CAJ | 학술논문

目的:探讨糖尿病肾病(DN)早期大鼠肾组织中硫氧还蛋白相互作用蛋白(Txnip)表达的变化.方法:Wistar大鼠随机分成正常对照组(NC组)、糖尿病模型组(DM组).腹腔注射链脲佐菌素诱导糖尿病模型,4周时取大鼠肾脏,HE染色观察大鼠肾组织的病理变化,免疫组化检测大鼠肾组织中Txnip的表达分布,Western blot和RT-PCR进一步检测Txnip蛋白和基因的表达.结果:在DN早期,与NC组肾组织相比,DM组的肾小管和肾小球中均发现Txnip蛋白和基因的表达增加.结论:Txnip表达的变化在大鼠DN早期发挥重要作用.
목적:탐토당뇨병신병(DN)조기대서신조직중류양환단백상호작용단백(Txnip)표체적변화.방법:Wistar대서수궤분성정상대조조(NC조)、당뇨병모형조(DM조).복강주사련뇨좌균소유도당뇨병모형,4주시취대서신장,HE염색관찰대서신조직적병리변화,면역조화검측대서신조직중Txnip적표체분포,Western blot화RT-PCR진일보검측Txnip단백화기인적표체.결과:재DN조기,여NC조신조직상비,DM조적신소관화신소구중균발현Txnip단백화기인적표체증가.결론:Txnip표체적변화재대서DN조기발휘중요작용.
Objective To investigate expression of thioredoxin interacting protein (Txnip) in rat kidneys in the early stage of diabetic nephropathy. Methods Diabetic model rats were induced by intraperitoneal injection of STZ. Rats were divided into normal group and DM model group. In the fourth week after the STZ treatment, histopathological changes of rat kidneys were observed by HE staining, and Txnip was detected by immunohistochemical technology. Furthermore protein and mRNA expression of Txnip was analyzed by Western blot and RT-PCR separately. Results Immunohistochemical stain showed that Txnip protein located in the renal tubule and glomeruli of diabetic rats. The levels of Txnip protein and mRNA were significantly increased in DM group as compared with normal group. Conclusions Up-regulation of Txnip expression is associated with the early stage of diabetic nephropathy in rats.