CAJ | 학술논문

目的在整体水平上揭示椎间盘生长期和发育成熟期蛋白的表达及差异.方法对24周胎儿,25和30岁人椎间盘组织进行shotgun蛋白质组学技术分析.提取椎间盘组织蛋白,十二烷基磺酸钠-聚丙烯酰胺凝胶电泳结合反向高效液相色谱串联质谱shotgun蛋白质组学技术分析.使用IPI蛋白数据库与基因本体论(GOA)进行蛋白识别及生物信息分析.结果 24周胎儿.25和30岁共识别的非冗余蛋白分别为524,181和172种;仅在胎儿中识别的高质量蛋白有174种,25和30岁共有但胎儿中未识别的高质量蛋白有20种.3个样品的生物化学特性趋势相似.结论使用shotgun蛋白质组学技术可展示胎儿和成人椎间盘的蛋白质表达谱,揭示生长期和发育成熟期蛋白表达的数量、种类及其差异.
목적 재정체수평상게시추간반생장기화발육성숙기단백적표체급차이.방법 대24주태인,25화30세인추간반조직진행shotgun단백질조학기술분석.제취추간반조직단백,십이완기광산납-취병희선알응효전영결합반향고효액상색보천련질보shotgun단백질조학기술분석.사용IPI단백수거고여기인본체론(GOA)진행단백식별급생물신식분석.결과 24주태인.25화30세공식별적비용여단백분별위524,181화172충;부재태인중식별적고질량단백유174충,25화30세공유단태인중미식별적고질량단백유20충.3개양품적생물화학특성추세상사.결론 사용shotgun단백질조학기술가전시태인화성인추간반적단백질표체보,게시생장기화발육성숙기단백표체적수량、충류급기차이.
Objective To acquire information on the IVD (intervertebral disc) proteome and analyze the differences of identified proteins during IVD development and maturation by a shotgun proteomics approach so as to identify the global protein expression patterns of IVD tissues from fetus and adults.Methods A 24-week fetus, a 25- and a 30-year-old adult IVD samples were collected and SDS-PAGE,RP-HPLC MS/MS shotgun analyses were performed. Bioinformational analysis with International Protein Index (IPI) database and functional classification with Gene Ontology Annotation (GOA) database were used to evaluate the results. Results A total of 524 proteins were identified in fetal IVD sample while 181and 172 proteins were observed in 25 and 30-year-old samples respectively. Fotty-eight proteins existed in three samples while 84 proteins in the 25-years-old and 30-years-old samples but not in fetus. Only 174high-quality proteins existed in fetal sample while 20 high-quality proteins in 25-year-old and 30-year-old samples. The physico-chemical characteristics of identified proteins displayed similar trends in three samples. Conclusions This study represents the first presentation of a global proteomic map of fetal and adult IVD samples using shotgun technology. Substantial differences exist in number and variety of proteins between development and mature IVD. This contributes to our overall knowledge in of biochemical components, metabolic regulation and biological mechanics in IVD.