中华流行病学杂志
中華流行病學雜誌
중화류행병학잡지
CHINESE JOURNAL OF EPIDEMIOLOGY
2009年
3期
231-234
,共4页
赵飞%王哲%朱谦%崔兆麟
趙飛%王哲%硃謙%崔兆麟
조비%왕철%주겸%최조린
人免疫缺陷病毒1型%亚型%巢式聚合酶链反应%序列测定
人免疫缺陷病毒1型%亞型%巢式聚閤酶鏈反應%序列測定
인면역결함병독1형%아형%소식취합매련반응%서렬측정
Human immunodeficiency virus type 1%Subtype%Nested PCR%Sequence analysis
目的 了解河南省人免疫缺陷病毒1型(HIV-1)毒株的亚型状况及序列变异、流行特征,分析其传播来源和传播规律.方法 对2006-2007年在河南省采集的1287例HIV-1感染者抽取静脉血,用巢式聚合酶链反应(nest-PCR)对单个核细胞中前病毒脱氧核糖核酸的env基因和gag基因进行扩增,并测定和分析核苷酸序列.结果 1287份样本中存在B'、C亚型及BC、AE两个重组亚型,其在所有分析样本中的比例分别为95.882%(1234/1287)、0.466%(6/1287)、2.875%(37/1287)、0.777%(10/1287).与国内及国际的参考毒株RL42、C.95in21068、07-BC.CN.97.C54A、01AE.TH.90.CM240间的离散率分别为(9.327±0.245)%、(5.214±0.183)%、(6.278±0.194)%、(5.332±0.1 58)%.结论 目前河南省艾滋病感染者中有B'、C亚型及BC、AE两个重组亚型.泰国B'亚型依然占主导地位.B'亚型以既往有偿献血人员为主,BC亚型以性传播为主,AE亚型以性传播及既往献血途径为主,C亚型以性传播为主.
目的 瞭解河南省人免疫缺陷病毒1型(HIV-1)毒株的亞型狀況及序列變異、流行特徵,分析其傳播來源和傳播規律.方法 對2006-2007年在河南省採集的1287例HIV-1感染者抽取靜脈血,用巢式聚閤酶鏈反應(nest-PCR)對單箇覈細胞中前病毒脫氧覈糖覈痠的env基因和gag基因進行擴增,併測定和分析覈苷痠序列.結果 1287份樣本中存在B'、C亞型及BC、AE兩箇重組亞型,其在所有分析樣本中的比例分彆為95.882%(1234/1287)、0.466%(6/1287)、2.875%(37/1287)、0.777%(10/1287).與國內及國際的參攷毒株RL42、C.95in21068、07-BC.CN.97.C54A、01AE.TH.90.CM240間的離散率分彆為(9.327±0.245)%、(5.214±0.183)%、(6.278±0.194)%、(5.332±0.1 58)%.結論 目前河南省艾滋病感染者中有B'、C亞型及BC、AE兩箇重組亞型.泰國B'亞型依然佔主導地位.B'亞型以既往有償獻血人員為主,BC亞型以性傳播為主,AE亞型以性傳播及既往獻血途徑為主,C亞型以性傳播為主.
목적 료해하남성인면역결함병독1형(HIV-1)독주적아형상황급서렬변이、류행특정,분석기전파래원화전파규률.방법 대2006-2007년재하남성채집적1287례HIV-1감염자추취정맥혈,용소식취합매련반응(nest-PCR)대단개핵세포중전병독탈양핵당핵산적env기인화gag기인진행확증,병측정화분석핵감산서렬.결과 1287빈양본중존재B'、C아형급BC、AE량개중조아형,기재소유분석양본중적비례분별위95.882%(1234/1287)、0.466%(6/1287)、2.875%(37/1287)、0.777%(10/1287).여국내급국제적삼고독주RL42、C.95in21068、07-BC.CN.97.C54A、01AE.TH.90.CM240간적리산솔분별위(9.327±0.245)%、(5.214±0.183)%、(6.278±0.194)%、(5.332±0.1 58)%.결론 목전하남성애자병감염자중유B'、C아형급BC、AE량개중조아형.태국B'아형의연점주도지위.B'아형이기왕유상헌혈인원위주,BC아형이성전파위주,AE아형이성전파급기왕헌혈도경위주,C아형이성전파위주.
Objective To study the epidemic status of human immunodeficiency virus type 1 (HIV-1) subtypes and sequence variation in Henan thus to explore the characteristics and sources of transmission. Methods HIV-1 env and gag gent were amplified by nested PCR from uncultured peripheral blood mononuclear cells (PBMCs) obtained from 1287 HIV-1 carriers confirmed in Henan from 2006 to 2007. 1287 env and gag genes were obtained from the patients. Results Among 1287 samples, there were 4 HIV-1 strains including subtype B', C and recombinant subtype BC and AE, accounting for 95.882%( 1234/ 1287), 0.466% (6/1287), 2.875% (37/1287), 0.777% (10/1287) respectively. In comparison with the sequence of the international strains of RLA2, C.95in21068, 07-BC.CN.97.C54A, 01AE.TH.90.CM240, the genetic divergence was 9.327%±0.245%, 5.214%±0.183%, 6.278%±0.194% and 5.332%±0.158%, respectively. Conclusion There were 4 HIV-1 strains including subtype B' , C and recombinant subtype BC and AE in Henan with main dominant subtype as subtype B' which was closely related to HIV-1 strains of Thailand B'. The major transmit route in subtype B' was through blood donation in the past years while with BC, it was through sexual transmission. The major transmit routes in subtype AE were sexual transmission and blood donation. The major route of transmission in subtype C was through sexual contact.
