国际呼吸杂志
國際呼吸雜誌
국제호흡잡지
INTERNATIONAL JOURNAL OF RESPIRATION
2011年
17期
1298-1302
,共5页
晏斌林%吴肖叶%陈和平%何明
晏斌林%吳肖葉%陳和平%何明
안빈림%오초협%진화평%하명
支气管哮喘%哮喘模型%气管上皮%阴离子交换蛋白2
支氣管哮喘%哮喘模型%氣管上皮%陰離子交換蛋白2
지기관효천%효천모형%기관상피%음리자교환단백2
Bronchial asthma%Asthma model%Airway epithelium%Anion exchanger 2
目的 研究支气管哮喘（简称哮喘）豚鼠气道上皮细胞阴离子交换蛋白2( anion exchanger2，AE2)的表达及意义。方法取20只豚鼠随机分为2组，每组用卵清白蛋白(OVA)致敏，然后分别用生理盐水（对照组）和OVA(哮喘组)雾化吸入激发豚鼠哮喘。在连续雾化3d后，刮取气道上皮以免疫印迹( western blotting)和逆转录-聚合酶链反应(RT-PCR)法作AE2蛋白及其mRNA表达水平的检测。结果①呼吸频率：激发后对照组与哮喘组比较差异有统计学意义(P＜0.01)；同组内激发前后比较对照组内差异无统计学意义(P＞0.05)，哮喘组内比较差异有统计学意义(P＜0.01)。②血清IgE浓度和血嗜酸粒细胞(EOS)计数：对照组IgE浓度和EOS计数与哮喘组比较，差异有统计学意义(P＜0.01)。③豚鼠肺组织病理形态改变：光学显微镜观察发现：对照组，豚鼠肺组织未见病理形态改变，哮喘组则有明显哮喘病理形态改变。④AE2蛋白及其mRNA的表达：结果表明对照组豚鼠气道上皮有较高的AE2蛋白和mRNA表达量，哮喘组相对对照组表达明显降低，差异有统计学意义(P＜0.01)。结论 AE2蛋白及mRNA在正常豚鼠气道上皮有一定的基础表达。过敏性哮喘模型豚鼠气道上皮细胞表达的AE2蛋白及mRNA表达明显减少。
目的 研究支氣管哮喘（簡稱哮喘）豚鼠氣道上皮細胞陰離子交換蛋白2( anion exchanger2，AE2)的錶達及意義。方法取20隻豚鼠隨機分為2組，每組用卵清白蛋白(OVA)緻敏，然後分彆用生理鹽水（對照組）和OVA(哮喘組)霧化吸入激髮豚鼠哮喘。在連續霧化3d後，颳取氣道上皮以免疫印跡( western blotting)和逆轉錄-聚閤酶鏈反應(RT-PCR)法作AE2蛋白及其mRNA錶達水平的檢測。結果①呼吸頻率：激髮後對照組與哮喘組比較差異有統計學意義(P＜0.01)；同組內激髮前後比較對照組內差異無統計學意義(P＞0.05)，哮喘組內比較差異有統計學意義(P＜0.01)。②血清IgE濃度和血嗜痠粒細胞(EOS)計數：對照組IgE濃度和EOS計數與哮喘組比較，差異有統計學意義(P＜0.01)。③豚鼠肺組織病理形態改變：光學顯微鏡觀察髮現：對照組，豚鼠肺組織未見病理形態改變，哮喘組則有明顯哮喘病理形態改變。④AE2蛋白及其mRNA的錶達：結果錶明對照組豚鼠氣道上皮有較高的AE2蛋白和mRNA錶達量，哮喘組相對對照組錶達明顯降低，差異有統計學意義(P＜0.01)。結論 AE2蛋白及mRNA在正常豚鼠氣道上皮有一定的基礎錶達。過敏性哮喘模型豚鼠氣道上皮細胞錶達的AE2蛋白及mRNA錶達明顯減少。
목적 연구지기관효천（간칭효천）돈서기도상피세포음리자교환단백2( anion exchanger2，AE2)적표체급의의。방법취20지돈서수궤분위2조，매조용란청백단백(OVA)치민，연후분별용생리염수（대조조）화OVA(효천조)무화흡입격발돈서효천。재련속무화3d후，괄취기도상피이면역인적( western blotting)화역전록-취합매련반응(RT-PCR)법작AE2단백급기mRNA표체수평적검측。결과①호흡빈솔：격발후대조조여효천조비교차이유통계학의의(P＜0.01)；동조내격발전후비교대조조내차이무통계학의의(P＞0.05)，효천조내비교차이유통계학의의(P＜0.01)。②혈청IgE농도화혈기산립세포(EOS)계수：대조조IgE농도화EOS계수여효천조비교，차이유통계학의의(P＜0.01)。③돈서폐조직병리형태개변：광학현미경관찰발현：대조조，돈서폐조직미견병리형태개변，효천조칙유명현효천병리형태개변。④AE2단백급기mRNA적표체：결과표명대조조돈서기도상피유교고적AE2단백화mRNA표체량，효천조상대대조조표체명현강저，차이유통계학의의(P＜0.01)。결론 AE2단백급mRNA재정상돈서기도상피유일정적기출표체。과민성효천모형돈서기도상피세포표체적AE2단백급mRNA표체명현감소。
Objective To the study expression and significance of AE2 protein and mRNA inepithelium of asthmatic guinea pigs. Methods Twenty guinea pigs were randomly divided into control group and asthma group, with 10 guinea pigs per group. The asthmatic model of guinea pigs were established by immunization with intramuscular injected ovalbumin(OVA). The bronchial provocation test by OVA was used in each group,and asthma models were made after consecutive 3 d challenge. To scrape the epithelium in airway of guinea pig and examine the levels of AE2 protein and mRNA by western blotting and RT-PCR technique. Results ① The detection of respiratory frequency: the control group relatively had a significant difference ( P ＜0.01) with the asthma group after challenge and had no significant difference ( P ＞0.05) in the same control group, the asthma group relatively has a significant difference ( P ＜0.01). ②The detection of serum IgE and count eosinophil of blood:compared with the concentration and count of IgE of the control group, the asthma group had significant difference ( P ＜0.01). ③Pulmonary tissue pathologically morphological change:the control group, guinea pig lung tissue slices had no pathologically morphological change. But the asthma group had a distinct picture. ④The levels of AE2 protein and mRNA:the AE2 protein and mRNA appeared a basic expression in the control group. AE2 protein and mRNA expression of the asthma group decreased obviously compared with the control group ( P ＜ 0.001 ). Conclusions AE2 protein and mRNA appeared their basic expression in normal guinea pigs. But expression of AE2 protein and mRNA in asthma group downregulated.
