CAJ | 학술논문

哺乳动物卵母细胞是发育生物学和细胞生物学的重要研究对象.激光共聚焦显微术是检测卵母细胞中纺锤体相关蛋白的有用技术,但是针对哺乳动物卵母细胞的特点需要做许多调整以得到理想结果.其中,卵母细胞的准备、固定、透膜、脂类抽提、抗体孵育步骤十分关键.另外,不同物种的卵母细胞也有差异,在进行激光共聚焦研究时需要做出相应的调整.
포유동물란모세포시발육생물학화세포생물학적중요연구대상.격광공취초현미술시검측란모세포중방추체상관단백적유용기술,단시침대포유동물란모세포적특점수요주허다조정이득도이상결과.기중,란모세포적준비、고정、투막、지류추제、항체부육보취십분관건.령외,불동물충적란모세포야유차이,재진행격광공취초연구시수요주출상응적조정.
The mammalian oocytes are important materials for study of meiotic cell cycle in developmental biology and cell biology. Confocal microscopy is a powerful technique in detecting the spindle-associated proteins in oocytes. However, in order to obtain the ideal results, some technological modifications should be made according to the characteristics of mammalian oocytes. Among them, the oocyte preparation, fixation, membrane permeabilization, fat extraction, and antibody incubation are the key steps. Furthermore, the differences among oocytes from various mammalian species should be considered in the actual manipulation of confocal microscopy.