国际中医中药杂志
國際中醫中藥雜誌
국제중의중약잡지
INTERNATIONAL JOURNAL OF TRIDITIONAL CHINESE MEDICINE
2010年
4期
293-295
,共3页
肝纤维化%扶正理气合剂%肝星状细胞%脂质过氧化
肝纖維化%扶正理氣閤劑%肝星狀細胞%脂質過氧化
간섬유화%부정리기합제%간성상세포%지질과양화
Hepatic fibrosis%Fuzheng-Liqi mixture%Hepatic stellate cell%Lipid peroxidation
目的 探讨复方中药扶正理气合剂对洒精性肝纤维化的预防机制和效果.方法 雄性SD大鼠80只,随机分为正常组、模型组、复方丹参组和扶正理气合剂组.以乙醇灌胃诱导大鼠肝纤维化模型,复方丹参组造模同时给予复方丹参灌胃,扶正理气合剂组造模同时给予扶正理气合剂灌胃,共16周.16周后处死大鼠取肝组织标本,光镜观察肝组织的病理变化,放射免疫法测定血清丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性.结果 ①模型组多数正常小叶结构破坏或消失,由汇管区和中央静脉伸出粗大胶原纤维条索分割肝小叶,肝细胞索排列紊乱,肝细胞明显水肿,广泛脂肪变性.纤维隔内有大量淋巴细胞、单核细胞、嗜酸性粒细胞等浸润.与模型组比较,两个用药组肝小叶结构破坏明显减轻,肝脏胶原纤维增生亦明显减轻,纤维条索疏松变窄.肝细胞水肿好转,炎细胞浸润减少.②Masson染色胶原纤维面积比较:模型组胶原面积比正常组显著增加(P＜0.05),复方丹参组和扶正理气合剂组胶原面积较模型组均减轻(P＜0.05),扶正理气合剂组与复方丹参组比较无显著差异(P＞0.05).③与模型组比较,复方丹参组、扶正理气合剂组MDA下降(P＜0.01)、SOD升高(P＜0.05).结论 扶正理气合剂抗纤维化作用可能与其抗脂质过氧化作用有关.
目的 探討複方中藥扶正理氣閤劑對灑精性肝纖維化的預防機製和效果.方法 雄性SD大鼠80隻,隨機分為正常組、模型組、複方丹參組和扶正理氣閤劑組.以乙醇灌胃誘導大鼠肝纖維化模型,複方丹參組造模同時給予複方丹參灌胃,扶正理氣閤劑組造模同時給予扶正理氣閤劑灌胃,共16週.16週後處死大鼠取肝組織標本,光鏡觀察肝組織的病理變化,放射免疫法測定血清丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性.結果 ①模型組多數正常小葉結構破壞或消失,由彙管區和中央靜脈伸齣粗大膠原纖維條索分割肝小葉,肝細胞索排列紊亂,肝細胞明顯水腫,廣汎脂肪變性.纖維隔內有大量淋巴細胞、單覈細胞、嗜痠性粒細胞等浸潤.與模型組比較,兩箇用藥組肝小葉結構破壞明顯減輕,肝髒膠原纖維增生亦明顯減輕,纖維條索疏鬆變窄.肝細胞水腫好轉,炎細胞浸潤減少.②Masson染色膠原纖維麵積比較:模型組膠原麵積比正常組顯著增加(P＜0.05),複方丹參組和扶正理氣閤劑組膠原麵積較模型組均減輕(P＜0.05),扶正理氣閤劑組與複方丹參組比較無顯著差異(P＞0.05).③與模型組比較,複方丹參組、扶正理氣閤劑組MDA下降(P＜0.01)、SOD升高(P＜0.05).結論 扶正理氣閤劑抗纖維化作用可能與其抗脂質過氧化作用有關.
목적 탐토복방중약부정리기합제대쇄정성간섬유화적예방궤제화효과.방법 웅성SD대서80지,수궤분위정상조、모형조、복방단삼조화부정리기합제조.이을순관위유도대서간섬유화모형,복방단삼조조모동시급여복방단삼관위,부정리기합제조조모동시급여부정리기합제관위,공16주.16주후처사대서취간조직표본,광경관찰간조직적병리변화,방사면역법측정혈청병이철(MDA)함량화초양화물기화매(SOD)활성.결과 ①모형조다수정상소협결구파배혹소실,유회관구화중앙정맥신출조대효원섬유조색분할간소협,간세포색배렬문란,간세포명현수종,엄범지방변성.섬유격내유대량림파세포、단핵세포、기산성립세포등침윤.여모형조비교,량개용약조간소협결구파배명현감경,간장효원섬유증생역명현감경,섬유조색소송변착.간세포수종호전,염세포침윤감소.②Masson염색효원섬유면적비교:모형조효원면적비정상조현저증가(P＜0.05),복방단삼조화부정리기합제조효원면적교모형조균감경(P＜0.05),부정리기합제조여복방단삼조비교무현저차이(P＞0.05).③여모형조비교,복방단삼조、부정리기합제조MDA하강(P＜0.01)、SOD승고(P＜0.05).결론 부정리기합제항섬유화작용가능여기항지질과양화작용유관.
Objective To explore the mechanisms and preventive effects of Fuzheng-Liqi mixture on alcoholic hepatic fibrosis. Methods 48 male SD rats were randomly divided into four groups: normal group, model group, Compound Salviae Miltiorrhizae (CSM) group and Fuzheng-Liqi mixture (FLM) group. Alcohol was poured into stomach of the rats for 16 weeks to induce hepatic fibrosis. At the same time, CSM and FLM were given to each group. The rats were killed after 16 weeks. Histomorphylogic structure of the liver tissues was observed under optical microscope; the levels of MDA and SOD in rounded by thick cords of collagen fibers, which extended from the portal areas and central veins. Obvious cloudy swelling could be seen in hepatocytes. Fatty degeneration was extensive, and some with necroses fiber septa were infiltrated by a great number of cells such as monocytes, lymphocytes, eosinophilic granulocytes, fibroblasts, and so on. Compared with the model group, the destructions were slighter, proliferations of collagen fibers were also fewer, fiber cords were more loosened and comparison of collagen area in Masson staining: The collagen area in the CSM group and FLM group were less than the model of serum SOD was increased obviously in the FLM group (P＜0.05). Conclusion The mechanism of Fuzheng-Liqi mixture in anti-fibrosis effects may be correlated with its anti-lipid peroxidation.
