CAJ | 학술논문

目的探讨乙型肝炎病毒感染孕妇胎盘中细胞因子的笔表达以及与胎盘细胞凋亡的关系,阐明乙型肝炎病毒感染孕妇致异常妊娠的机理.方法选择30例HBV感染孕妇死胎胎盘作为研究组,正常分娩后的胎盘20例作为对照组,应用免疫组织化学法测定各组胎盘组织中TNF和IL-18的表达,应用TUNEL法检测胎盘细胞凋亡指数. 结果在正常胎盘组织中有一定量的TNF和IL-18的表达,但在HBV感染孕妇死胎胎盘组织中TNF和IL-18的阳性细胞数显著高于对照组(P<0.01);研究组胎盘细胞凋亡指数显著高于对照组(P<0.01),合体滋养细胞的凋亡指数高于滋养细胞,在正常对照组两种细胞的凋亡指数差异有显著性(P<0.05),而在研究组两种细胞的凋亡指数差异无统计学意义(P>0.05).经相关分析发现,胎盘细胞的凋亡指数与TNF、IL-18呈正相关,(A=6.38,B=0.82,r=0.54;A=9.66,B=0.52,r=0.61). 结论 HBV感染孕妇死胎胎盘中TNF和IL-18的表达增加,使胎盘细胞凋亡加速,影响胎盘的正常功能,是导致胎儿异常的原因之一.
목적 탐토을형간염병독감염잉부태반중세포인자적필표체이급여태반세포조망적관계,천명을형간염병독감염잉부치이상임신적궤리.방법 선택30례HBV감염잉부사태태반작위연구조,정상분면후적태반20례작위대조조,응용면역조직화학법측정각조태반조직중TNF화IL-18적표체,응용TUNEL법검측태반세포조망지수. 결과 재정상태반조직중유일정량적TNF화IL-18적표체,단재HBV감염잉부사태태반조직중TNF화IL-18적양성세포수현저고우대조조(P<0.01);연구조태반세포조망지수현저고우대조조(P<0.01),합체자양세포적조망지수고우자양세포,재정상대조조량충세포적조망지수차이유현저성(P<0.05),이재연구조량충세포적조망지수차이무통계학의의(P>0.05).경상관분석발현,태반세포적조망지수여TNF、IL-18정정상관,(A=6.38,B=0.82,r=0.54;A=9.66,B=0.52,r=0.61). 결론 HBV감염잉부사태태반중TNF화IL-18적표체증가,사태반세포조망가속,영향태반적정상공능,시도치태인이상적원인지일.
Objective To investigate the expression of placenta cellular factors in pregnant women infected with hepatitis B virus (HBV) and its relationship with placenta cell apoptosis,and to evaluate the mechanism of the virus resulting in abnormal pregnancy. Methods The cases were divided into two groups: HBV infected stillbirth placental tissue from 30 patients and the normal placental tissue from 20 subjects. Immunohistochemstry was employed to examine the levels of TNF and IL-18 in the tissue and TUNEL assay to determine the placental cell apoptotic index. Results There was a low level of expression of TNF and IL-18 in normal placenta,however the levels of their expression in HBV infected placental tissue were significantly higher than in the control (P<0.01). Meanwhile,placental apoptotic index in HBV infected group was higher than in the control (P<0.01). Its index in syncytiotrophoblast was greater than in trophoblast. Furthermore,there was a significant difference in apoptotic index between both cells in normal control (P<0.05),but no difference in the HBV infected group (P>0.05). There was a positive correlation between apoptotic index and expression of TNF as well as IL-18 (A=6.38,B=0.82,r=0.54;A=9.66,B=0.52,r=0.61). Conclusion In HBV infected stillbirth placenta levels of TNF and IL-18 increase,resulting in a rise in cell apoptosis and impacting placental functions which may lead to the abnormal fetus.