国际生物医学工程杂志
國際生物醫學工程雜誌
국제생물의학공정잡지
INTERNATIONAL JOURNAL OF BIOMEDICAL ENGINEERING
2011年
6期
336-339
,共4页
俞燚%刘肖珩%沈阳%高亭%高敏%马云龙%付强%康敏%赵健民
俞燚%劉肖珩%瀋暘%高亭%高敏%馬雲龍%付彊%康敏%趙健民
유일%류초형%침양%고정%고민%마운룡%부강%강민%조건민
白细胞介素-8%血管内皮细胞%紧密连接
白細胞介素-8%血管內皮細胞%緊密連接
백세포개소-8%혈관내피세포%긴밀련접
Interleukin-8%Vascular endothelial cell%Tight junction
目的 研究白细胞介素-8(IL-8)对血管内皮细胞紧密连接的影响.方法 采用免疫荧光染色检测经不同浓度和时间IL-8处理后EA.hy926细胞的3种紧密连接蛋白occludin、claudin-5和ZO-1的形态和分布；逆转录PCR(RT-PCR)检测3种蛋白mRNA的表达水平.结果 IL-8可改变内皮细胞紧密连接蛋白occludin、claudin-5和ZO-1的分布和表达强度；随着IL-8浓度和作用时间的增加,occludin、claudin-5和ZO-1 mRNA表达逐渐降低.结论 IL-8改变occludin、claudin-5和ZO-13种紧密连接蛋白的分布和表达,并下调其mRNA表达,且呈现出一定的时间和浓度依赖关系.
目的 研究白細胞介素-8(IL-8)對血管內皮細胞緊密連接的影響.方法 採用免疫熒光染色檢測經不同濃度和時間IL-8處理後EA.hy926細胞的3種緊密連接蛋白occludin、claudin-5和ZO-1的形態和分佈；逆轉錄PCR(RT-PCR)檢測3種蛋白mRNA的錶達水平.結果 IL-8可改變內皮細胞緊密連接蛋白occludin、claudin-5和ZO-1的分佈和錶達彊度；隨著IL-8濃度和作用時間的增加,occludin、claudin-5和ZO-1 mRNA錶達逐漸降低.結論 IL-8改變occludin、claudin-5和ZO-13種緊密連接蛋白的分佈和錶達,併下調其mRNA錶達,且呈現齣一定的時間和濃度依賴關繫.
목적 연구백세포개소-8(IL-8)대혈관내피세포긴밀련접적영향.방법 채용면역형광염색검측경불동농도화시간IL-8처리후EA.hy926세포적3충긴밀련접단백occludin、claudin-5화ZO-1적형태화분포；역전록PCR(RT-PCR)검측3충단백mRNA적표체수평.결과 IL-8가개변내피세포긴밀련접단백occludin、claudin-5화ZO-1적분포화표체강도；수착IL-8농도화작용시간적증가,occludin、claudin-5화ZO-1 mRNA표체축점강저.결론 IL-8개변occludin、claudin-5화ZO-13충긴밀련접단백적분포화표체,병하조기mRNA표체,차정현출일정적시간화농도의뢰관계.
Objective To investigate the influence of IL-8 on the tight junction of vascular endothelial cells.Methods Immunofluorescence was used to observe the modality and the distribution of three tight junction proteins (occludin,claudin-5 and ZO-1) of the EA.hy926 cells treated with IL-8 under different concentrations and different times.RT-PCR was used to measure the mRNA expression of these three proteins.Results The results demonstrated that IL-8 could change the distribution of occludin,claudin-5 and ZO-1 in EA.hy926 cells,and the mRNA expression of occludin,claudin-5 and ZO-1 decreased with the increase of IL-8 concentration and treated time.Conclusion The effects of IL-8 on the distribution and the expression of occludin,claudin-5 and ZO-1 are dose and time-dependent.
