中国实用护理杂志
中國實用護理雜誌
중국실용호리잡지
CHINESE JOURNAL OF PRACTICAL NURSING
2012年
27期
1-4
,共4页
何良爱%何良燕%张燕%李玉
何良愛%何良燕%張燕%李玉
하량애%하량연%장연%리옥
消毒剂%诱导%外排泵
消毒劑%誘導%外排泵
소독제%유도%외배빙
Disinfectant%Induce%Efflux pump
目的 探讨铜绿假单胞菌在体外经消毒剂诱导后,对抗生素最低抑菌浓度的变化,并对变化机制进行初步研究.方法 收集临床上铜绿假单胞菌菌株68株,采用VITEK-2全自动细菌分析仪鉴定并进行药敏分析；琼脂二倍稀释法分别测定碘伏、戊二醛、氯己定对铜绿假单胞菌的最低抑菌浓度(MIC)；在低于MIC浓度下逐步诱导8次,微量稀释法测定诱导前后铜绿假单胞菌对12种抗生素的MIC,同时再将诱导后对抗生素MIC升高的菌株,接种在含有外排泵抑制剂PAβN的琼脂平板,测定其外排泵表型.结果 68株铜绿假单胞菌经3种消毒剂诱导后共有6株铜绿假单胞菌对一种或多种抗生素的敏感性较诱导前有明显变化,对氟喹诺酮类、头孢类及碳氢酶烯类等多种抗生素MIC升高,碘伏及戊二醛诱导效果明显,两种消毒剂诱导后的5种菌株外排泵表型测定为阳性.结论 消毒剂可以诱导某些铜绿假单胞菌对抗生素的敏感性发生变化,其中主动外排系统在诱导过程中起主要作用,应合理使用消毒剂,以降低耐药菌扩散及医院感染的发生.
目的 探討銅綠假單胞菌在體外經消毒劑誘導後,對抗生素最低抑菌濃度的變化,併對變化機製進行初步研究.方法 收集臨床上銅綠假單胞菌菌株68株,採用VITEK-2全自動細菌分析儀鑒定併進行藥敏分析；瓊脂二倍稀釋法分彆測定碘伏、戊二醛、氯己定對銅綠假單胞菌的最低抑菌濃度(MIC)；在低于MIC濃度下逐步誘導8次,微量稀釋法測定誘導前後銅綠假單胞菌對12種抗生素的MIC,同時再將誘導後對抗生素MIC升高的菌株,接種在含有外排泵抑製劑PAβN的瓊脂平闆,測定其外排泵錶型.結果 68株銅綠假單胞菌經3種消毒劑誘導後共有6株銅綠假單胞菌對一種或多種抗生素的敏感性較誘導前有明顯變化,對氟喹諾酮類、頭孢類及碳氫酶烯類等多種抗生素MIC升高,碘伏及戊二醛誘導效果明顯,兩種消毒劑誘導後的5種菌株外排泵錶型測定為暘性.結論 消毒劑可以誘導某些銅綠假單胞菌對抗生素的敏感性髮生變化,其中主動外排繫統在誘導過程中起主要作用,應閤理使用消毒劑,以降低耐藥菌擴散及醫院感染的髮生.
목적 탐토동록가단포균재체외경소독제유도후,대항생소최저억균농도적변화,병대변화궤제진행초보연구.방법 수집림상상동록가단포균균주68주,채용VITEK-2전자동세균분석의감정병진행약민분석；경지이배희석법분별측정전복、무이철、록기정대동록가단포균적최저억균농도(MIC)；재저우MIC농도하축보유도8차,미량희석법측정유도전후동록가단포균대12충항생소적MIC,동시재장유도후대항생소MIC승고적균주,접충재함유외배빙억제제PAβN적경지평판,측정기외배빙표형.결과 68주동록가단포균경3충소독제유도후공유6주동록가단포균대일충혹다충항생소적민감성교유도전유명현변화,대불규낙동류、두포류급탄경매희류등다충항생소MIC승고,전복급무이철유도효과명현,량충소독제유도후적5충균주외배빙표형측정위양성.결론 소독제가이유도모사동록가단포균대항생소적민감성발생변화,기중주동외배계통재유도과정중기주요작용,응합리사용소독제,이강저내약균확산급의원감염적발생.
Objective To investigate the minimal inhibitory concentration (MIC) of Pseudomonas aeruginosa to antibacterial agents which were induced by disinfectants.Methods The susceptibility test of 68 strains from clinic was determined by VITEK-2 system.The MIC of povidone iodine,glutaraldehyde and chlorhexidine was determined by agar dilution method.68 strains were induced by different concentrations of three kinds of disinfectants for eight times.The concentration of three kinds of disinfectants was lower than their MIC.The MIC before and after inducement was determined by micro dilution.In the mean-while,strains of their MIC raised to antibiotics were inoculated on plate agar containing the PAβN which was the efflux pump inhibitor,in order to determine the phenotype of efflux pump.Results Six strains,MIC in 68 strains significantly changed after induction of disinfectants.The MIC of these six strains was higher than pre-induced to some antibacterial agents such as FQNs,cephems and carbapenem.The induction of povidone iodine and gilutaraldehyde were more obvious,and five strains,phenotype of efflux pump were positive after being induced by these two disinfectants.Conclusions The antimicrobial susceptibility of some Pseu-domonas aeruginosa will change after being induced by some disinfectants and the efflux pump will play an important role in this process.So the reasonable application of disinfectants were indispensable,in order to decrease the resistance and the opportunity of nosocomial infection.
