in the grains of 4 inbred lines appeared single-peaked curves with the peaks appearing 20-30 DAP.Changes in activities of starch-branching enzyme(Q-enzyme,EC 2.4.1.18) in the grains of high-starch inbred lines appeared single-peaked curves with the peak values at 20 DAP,while the two low-starch inbred lines shows doublepeaked curves with the peak values in the 15-20 DAP and 30-35 DAP.There was significant correlation between ADPG-PPase.SSS and GBSS activities.The results indicated that the Q-enzyme had different expression in high-and low-starch maize inbred lines.and verified that ADPG-PPase,SSS and GBSS activities were significantly and positively correlated with amylose,amylopectin and starch accumulation rates.