CAJ | 학술논문

[目的]研究短时间不同剂量UV-B辐射对冬小麦幼苗叶片生理指标的影响,为进一步研究植物应答短时间紫外线照射机理提供参考.[方法]以冬小麦为试验材料,采用15和30 μW/cm~2的UV-B照射强度对冬小麦幼苗进行短时间照射处理,并测定色素含量、光合速率等生理指标.[结果]UV-B处理导致叶绿素、可溶性蛋白含量以及含水量都降低,且存在剂量效应,30 μW/cm~2处理的降幅大于15 μW/cm~2;UV-B辐射处理对类胡萝卜素含量的影响与花青素相同,均表现为先下降后上升的趋势,2种剂量间差异不大;2种剂量的UV-B处理均抑制了PS Ⅱ的电子传递,尤其是处理2 h的抑制作用最大,4、6和8 h的抑制作用有所缓解,且存在剂量效应;2种剂量的UV-B辐射均抑制了叶片的光合速率,随着照射时间的延长抑制效应加大;高剂量UV-B处理对膜系统的损伤较大,低剂量UV-B处理结果不明显.[结论]短时间UV-B辐射处理对冬小麦幼苗叶片的影响具有剂量效应,高剂量比低剂量的作用效果大.且随着处理时间的延长,受害程度有所减轻,该结果与长时间UV-B照射的结果不同.
[목적]연구단시간불동제량UV-B복사대동소맥유묘협편생리지표적영향,위진일보연구식물응답단시간자외선조사궤리제공삼고.[방법]이동소맥위시험재료,채용15화30 μW/cm~2적UV-B조사강도대동소맥유묘진행단시간조사처리,병측정색소함량、광합속솔등생리지표.[결과]UV-B처리도치협록소、가용성단백함량이급함수량도강저,차존재제량효응,30 μW/cm~2처리적강폭대우15 μW/cm~2;UV-B복사처리대류호라복소함량적영향여화청소상동,균표현위선하강후상승적추세,2충제량간차이불대;2충제량적UV-B처리균억제료PS Ⅱ적전자전체,우기시처리2 h적억제작용최대,4、6화8 h적억제작용유소완해,차존재제량효응;2충제량적UV-B복사균억제료협편적광합속솔,수착조사시간적연장억제효응가대;고제량UV-B처리대막계통적손상교대,저제량UV-B처리결과불명현.[결론]단시간UV-B복사처리대동소맥유묘협편적영향구유제량효응,고제량비저제량적작용효과대.차수착처리시간적연장,수해정도유소감경,해결과여장시간UV-B조사적결과불동.
[Objective]Studying the influences of different UV-B radiation treatments in short time on some physiological characteristics of water wheat seedlings was to provide reference for the mechanism of plant response under ultraviolet irradiation in short time.[Method] The winter wheat were taken as materials to radiate by 15 and 30 μW/cm2 UV-B,then the physiological indexes such as pigment content and photosynthetic rate were determined.[Results] The UV-B treatment caused the declines of chlorophyll content,soluble protein content and water content of leaves,besides,dose-effect was existed.The decline of 30 μW/cm2 treatment was bigger than that of 15 μW/cm2 treatment.The influences of UV-B radiation on carotenoid content and anthocyanidin content were similar; the change trends were declined firstly then increased.The UV-B treatment with two doses restrained the electron transport of PSⅡ,particularly; the inhibitory effect was biggest after treated 2h,and then this effect was declined in 4,6 and 8 h,so the dose-effect was existed.The UV-B radiation with two doses restricted photosynthetic rate and the inhibitory effect increased with the increase of treatment time.The high dose treatment caused huge damage to membrane system,while the result of low dose treatment was not obvious.[Conclusion] UV-B radiation treatment had dose-effect on winter wheat seedlings in short time and the influence of high dose was bigger than that of low dose.With the increase of treatment time,the damage was alleviated; besides,the result was not similar to that of UV-B radiation in long time.