CAJ | 학술논문

背景:目前组织工程骨的支架材料,力学性能和体内血管化问题仍没有很好解决,因而制约了临床的广泛开展.目的:观察生物衍生骨体外复合骨髓间充质干细胞构建组织化工程骨修复兔桡骨一骨膜缺损的血管化进程和生物力学性能.方法:取新西兰大白兔的骨头制各生物衍生骨;体外分离和培养兔骨髓间充质干细胞,定向诱导骨髓间充质干细胞向成骨细胞分化,再将诱导的成骨细胞接种到生物衍生骨支架上制成组织工程骨.取大白兔25只,制成骨膜桡骨缺损模型,其中5只兔不做处理为空白组;另外20只兔左前肢为对照组单纯植入生物衍生骨,右前肢为实验组植入组织工程骨术后2,4,8,12周分别取5只兔标本,观察血管面积和缺损区植入骨的生物力学性能.结果与结论:术后4周实验组和对照组血液循环丰富,血管面积较大,12周血管面积趋于稳定,接近正常状态;2,4,8周的实验组和对照组血管面积与空白组比较差异有显著性意义(P<0.05).8周,与对照组比较,实验组修复区的血管网排列规律,12周形态结构已接近正常骨组织,力学性能明显增强.结果证实生物衍生骨复合骨髓间充质干细胞后有较好的血管化进程和力学性能,是修复骨缺损的一种较好的治疗方法.
배경:목전조직공정골적지가재료,역학성능화체내혈관화문제잉몰유흔호해결,인이제약료림상적엄범개전.목적:관찰생물연생골체외복합골수간충질간세포구건조직화공정골수복토뇨골일골막결손적혈관화진정화생물역학성능.방법:취신서란대백토적골두제각생물연생골;체외분리화배양토골수간충질간세포,정향유도골수간충질간세포향성골세포분화,재장유도적성골세포접충도생물연생골지가상제성조직공정골.취대백토25지,제성골막뇨골결손모형,기중5지토불주처리위공백조;령외20지토좌전지위대조조단순식입생물연생골,우전지위실험조식입조직공정골술후2,4,8,12주분별취5지토표본,관찰혈관면적화결손구식입골적생물역학성능.결과여결론:술후4주실험조화대조조혈액순배봉부,혈관면적교대,12주혈관면적추우은정,접근정상상태;2,4,8주적실험조화대조조혈관면적여공백조비교차이유현저성의의(P<0.05).8주,여대조조비교,실험조수복구적혈관망배렬규률,12주형태결구이접근정상골조직,역학성능명현증강.결과증실생물연생골복합골수간충질간세포후유교호적혈관화진정화역학성능,시수복골결손적일충교호적치료방법.
BACKGROUND: The biomechanical property and in vivo vascularization restrict the widely application of tissue engineering bone in clinic.OBJECTIVE: To study the vascularization and biomechanical property of tissue engineered bone in vitro constructed by bio-derived bone combined with bone marrow mesenchymal stem cells (BMSCs) in repair of radius-periosteum defects in rabbits METHODS: The bio-derived bone was prepared using bones of New Zealand white rabbits, and the BMSCs of rabbits were harvested and cultured in osteogenic conditions, followed by incubation into bio-derived bone to prepare tissue engineered bone Twenty-five rabbits were prepared for radius-periosteum defects models. The left forelimbs of rabbits in the control group were repaired by bio-derived bone, and the right forelimbs of rabbits in the experimental group were repaired by tissue engineered bone. There was no treatment in the blank group. Each 5 rabbits were sacrificed at weeks 2, 4, 8 and 12 after operation. The vascular area and biomechanical property of implanted bone were observed.RESULTS AND CONCLUSION: In the experimental and control groups, there were massive blood circulation with large vasculai area at 4 weeks after operation, which trend to normal level at 12 weeks. The differences of vessel sizes between the experimental, control and blank groups had dramatically significance (P < 0.05). Compared with the control group, the vascular network ranged regularly, which similar to normal bone tissues at 12 weeks. The results demonstrated that tissue engineered bone has better vascularization and biomechanics property. It is a better way to repair bone defects.