CAJ | 학술논문

目的观察缺血再灌注损伤对肝脏免疫原性的影响.方法 30只大鼠均分为3组,利用免疫荧光及Western blol检测缺血30min再灌注组及缺血60min再灌注组及对照组的肝脏中共刺激分子B7蛋白的表达水平.结果正常肝脏B7-1、B7-2表达处于极低水平(0.035±0.005、0.025±0.004),缺血再灌注后肝脏B7-1、B7-2的蛋白表达明显升高(P<0.01),缺血再灌注60min组的B7-1、B7-2的表达水平明显高于缺血30min再灌注组(B7-1:0.070±0.017比0.051±0.008,B7-2:0.042±0.004.比0.037±0.004,P<0.01).结论缺血再灌注损伤使共刺激分子B7蛋白表达升高,提示缺血再灌注损伤可使移植肝免疫原性升高,这些均能促进急性排斥反应的发生.
목적 관찰결혈재관주손상대간장면역원성적영향.방법 30지대서균분위3조,이용면역형광급Western blol검측결혈30min재관주조급결혈60min재관주조급대조조적간장중공자격분자B7단백적표체수평.결과 정상간장B7-1、B7-2표체처우겁저수평(0.035±0.005、0.025±0.004),결혈재관주후간장B7-1、B7-2적단백표체명현승고(P<0.01),결혈재관주60min조적B7-1、B7-2적표체수평명현고우결혈30min재관주조(B7-1:0.070±0.017비0.051±0.008,B7-2:0.042±0.004.비0.037±0.004,P<0.01).결론 결혈재관주손상사공자격분자B7단백표체승고,제시결혈재관주손상가사이식간면역원성승고,저사균능촉진급성배척반응적발생.
Objective To investigate the effect of ischemia/reperfusion (I/R) injury on immunogenicity of the liver. Methods Thirty rats were randomly divided into three groups. The levels of B7 were assayed by using immunochemistry and Western blotting in the 30 min I/R group, 60-min I/R group, and control group. Results The levels of B7-1 and B7-2 were very low in normal liver tissues (0.035 ± 0. 005, 0. 025 ± 0. 004) , but markedly increased after I/R injury ( P < 0.01). The levels of B7-1 and B7-2 in the 60 min I/R group were higher than those in the 30 min I/R group (B7-1:0. 070 ± 0. 017 vs 0. 051 ± 0. 008, B7-2:0. 042 ± 0.004 vs 0. 037 ± 0.004; both P<0.01). Conclusion I/R injury increased the immunogenicity of the liver by up-regulating the costimulatory molecule B7, promoting the development of acute rejection.