安徽农业科学
安徽農業科學
안휘농업과학
JOURNAL OF ANHUI AGRICULTURAL SCIENCES
2010年
3期
1171-1172
,共2页
李赵志%张立春%张国梁%严昌国%金海国%曹阳%周国利%王晓阳%朴庆林%于永生%刘晓辉
李趙誌%張立春%張國樑%嚴昌國%金海國%曹暘%週國利%王曉暘%樸慶林%于永生%劉曉輝
리조지%장립춘%장국량%엄창국%금해국%조양%주국리%왕효양%박경림%우영생%류효휘
草原红牛%钙蛋白酶基因%PCR-SSCP
草原紅牛%鈣蛋白酶基因%PCR-SSCP
초원홍우%개단백매기인%PCR-SSCP
Red steppe cattle%CAPN%PCR-SSCP
[目的]利用PCR-SSCP技术研究中国草原红牛CAPN I基因的多态性.[方法]以90头草原红牛的全血为试材,用酚/氯仿/异戊醇法提取基因组DNA,采用PCR-SSCP技术分析了钙蛋白酶 I 基因(CAPN I)在草原红牛群体中的多态性.[结果]试验所设计的18对引物用于PCR扩增均获得良好的结果.经PCR-SSCP 分析,E7-1引物扩增的群体出现了AA、BB、AB和AC 4种基因型;E14-1引物扩增的群体出现了 AA和BB 2种基因型,E14-5、E14-6和 E20-2引物扩增的群体出现了 AA、BB和AB 3种基因型.对不同基因型的测序表明,在第14内含子4 685 bp处发生了C-T的碱基突变,在第17内含子6 545 bp处发生了C-T的碱基突变,在第21内含子上8 462 bp处发生了G-A、8 561 bp处A-G、8 696 bp处A-T的碱基突变.[结论]该研究为改善牛肉质性状的分子育种提供新的分子生物学基础数据.
[目的]利用PCR-SSCP技術研究中國草原紅牛CAPN I基因的多態性.[方法]以90頭草原紅牛的全血為試材,用酚/氯倣/異戊醇法提取基因組DNA,採用PCR-SSCP技術分析瞭鈣蛋白酶 I 基因(CAPN I)在草原紅牛群體中的多態性.[結果]試驗所設計的18對引物用于PCR擴增均穫得良好的結果.經PCR-SSCP 分析,E7-1引物擴增的群體齣現瞭AA、BB、AB和AC 4種基因型;E14-1引物擴增的群體齣現瞭 AA和BB 2種基因型,E14-5、E14-6和 E20-2引物擴增的群體齣現瞭 AA、BB和AB 3種基因型.對不同基因型的測序錶明,在第14內含子4 685 bp處髮生瞭C-T的堿基突變,在第17內含子6 545 bp處髮生瞭C-T的堿基突變,在第21內含子上8 462 bp處髮生瞭G-A、8 561 bp處A-G、8 696 bp處A-T的堿基突變.[結論]該研究為改善牛肉質性狀的分子育種提供新的分子生物學基礎數據.
[목적]이용PCR-SSCP기술연구중국초원홍우CAPN I기인적다태성.[방법]이90두초원홍우적전혈위시재,용분/록방/이무순법제취기인조DNA,채용PCR-SSCP기술분석료개단백매 I 기인(CAPN I)재초원홍우군체중적다태성.[결과]시험소설계적18대인물용우PCR확증균획득량호적결과.경PCR-SSCP 분석,E7-1인물확증적군체출현료AA、BB、AB화AC 4충기인형;E14-1인물확증적군체출현료 AA화BB 2충기인형,E14-5、E14-6화 E20-2인물확증적군체출현료 AA、BB화AB 3충기인형.대불동기인형적측서표명,재제14내함자4 685 bp처발생료C-T적감기돌변,재제17내함자6 545 bp처발생료C-T적감기돌변,재제21내함자상8 462 bp처발생료G-A、8 561 bp처A-G、8 696 bp처A-T적감기돌변.[결론]해연구위개선우육질성상적분자육충제공신적분자생물학기출수거.
[Objective] The aim was to research the polymorphism of polymorphism of calpain I(CAPN I) gene in China Red Steppe by using PCR-SSCP technique. [Method] With the whole blood of 90 head China Red Steppe as the tested material, the genome DNA was extracted by using phenol/chloroform/isoamyl alcohol method and the CAPN I gene in the China Red Steppe group by using PCR-SSCP technique. [Result] The designed 18 pairs of primers used to PCR amplification all got the better results. Through PCR-SSCP analysis, the group of E7-1 primer amplification appeared 4 kinds of genotypes of AA, BB, AB and AC; the group of E14-1 primer amplification appeared 2 kinds of genotypes of AA and BB, the group of E14-5, E14-6, E20-2 primer amplification appeared 2 kinds of genotypes of AA, BB and AB. The sequencing on different genotypes showed that the base mutation of C-T was happened at the 14th intron 4 685 bp, the base mutation of C-T was happened at the 17th intron 6 545 bp, the base mutation of G-A, A-G, A-T were happened at the 21th intron 8 462, 8 561 and 8 696 bp resp. [Conclusion] This research provided the basic data of molecular biology for the molecular breeding of improving the beef quality characteristics.
