中华眼底病杂志
中華眼底病雜誌
중화안저병잡지
CHINESE JOURNAL OF OCULAR FUNDUS DISEASES
2011年
3期
250-254
,共5页
糖尿病视网膜病变/病理生理学%内皮,血管%造血干细胞%糖尿病,实验性
糖尿病視網膜病變/病理生理學%內皮,血管%造血榦細胞%糖尿病,實驗性
당뇨병시망막병변/병리생이학%내피,혈관%조혈간세포%당뇨병,실험성
Diabetic retinopathy/pathophysiology%Endothelium,vascular%Myeloid progenitor cells%Diabetes mellitus,experimental
目的 观察糖尿病视网膜病变(DR)对循环外周血内皮祖细胞(EPCs)的影响.方法 雄性成年Wistar大鼠60只,随机分为正常对照组和糖尿病组.后者通过腹腔注射链脲佐菌素(STZ)建立DR模型.采用流式细胞仪分别计数建模后1周、1、3、6个月各组大鼠循环外周血EPCs数量,同时于各相应时间点摘除大鼠眼球行苏木精一伊红(HE)染色、视网膜血管消化铺片过碘酸-Schiff(PAS)染色,通过光学显微镜以及透射电子显微镜进行视网膜组织病理学检查.对比分析EPCs数量以及EPCs数量与DR形态学变化的相互关系.结果 大鼠注射STZ 1周、1、3、6个月后循环外周血EPCs数量分别为(25±7)、(28±8)、(39±7)、(43±7)个/20万个单个核细胞,对照组为(45±4)个120万个单个核细胞.与正常对照组大鼠相比,糖尿病组EPCs数量均降低(F=8.933,P＜0.01).随着观察时间的延长,糖尿病组EPCs数量逐渐回升,而视网膜结构、光感受器细胞、血管也逐渐出现病理性改变.光学显微镜下,与正常对照组比较,大鼠注射STZ后1周和1个月组视网膜各层结构清晰完整,但厚度开始变薄,1个月组视网膜细胞排列紊乱,细胞核肿胀、体积增大,光感受器细胞减少,随病程时间增加这些改变逐渐加重,3、6个月组视网膜厚度更薄,细胞排列紊乱更加明显,部分扩张的血管有向外突出、破溃的趋势.透射电子显微镜下,毛细血管内皮细胞水肿,线粒体肿胀;毛细血管基底膜增厚,管腔狭窄,6个月组甚至出现管腔闭塞和视网膜微动脉瘤;光感受器细胞膜盘间隙扩大,排列紊乱,线粒体水肿,细胞早期空泡变性.结论 糖尿病大鼠外周血EPCs数量较正常大鼠降低.随着DR的进展,EPCs数量逐渐回升.
目的 觀察糖尿病視網膜病變(DR)對循環外週血內皮祖細胞(EPCs)的影響.方法 雄性成年Wistar大鼠60隻,隨機分為正常對照組和糖尿病組.後者通過腹腔註射鏈脲佐菌素(STZ)建立DR模型.採用流式細胞儀分彆計數建模後1週、1、3、6箇月各組大鼠循環外週血EPCs數量,同時于各相應時間點摘除大鼠眼毬行囌木精一伊紅(HE)染色、視網膜血管消化鋪片過碘痠-Schiff(PAS)染色,通過光學顯微鏡以及透射電子顯微鏡進行視網膜組織病理學檢查.對比分析EPCs數量以及EPCs數量與DR形態學變化的相互關繫.結果 大鼠註射STZ 1週、1、3、6箇月後循環外週血EPCs數量分彆為(25±7)、(28±8)、(39±7)、(43±7)箇/20萬箇單箇覈細胞,對照組為(45±4)箇120萬箇單箇覈細胞.與正常對照組大鼠相比,糖尿病組EPCs數量均降低(F=8.933,P＜0.01).隨著觀察時間的延長,糖尿病組EPCs數量逐漸迴升,而視網膜結構、光感受器細胞、血管也逐漸齣現病理性改變.光學顯微鏡下,與正常對照組比較,大鼠註射STZ後1週和1箇月組視網膜各層結構清晰完整,但厚度開始變薄,1箇月組視網膜細胞排列紊亂,細胞覈腫脹、體積增大,光感受器細胞減少,隨病程時間增加這些改變逐漸加重,3、6箇月組視網膜厚度更薄,細胞排列紊亂更加明顯,部分擴張的血管有嚮外突齣、破潰的趨勢.透射電子顯微鏡下,毛細血管內皮細胞水腫,線粒體腫脹;毛細血管基底膜增厚,管腔狹窄,6箇月組甚至齣現管腔閉塞和視網膜微動脈瘤;光感受器細胞膜盤間隙擴大,排列紊亂,線粒體水腫,細胞早期空泡變性.結論 糖尿病大鼠外週血EPCs數量較正常大鼠降低.隨著DR的進展,EPCs數量逐漸迴升.
목적 관찰당뇨병시망막병변(DR)대순배외주혈내피조세포(EPCs)적영향.방법 웅성성년Wistar대서60지,수궤분위정상대조조화당뇨병조.후자통과복강주사련뇨좌균소(STZ)건립DR모형.채용류식세포의분별계수건모후1주、1、3、6개월각조대서순배외주혈EPCs수량,동시우각상응시간점적제대서안구행소목정일이홍(HE)염색、시망막혈관소화포편과전산-Schiff(PAS)염색,통과광학현미경이급투사전자현미경진행시망막조직병이학검사.대비분석EPCs수량이급EPCs수량여DR형태학변화적상호관계.결과 대서주사STZ 1주、1、3、6개월후순배외주혈EPCs수량분별위(25±7)、(28±8)、(39±7)、(43±7)개/20만개단개핵세포,대조조위(45±4)개120만개단개핵세포.여정상대조조대서상비,당뇨병조EPCs수량균강저(F=8.933,P＜0.01).수착관찰시간적연장,당뇨병조EPCs수량축점회승,이시망막결구、광감수기세포、혈관야축점출현병이성개변.광학현미경하,여정상대조조비교,대서주사STZ후1주화1개월조시망막각층결구청석완정,단후도개시변박,1개월조시망막세포배렬문란,세포핵종창、체적증대,광감수기세포감소,수병정시간증가저사개변축점가중,3、6개월조시망막후도경박,세포배렬문란경가명현,부분확장적혈관유향외돌출、파궤적추세.투사전자현미경하,모세혈관내피세포수종,선립체종창;모세혈관기저막증후,관강협착,6개월조심지출현관강폐새화시망막미동맥류;광감수기세포막반간극확대,배렬문란,선립체수종,세포조기공포변성.결론 당뇨병대서외주혈EPCs수량교정상대서강저.수착DR적진전,EPCs수량축점회승.
Objective To observe the effect of diabetic retinopathy on endothelial progenitor cells (EPCs)from peripheral blood.Methods Sixty male Wistar rats were divided into control group and diabetes group.The rats in diabetes group were induced with streptozotocin(STZ)injection for diabetic retinopathy model.Flow cytometry was used to identify and count the number of EPCs from peripheral blood at 1 week.1,3 and 6 months after injection.All eyeballs were examined by hematoxylin and eosin (HE)staining,periodic acid-Schiffs(PAS)staining of trypsin-digested retinal vessels flat preparation and transmission electron microscope.EPCs count,and the relationship between DR morphological changes and EPCs count were compared and analyzed.Results The quantity of EPCs from peripheral blood at 1 week,1,3 and 6 months after STZ injection were 25±7,28±8,39±7,43±7 cells per 200 000 monocytes respectively,which decreased compared with the control group 45±4 cells per 200 000 monocytes(F=8.933,P＜0.0 1).The quantity of EPCs was gradually increased at 1 week,1,3 and 6 months after STZ injection,accompanied with responsive pathological changes of retinal structure and vessels.The thickness of retina at 1 week and 1 month after injection were reduced slightly.The number of retinal ganglion cells reduced,with the time passing by.Endothelial cells were edema,mitochondrial was swollen,capillary basement membrane was thicken,lumen was significant stenosis,lumen occlusion and retinal artery aneurysm were observed at 6 months after STZ injection.Conclusion The number of EPCs increases gradually throughout the development of DR.
